The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Mutagenesis of of Hypsizygus marmoreus by Gamma Ray Irradiation

감마방사선 조사에 의한 느티만가닥버섯의 변이

  • Kim, Jong-Kun (Graduate School of Bio.&Information Technology, Hankyong National University) ;
  • Moon, Deok-Hun (Graduate School of Bio.&Information Technology, Hankyong National University) ;
  • Seo, Geon-Sik (Korea National college of Agricultural and Fisheries) ;
  • Kang, Hee-Wan (Graduate School of Bio.&Information Technology, Hankyong National University)
  • 김종군 (한경대학교 바이오.정보기술 전문대학원) ;
  • 문덕훈 (한경대학교 바이오.정보기술 전문대학원) ;
  • 서건식 (한국농수산대학 특용작물학과) ;
  • 강희완 (한경대학교 바이오.정보기술 전문대학원)
  • Received : 2011.11.16
  • Accepted : 2011.11.21
  • Published : 2011.12.01
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Abstract

This study was performed to develop new Hypsizygus marmoreus cultivars that have enhanced functional materials and improved physiological characteristics with mutagenesis by gamma ray irradiation. Protoplasts of H. marmoreus brown strain HYM-056 were irradiated by gamma ray for mutagenesis, and then 2,000 clones of mutants were randomly selected and the fruiting bodies were induced by bottle culture. Among them, 157 isolates with fast-growing, heavy and many fruiting body-producing were selected. The isolates were cultured in plastic bottle containing rice bran, barley hulls and fir sawdust to form the fruiting bodies. About 100 days after inoculation, characteristic of fruiting bodies were investigated. The isolates were divided into 6 groups based on color, shape and size of pileus, and length, diameter, number and weight of stipe. In addition, the genetic variation of the isolates was analyzed by URP-PCR fingerprinting.

본 연구는 감마방사선 조사에 의해 기능성 물질이 증가하고, 생리적 특성이 증진된 느티만가닥 버섯의 새로운 품종을 개발하기위하여 수행되었다. 돌연변이 유기를 위하여 느티만가닥 버섯의 갈색 계통 균주 HYM-056의 원형질에 감마방사선을 조사하여 2,000개의 돌연변이체를 무작위로 선발하고 병 재배하여 자실체를 형성시켰다. 이 중 생장속도가 빠르고, 중량이 무거우며, 자실체를 다량으로 생산하는 500개 균을 선발하였다. 선발된 균주의 자실체 형성을 위하여 미강, 보리껍질, 미송이 함유된 플라스틱 병에 재배하였다. 접종 100일 후, 자실체의 특성을 조사하였다. 그 결과 갓의 색깔, 형태, 크기와 대의 길이, 직경, 숫자, 무게 등에 따라 6개의 그룹으로 나뉘었다. 또한 URP-PCR 핵산 지문 분석으로 유전적 변이를 조사하였다.

Keywords

References

  1. 김종군, 김진희, 공원식, 강희완. 2010. 버섯류의 원형질체 나출을 위한 고효율 효소 선발. 한국균학회지 38(1):21-24. https://doi.org/10.4489/KJM.2010.38.1.021
  2. 임윤정, 이창윤, 박정은, 김상우, 이현숙, 노현수. 2010. 느티만가닥버섯의 분자유전학적 분류 및 품종특이적 DNA 마커 탐색. 한국균학회지 385(1):34-39. https://doi.org/10.4489/KJM.2010.38.1.034
  3. 정해영, 김계령. 2010. 이온빔을 이용한 미생물 균주 개량. Kor. J. Microbiol. Biotechnol. 38(3):235-243.
  4. Akavia, E., Beharav, A., Wasser, S. P. and Nevo, E. 2009. Disposal of agroindustrial by-products by organic cultivation of the culinary and medicinal mushroom Hypsizygus marmoreus. Waste Manage. 29(5):1622-1627. https://doi.org/10.1016/j.wasman.2008.10.024
  5. Djajanegara, I. and Harsoyo. 2008. White oyster mushroom (Pleurotus florida) mutant with altered antioxidant contents. Biotropia 15(1):65-73.
  6. Lee, Y.-K., Chang, H.-H., Kim, J.-S., Kim, J. K., and Lee, K.-S. 2000. Lignocellulolytic mutants of Pleurotus ostreatus induced by gamma-ray radiation and their genetic similarities. Radiation Physics and Chemistry 57(2):145-150. https://doi.org/10.1016/S0969-806X(99)00310-2
  7. Matsuzawa, T., Saitoh, H., Sano, M., Tomita, I., Ohkawa, M. and Ikekawa, T. 1998. Studies on antioxidant effects of Hypsizigus marmoreus. II. Effects of Hypsizigus marmoreus for antioxidant activities of tumorbearing mice. Yakugaku Zasshi, 118(10):476-481. https://doi.org/10.1248/yakushi1947.118.10_476
  8. Mori, K., Kobayashi, C., Tomita, T., Inatomi, S. and Ikeda, M. 2008. Antiatherosclerotic effect of the edible mushrooms Pleurotus eryngii (Eringi), Grifola frondosa (Maitake), and Hypsizygus marmoreus (Bunashimeji) in apolipoprotein E-deficient mice. Nutr. Res. 28(5):335-342. https://doi.org/10.1016/j.nutres.2008.03.010
  9. Park, J. Y., Oh, W.-J., Kwak, D.-M., Kim, M.-G., Seo, G.-S., Hong, S.-B. and Rhee, M. H. 2011. The anti-platelet activity of Hypsizygus marmoreus extract is involved in the suppression of intracellular calcium mobilization and integrin $<\alpha}IIb{\beta}3$ activation. Journal of Medicinal Plants Research 5(11): 2369-2377.

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  1. Mutagenesis of Pleurotus eryngii by Gamma Ray Irradiation vol.40, pp.2, 2012, https://doi.org/10.4489/KJM.2012.40.2.93
  2. Genetic variation of local varieties and mutants groups induced by gamma ray in Hypsizigus marmoreus vol.12, pp.3, 2014, https://doi.org/10.14480/JM.2014.12.3.181
  3. Genetic Variation in Mutants Induced by Gamma Ray in Hypsizigus marmoreus vol.24, pp.11, 2014, https://doi.org/10.5352/JLS.2014.24.11.1174