Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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The Role of Transglutaminase in Double-stranded DNA-Triggered Antiviral Innate Immune Response

  • Yoo, Jae-Wook (Global Research Laboratory for RNAi Medicine, Department of Chemistry, Sungkyunkwan University) ;
  • Hong, Sun-Woo (Global Research Laboratory for RNAi Medicine, Department of Chemistry, Sungkyunkwan University) ;
  • Bose, Shambhunath (Institute of Medical Research, College of Medicine, Dongguk University) ;
  • Kim, Ho-Jun (Institute of Medical Research, College of Medicine, Dongguk University) ;
  • Kim, Soo-Youl (Cancer Cell and Molecular Biology Branch, Division of Cancer Biology, Research Institute) ;
  • Kim, So-Youn (Department of Biomedical Engineering, Dongguk University) ;
  • Lee, Dong-Ki (Global Research Laboratory for RNAi Medicine, Department of Chemistry, Sungkyunkwan University)
  • Received : 2011.08.26
  • Accepted : 2011.09.02
  • Published : 2011.11.20
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Abstract

Cellular uptake of double-stranded DNA (dsDNA) triggers strong innate immune responses via activation of NF-${\kappa}B$ transcription factor. However, the detailed mechanism of dsDNA-mediated innate immune response remains yet to be elucidated. Here, we show that the expression of tazarotene-induced gene 3 (TIG3) is dramatically induced by dsDNA stimulation, and the siRNA-mediated down-regulation of TIG3 mRNA results in significant suppression of dsDNA-triggered cytokine expression. Because TIG3 has been previously shown to physically interact with transglutaminase (TG) 1 to activate TG activity, and TG2 has been shown to induce NF-${\kappa}B$ activity by inducing $I{\kappa}B{\alpha}$ polymerization, we tested whether TG also plays a role in dsDNA-mediated innate immune response. Pre-treatment of TG inhibitors dramatically reduces dsDNA-triggered cytokine induction. We also show that, in HeLa cells, TG2 is the major TG, and TIG3 physically interacts with TG2. Combined together, our results suggest a novel mechanism of dsDNA-triggered innate immune response which is critically dependent on TIG3 and TG2.

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References

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