Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

The Effect of Moisture Control on Fermentation Characteristics of Barley and Rye Silages

수분 조절이 보리와 호밀 silage의 발효특성에 미치는 영향

  • Lee, Jong-Chan (Daesung Microbiological Labs. Co., Ltd.) ;
  • Kim, Sam-Churl (Dept. of Animal Sci(Insti. of Agric. & Life Sci.)., Gyeongsang National Univ.)
  • 이종찬 (대성미생물 연구소) ;
  • 김삼철 (경상대학교 축산학과(농업생명과학연구원))
  • Received : 2010.01.28
  • Accepted : 2010.02.22
  • Published : 2010.02.28
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Abstract

This study examined the effect of moisture level at ensiling on fermentation characteristics of barley and rye silage. The moisture levels, 60 (low; LM), 70(medium; MM) and 80% (high; HM), were controled by adding water or pre-wilting. Barley silage had higher pH and latate:acetate ratio in LM than the other treatments, but those of rye in MM were higher. The concentrations of lactate, total FA and acetate in HM were higher than the other treatments, but propionate concentration in LM was higher than HM. Total N concentrations of barley and rye were highest in MM and LM, respectively. The $NH_3-N$ concentration and total N:$NH_3-N$ ratio of barley were higher in HM than those in LM and MM. With increasing moisture content, buffering capacity of barley and rye silages increased, whereas decreased by increase of pH. There was a negative correlation between moisture content vs. pH of barley and rye silages. However, moisture content vs. the concentrations of total VFA and $NH_3-N$ and the ratio of total N:$NH_3-N$ had a positive correlation. Tn conclusion, the ideal moisture content of barley and rye for silage was 70-80%, but silage quality could be rapidly decreased by pre-wilting to 60% moisture content.

본 연구는 수분함량에 따른 보리와 호밀 silage의 발효특성을 평가하기 위하여 수행하였다. 보리와 호밀의 수분함량은 수분첨가 또는 예건을 통해 50%, 65% 및 80%로 조절하였다. 보리 silage의 pH와 Lactate:Acetate 비율은 저수분 처리구 (4.54와 6.23), 호밀 silage에서는 중수분 처리구 (4.33과 9.24)가 가장 높았다. 보리 silage의 propionate 함량은 저수분 처리구가 고수분 처리구에 비해 높았으나, 보리와 호밀 silage의 lactate, 총휘발성지방산 및 acetate 함량은 고수분 처리구가 저수분 처리구에 비해 높았다. 보리 silage의 total-N 함량은 중수분 처리구가 저수분 처리구에 비해 높게 나타났으며, 호밀 silage는 저수분 처리구가 타 처리구에 비해 높았다. 보리 silage의 $NH_3-N$ 함량과 total-N:$NH_3-N$ 비율은 고수분 처리구가 높게 나타났다. 보리와 호밀 silage의 buffering capacity는 수분함량이 높을수록 증가하였으나, pH가 높을수록 감소하였다. 보리와 호밀 silage의 수분 함량은 pH와는 부(-)의 상관관계를 나타내었으나, 휘발성지방산과 $NH_3-N$ 함량 및 total-N중 $NH_3-N$ 비율과는 정 (+)의 상관관계를 나타내었다. 이상의 결과에서 보리와 호밀 silage 제조를 위한 적정 수분함량은 70~80%로 확인되었으며, 사료작물의 수분을 60%까지로 예건하는 것은 silage 품질 저하를 초래할 수 있는 것으로 사료된다.

