Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Detection of IgG Using Thiolated Protein G Modified SPR Sensor Chip

Thiolated protein G로 개질된 SPR 센서 칩을 이용한 IgG 검출

  • Sin, Eun-Jung (Department of Biomedical Engineering, Catholic University of Daegu) ;
  • Lee, Yeon-Kyung (Department of Biomedical Engineering, Catholic University of Daegu) ;
  • Sohn, Young-Soo (Department of Biomedical Engineering, Catholic University of Daegu)
  • 신은정 (대구가톨릭대학교 의공학과) ;
  • 이연경 (대구가톨릭대학교 의공학과) ;
  • 손영수 (대구가톨릭대학교 의공학과)
  • Received : 2011.10.07
  • Accepted : 2011.11.08
  • Published : 2011.11.30
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Abstract

A portable surface plasmon resonance(SPR) based immunosensor using thiolated protein G and protein G was developed for the detection of immunoglobulin G(IgG). The protein G has specific affinity with Fc fragment of IgG and was thiolated by 2-Iminothiolane for introduction of thiol groups. Anti-IgG, bovine serum albumin(BSA), and IgG have been sequently injected after surface modification of gold sensor chip with protein G and thiolated protein G. The output signal was increased with the injection of each protein and the actual signal was measured by subtracting signal of reference channel from signal of sample injected channel. The experimental results showed the higher detection capability of IgG using thiolated protein G compared with protein G. From these results, we can conclude that the current surface modification technique and the portable SPR sensor system can be applied to various immunosensors for diagnosis.

Keywords

References

  1. 성건용, 박찬우, 김경현, 양종헌, "유비쿼터스 건강관리를 위한 바이오센서 기술 동향", 전자 통신동향분석, 제24권, 제5호, pp. 38-51, 2009.
  2. 조재형, 권혁상, 윤건호,""Ubiquitous Health Care System"을 이용한 미래형 당뇨병 관리에 대한 전망", 당뇨병, 제30권, 제2호, pp. 87-95, 2006. https://doi.org/10.4093/jkda.2006.30.2.87
  3. 김의락, 백세환, "면역센서의 원리와 의학적 응용", 한국생물공학회지, 제17권, 제2호, pp. 121-136, 2002.
  4. K. A. Mark, "Quartz crystal microbalance: A useful tool for studying thin polymer films and complex biomolecular systems at the solution-surface Interface", Biomacromolecules, vol. 4, no. 5, pp. 1099-1120, 2003. https://doi.org/10.1021/bm020116i
  5. N. Trummer, N. Adanyi, M. Varadi, and I. Szendro, "Modification of the surface of integrated optical wave-guide sensors for immunosensor applications", Fresenius J. Anal. Chem., vol. 371, no. 1, pp. 21-24, 2001. https://doi.org/10.1007/s002160100929
  6. R. Kurrat, B. Walivaara, A. Marti, M. Textor, P. Tengvall, J.J. Ramsden, and N.D. Spencer, "Plasma protein adsorption on titanium: comparative in situ studies using optical waveguide lightmode spectroscopy and ellipsometry", Colloids Surf. B. Biointerfaces, vol. 11, no. 4, pp. 187-201, 1998. https://doi.org/10.1016/S0927-7765(98)00039-3
  7. K. Hegnerova, M. Bockova, H. Vaisocherova, Z. Kristofikova, J. Ricny, D. Ripova, and J. Homola, "Surface plasmon resonance biosensors for detection of Alzheimer disease biomarker", Sens. Actuators B Chem., vol. 139, no. 1, pp. 69-73, 2009. https://doi.org/10.1016/j.snb.2008.09.006
  8. C. Bian, J. Tong, J. Sun, H. Zhang, Q. Xue, and S. Xia, "A field effect transistor(FET)-based immunosensor for detection of HbA1c and Hb", Biomed. Microdevices, vol. 13, no. 2, pp. 345-352, 2011. https://doi.org/10.1007/s10544-010-9498-y
  9. M. Salmain, N. Fischer-Durand, and C.M. Pradier, "Infrared optical immunosensor: application to the measurement of the herbicide atrazine", Anal. Biochem., vol. 373, no. 1, pp. 61-70, 2008. https://doi.org/10.1016/j.ab.2007.10.031
  10. Sung-Sik Yun, Soo hyun Lee, Chong H. Ahn, and Jong-Hyun Lee, "A fiber optic surface Plasmon resonance(SPR) sensor using cyclic olefin copolymer(COC) polymer prism", J. Kor. Sensors Soc., vol. 17, no. 5, pp. 369-374, 2008. https://doi.org/10.5369/JSST.2008.17.5.369
  11. P. Englebienne, A. Van Hoonacker, and M. Verhas, "Surface plasmon resonance: principles, methods and applications in biomedical sciences", Spectroscopy, vol. 17, pp. 255-273, 2003. https://doi.org/10.1155/2003/372913
  12. K. S. Lee, J. M. Son, D. Y. Jeong, T. S. Lee, and W. M. Kim, "Resolution enhancement in surface plasmon resonance sensor based on waveguide coupled mode by combining a bimetallic approach", Sensors, vol. 10, no. 12, pp. 11390-11399, 2010. https://doi.org/10.3390/s101211390
  13. A. M. Bond, "Past, present and future contributions of microelectrodes to analytical studies employing voltammetric detection. A review", Analyst, vol. 119, issue. 11, p. 1R, 1994. https://doi.org/10.1039/an994190001r
  14. Y. M. Bae, B.-K. Oh, W. C. Lee, W. H. Lee, J.-W. Choi, and Young Min Bae, "Study on orientation of immunoglobulin G on protein G layer", Biosens. Bioelectron., vol. 21, no. 1, pp. 103-110, 2005. https://doi.org/10.1016/j.bios.2004.09.003
  15. M. Suzuki, F. Ozawa, W. Sugimoto, and S. Aso, "Miniature surface plasmon resonance immunosensors - rapid and repetitive procedure", Anal. Chim. Acta., vol. 404, no. 2, pp. 187-194, 2000. https://doi.org/10.1016/S0003-2670(99)00718-7
  16. 최효진, 황상연, 장대호, 조형민, 강정혜, 성 기훈, 주재범, 이은규, "자성 미세입자에의 항체 고정화 방법이 면역결합반응에 미치는 영향", 화학공학, 제44권, 제1호, pp. 65-72, 2006.