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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model

  • Kim, Dae-Won (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Hwang, Hyun-Sook (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Kim, Duk-Soo (Department of Anatomy, College of Medicine, Soonchunhyang University) ;
  • Sheen, Seung-Hoon (Department of Neurosurgery, Hallym University Medical Center) ;
  • Heo, Dong-Hwa (Department of Neurosurgery, Hallym University Medical Center) ;
  • Hwang, Gyo-Jun (Department of Neurosurgery, Hallym University Medical Center) ;
  • Kang, Suk-Hyung (Department of Neurosurgery, Hallym University Medical Center) ;
  • Kweon, Hae-Yong (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Jo, You-Young (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Seok-Woo (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwang-Gill (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Park, Kye-Won (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Han, Kyu-Hyung (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Eum, Won-Sik (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Cho, Yong-Jun (Department of Neurosurgery, Hallym University Medical Center) ;
  • Choi, Hyun-Chul (Department of Neurosurgery, Hallym University Medical Center) ;
  • Choi, Soo-Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
  • Received : 2011.08.22
  • Accepted : 2011.08.30
  • Published : 2011.12.31
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Abstract

We investigated whether silk fibroin peptide derived from the silkworm, Bombyx mori, could inhibit inflammation and enhance the anti-inflammatory activity of Tat-superoxide dismutase (Tat-SOD), which was previously reported to effectively penetrate various cells and tissues and exert anti-oxidative activity in a mouse model of inflammation. Inflammation was induced by topical treatment of mouse ears with 12-O-tetradecanoylphorbol-13-acetate (TPA). Histological, Western blot, and reverse transcription-polymerase chain reaction data demonstrated that silk fibroin peptide or Tat-SOD alone could suppress elevated levels of cyclooxygenase-2, interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha induced by TPA. Moreover, silk fibroin peptide significantly enhanced the anti-inflammatory activity of Tat-SOD, although it had no influence on in vitro and in vivo transduction of Tat-SOD. Silk fibroin peptide exhibited anti-inflammatory activity in a mice model of inflammation. Therefore, silk fibroin peptide alone or in combination with Tat-SOD might be used as a therapeutic agent for various inflammatory diseases.

Keywords

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