Keywords

References

  1. A.O.A.C. 1990. Official methods of analysis. Association of Official Agricultural Chemist, Washington, D. C.
  2. Barnett, A. J. G. 1951. The colorimetric determination of lactic acid in silage. Biochem. J. 49: 527-529. https://doi.org/10.1042/bj0490527
  3. Bolsen, K. K. and J. L. Heidker. 1985. Silage Additives USA. Chalcombe Publ., Church Lane, Kingston, Canterbury, Kent, UK.
  4. Chaney, A. L. and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8: 130-132.
  5. Erwin, E. S., J. Marco, and E. M. Emery. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44: 1768-1771. https://doi.org/10.3168/jds.S0022-0302(61)89956-6
  6. Fenner, H. and J. M. Elliot. 1963. Quantitative method for determining the steam volatile fatty acids in the rumen fluid by gas chromatography. J. Anim. Sci. 22: 624-627. https://doi.org/10.2527/jas1963.223624x
  7. Flieg, O. 1938. A key for the evaluation of silage samples. Futterbau und Giirfutterbereitung 1: 112-128.
  8. Gordon, C. H. 1967. Storage losses in silage as affected by moisture content and structure. J. Dairy Sci. 50: 398-402.
  9. Jang, J. H., S. Y. Han, and D. J. Kim. 1995. Effects of wheat bran addition on the quality of rye silage. J. Kor. Grassl. Forage Sci. 15: 285-290.
  10. Kang, T. W., A. T. Adesogan, S. C. Kim, and S. S. Lee. 2009. Effects of an esterase-producing inoculant on fermentation, aerobic stability, and neutral detergent fiber digestibility of corn silage. J. Dairy Sci. 92: 732-738. https://doi.org/10.3168/jds.2007-0780
  11. Kim, J. G., M. S. Han, G. Y. Kim, J. D. Han, H. S. Jin, and C. N. Shin. 1996. Study on baled silage making of selected forage crop and pasture grasses III. Evaluation of chemical components and energy contents of baled silage with selected forages. J. Kor. Grassl. Forage Sci. 16: 87-92.
  12. Kim, J. H. 1993. Studies on nutrition and feed value of the whole crop corn ensiled with cage layer manure for ruminants. Gyeongsang National University in partial fulfillment of the requirements for the degree of Doctor of Science.
  13. Kim, S. C. and A. T. Adesogan. 2006. Influence of ensiling temperature, simulated rainfall, and delayed sealing on fermentation characteristics and aerobic stability of corn silage. J. Dairy Sci. 89: 3122-3132. https://doi.org/10.3168/jds.S0022-0302(06)72586-3
  14. Ko, Y. D., C. H. Kwack, and Y. S. Moon. 1987. Study on the quality of whoie crop silage and yield by stage of maturity of rye plant II. Quality of whole crop rye silage by stage of maturity. J. Kor. Grassl. Forage Sci. 7: 153-156.
  15. Lee, K. Y. and D. A. Kim. 1997. Effects of wilting and additives on the fermentation chrateristics, quality and aerobic stability of rye silage. J. Kor. Grassl. Forage Sci. 17: 187-198.
  16. McDonald, P., A. R. Henderson, and S. J. E. Heron. 1991. The biochemistry of silage (2nd ed.). Chalcombe Publ., Church Lane, Kingston, Canterbury, Kent, UK.
  17. Patterson, D. C. and J. P. Frost. 1998. Reseach seminar on ruminant production. In : A research update on wilted silage. Agricultural research institute of northern ireland. pp. 21-32.
  18. SAS Institute. 2002. User's Guide: Statistics. SAS Inst., Inc., Cary, NC.
  19. Shin, C. N. 1975. Effects of wilting on quality of grass silage. Kor. J. Anim. Sci. 17: 539-548.
  20. Shin, C. N. and D. H. Bae. 1986. Effects of formic acid on the quality of rye silages at different dry matter levels. J. Kor. Grassl. Forage Sci. 6: 6-13.
  21. Shin, C. N., and I. S. Yoon. 1983. Effects of wilting on silage quality. J. Kor. Grassl. Forage Sci. 3: 92-99.
  22. Yan, T., D. C. Patterson, F. J, Gordon, and M. G. Porter. 1996. The effects of wilting of grass prior to ensiling on the response to bacterial inoculation. 1. Silage fermentation and nutrient utilization over three harvests. Animal Science. 62: 405-417. https://doi.org/10.1017/S1357729800014958
  23. 고영두, 강우성, 강한석, 김대진, 김두환, 김재황, 남기홍, 류영우, 송영민, 신정남, 유성오. 1999. 반추가축을 위한 사일리지 제조 전략. 선진문화사.