FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
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Analysis of Genetic Relationship among Cymbidium germplasms Using RAPD and URP

RAPD와 URP를 이용한 심비디움 유전자원 유연관계 분석

  • Park, Pue Hee (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Mi Seon (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Young Ran (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Pil Man (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Dong Soo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yae, Byeong Woo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 박부희 (국립원예특작과학원 화훼과) ;
  • 김미선 (국립원예특작과학원 화훼과) ;
  • 이영란 (국립원예특작과학원 화훼과) ;
  • 박필만 (국립원예특작과학원 화훼과) ;
  • 이동수 (국립원예특작과학원 화훼과) ;
  • 예병우 (국립원예특작과학원 화훼과)
  • Received : 2010.08.02
  • Accepted : 2010.09.29
  • Published : 2010.09.30
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Abstract

The genetic relationship among 48 Cymbidium cultivars was analyzed using randomly amplified polymorphic DNA (RAPD) with eighty 10 mers random primers (Operon Technologies) and twelve 20 mers random primers. Forty eight Cymbidium cultivars included 34 oriental Cymbidium, 7 hybrids, and 7 western Cymbidium. 407 (9.9 per primer) and 56 polymorphic bands (9.5 per primer) were generated by polymerase chain reaction with selected thirty 10 mers primers, and nine 20 mers primers, respectively. The polymorphic fragments ranged from 0.4 to 1.5 kb in size. The dendrogram was constructed by using the UPGMA clustering algorithm based on genetic similarity. Forty eight Cymbidium cultivars were classified into four major groups at similarity coefficient value of 0.638.

심비디움속 유전자원 48품종에 대하여 RAPD와 URP를 이용하여 유전적 유연관계를 분석하였다. RAPD분석에는 10mer에 해당하는 random primer (Operon사) 80개를, URP는 20 mer에 해당하는 12종의 상용 primer를 이용하였다. 48 품종의 심비디움에는 34종의 동양 심비디움, 7종의 동서양란 교잡종, 7종의 서양 심비디움이 포함되어 있다. 선별된 41개의 random primer와 6개의 URP primer로부터 각각 407, 56개의 다형성 밴드를 획득하여 총 463개의 마커를 이용하였다. 이들 마커의 크기 범위는 0.4 kb 에서 1.5 kb 에 해당하였다. 유전적 유사도를 바탕으로 UPGMA clustering 프로그램을 이용하여 dendrogram을 작성하였는데 유전자원 48품종은 유사도 0.638 수준에서 총 4그룹으로 구분되었다.

Keywords

References

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