핑크팝 보리수 열매와 잎 용매 분획물의 항산화 활성

Antioxidative Activities of Various Solvent Fractions from Fruit and Leaf of Pinkpop Borisu

  • 강선경 (경상대학교 식품공학과 (농업생명과학연구원)) ;
  • 정창호 (경상대학교 식품공학과 (농업생명과학연구원)) ;
  • 허호진 (경상대학교 식품공학과 (농업생명과학연구원)) ;
  • 심기환 (경상대학교 식품공학과 (농업생명과학연구원))
  • Kang, Sun-Kyung (Dept. of Food Sci. & Techn. (Insti. of Agric. & Life Sci.), Gyeongsang National Univ.) ;
  • Jeong, Chang-Ho (Dept. of Food Sci. & Techn. (Insti. of Agric. & Life Sci.), Gyeongsang National Univ.) ;
  • Heo, Ho-Jin (Dept. of Food Sci. & Techn. (Insti. of Agric. & Life Sci.), Gyeongsang National Univ.) ;
  • Shim, Ki-Hwan (Dept. of Food Sci. & Techn. (Insti. of Agric. & Life Sci.), Gyeongsang National Univ.)
  • 투고 : 2010.04.07
  • 심사 : 2010.06.15
  • 발행 : 2010.06.30

초록

핑크팝 보리수 열매와 잎을 기능성 식품의 재료로 활용하기 위하여 항산화 및 총 페놀성 화합물 함량을 측정한 결과는 다음과 같다. DPPH 및 ABTS radical 소거활성을 측정한 결과 분획물의 농도가 증가함에 따라 DPPH 및 ABTS radical 소거활성이 증가하는 경향을 보였으며, 특히 잎의 부탄올 분획물에서 가장 높은 DPPH radical 소거활성을 보였다. 또한 ABTS 라디칼 소거활성도 열매와 잎의 여러 분획물 중 잎의 부탄올 분획물에서 가장 높은 소거활성을 보였다. 환원력과 FRAP 활성도 분획물의 농도가 증가함에 따라 환원력과 FRAP 활성이 증가하는 경향을 보였으며, 잎의 부탄올 분획물에서 가장 높은 환원력과 FRAP 활성을 보였다. Linoleic acid를 이용한 자동산화 억제활성을 실험한 결과 다른 분획물에 비하여 부탄올 분획물에서 가장 높은 과산화 억제활성을 나타내었으며, 총 페놀성 화합물은 잎과 열매의 부탄올 분획물에서 각각 106.34 및 252.46 mg/g으로 가장 높은 함량을 보였다. 핑크팝 보리수 잎 부탄올 분획물의 항산화 활성은 페놀성 화합물에 의한 것이며, 이는 자유 라디칼에 의해 유발된 산화적 스트레스를 효과적으로 방어할 수 있을 것이다.

To get basic data for the utilization of Pinkpop Borisu fruit and leaf as a raw material in functional food, antioxidative activities were investigated. The radical scavenging activity of various solvent fraction from fruit and leaf against the DPPH and ABTS radicals increased with the increasing amount of various solvent fractions and butanol fraction of leaf showed the highest DPPH radical scavenging activities. Among the various solvent fractions from fruit and leaf of Pinkpop Borisu, butanol fraction of leaf showed the strongest scavenging effect on ABTS radicals. Reducing power and ferric reducing/antioxidant power (FRAP) of the various solvent fractions from fruit and leaf increased with the increasing concentration of fractions and butanol fraction of leaf showed the highest reducing power. The butanol fraction of fruit and leaf showed strong antioxidative activity on linoleic acid system. Total phenolics contents of butanol fraction from fruit and leaf were 106.34 and 252.46 mg/g, respectively. The antioxidative activities of the butanol fraction from fruit and leaf are attributed to phenolic compounds. The present results suggest that butanol fraction of Pinkpop Borisu leaf would have the protective potential from oxidative stress induced by free radicals.

키워드

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