Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Thirteen-Week Oral Dose Toxicity Study of G. bimaculatus in Sprague-Dawley Rats

  • Ahn, Mi-Young (Department of Agricultural Biology, National Academy of Agricultural, RDA) ;
  • Han, Jea-Woong (Department of Agricultural Biology, National Academy of Agricultural, RDA) ;
  • Kim, Soon-Ja (Department of Agricultural Biology, National Academy of Agricultural, RDA) ;
  • Hwang, Jae-Sam (Department of Agricultural Biology, National Academy of Agricultural, RDA) ;
  • Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural, RDA)
  • Received : 2011.06.09
  • Accepted : 2011.11.15
  • Published : 2011.12.01
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Abstract

Gryllus bimaculatus (Gb) was orally administered at doses of 0, 0.04, 0.2, 1 and 5 g/kg bw/day for 13 consecutive weeks. There were no observed clinical signs or deaths related to treatment in all the groups tested. Therefore, the approximate lethal oral dose of G. bimaculatus was considered to be higher than 5 g/kg in rats. Throughout the administration period, no significant changes in diet consumption, ophthalmologic findings, organ weight, clinical pathology (hematology, clinical chemistry, coagulation, and urinalysis) or gross pathology were detected. Minor changes were found in hematological parameters for the 5 g/kg Gb-treated group (triglyceride reduction of 35.8%), but all changes were within normal physiological ranges. Microscopic examination did not identify any treatment-related histopathologic changes in the organs of Gb-treated rats in the high dose group. From these results, one can conclude that the no-observed adverse effect level (NOAEL) of G. bimaculatus is higher than 5 g/kg bw/day in rats.

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References

  1. Ahn, M.Y., Ryu, K.S., Park, B.Y., Kim, D.W., Kim, I.S. and Kim, S.H. (2000). Effects of cricket supplements on the chicken meats and its eggs. Korean J. Poult. Sci., 27, 169-282.
  2. Ahn, M.Y., Lee,Y.W., Ryu, K.S., Kim, I. K., Kim, J.W., Lee, Y.K., Kim, E.S., Kim, Y.S. and Lee, H.S. (2002). Protective effects of water/methanol extracts of cricket on the acute hepatic damages in the ICR-mice induced by administration of $CCl_4$. Korean J. Food Sc. Technol., 34, 684-687.
  3. Ahn, M.Y., Lee, Y.W., Ryu, K.S., Lee, H.S., Kim, I.K., Kim, J.W. and Lim, S.S. (2004). Effect of water and methanol extracts of cricket (Gryllus bimaculatus) on alcohol metabolism. Kor. J. Pharmacogn., 35, 175-178.
  4. Ahn, M.Y., Bae, H.J., Kim, I.S., You, E.J., Kwack, S.J., Kim, H.S., Kim, D.H., Ryu, K.S., Lee, H.S., Kim, J.W., Kim, I. and Lee, B.M. (2005). Genotoxic evaluation of the biocomponents of the cricket, Gryllus bimaculatus, using three mutagenicity tests. J. Toxicol. Environ. Health A, 68, 2111-2118. https://doi.org/10.1080/15287390500182537
  5. Cui, Z., Ahn, M.Y., Lee, Y.B. and Ryu, K.S. (2002). Materia medica from insects. Shinilbooks. Seoul, pp. 137-145.
  6. Hwang, S.Y., Sin, J.S., Kwon, W., Chai, H.Y., Cho, J.H., Lee, N.J., Park, J.B., Kim, I., Ryu, K.S., Yun, C.Y., Kang, J.K. and Kim, Y.B. (2004). Repeated-dose toxicity study for the extract of cricket, Gryllus bimaculatus, in rat. The Korean Journal of Laboratory Animal Science, 20, 113-209.
  7. Kim, I.S., Ahn, M.Y., Ryu, K.S. and Lee, B.M. (2002). Acute oral toxicity of G. bimaculatus in rats. J. Toxicol. Pub. Health, 18, 397-400.
  8. Kwon, W., Chai, H.Y., Cho, Y.M., Chio, E.K., Sin, J.S., Kim, T.M., Kim, I., Hwang, S.Y. and Yun, C.Y. (2004). Protective effects of extract of cricket, Gryllus bimaculatus, against hepatotoxicity induced by 2,3,7,8-tetrachlorodibenzo-r-dioxin (TCDD) in rats. Korean J. Lab. Ani. Sci., 53, 1341-1357.
  9. Song, S.W., Jung, W. and Hong, D.H. (2006). Thirteen-week repeated-dose toxicity studies of STB-HO-BM in rats. J. Toxicol. Pub. Health, 22, 135-144.

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