Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Characterization of Two Ricin Isoforms by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis and Capillary Isoelectric Focusing

  • Na, Dong-Hee (College of Pharmacy, Kyungsung University) ;
  • Park, Eun-Ji (College of Pharmacy, Kyungsung University) ;
  • Kim, Myung-Sun (College of Pharmacy, Kyungsung University) ;
  • Cho, Cheong-Kwan (Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University) ;
  • Woo, Byung-Ho (Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University) ;
  • Lee, Hye-Suk (College of Pharmacy, The Catholic University of Korea) ;
  • Lee, Kang-Choon (Drug Targeting Laboratory, College of Pharmacy, SungKyunKwan University)
  • Received : 2011.05.12
  • Accepted : 2011.10.09
  • Published : 2011.12.20
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Abstract

Sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) and capillary isoelectric focusing (CIEF) were employed to characterize and compare ricin E purified from the small grain seeds of Ricinus communis with ricin D isoform. During the purification of ricin E using ion-exchange and size-exclusion chromatography, SDS-CGE was found to be useful for monitoring the efficiencies of purification steps. SDS-CGE showed that the molecular size of ricin E was not significantly different from that of ricin D, which was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. CIEF was useful for discriminating ricin E from ricin D based on their isoelectric points (pI). The pI values of ricin E and D were 8.6-8.8 and 7.0-7.4, respectively. This study demonstrates the usefulness of SDS-CGE and CIEF for the characterization of ricin toxins.

Keywords

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