공업화학 (Applied Chemistry for Engineering)

  • 제21권6호
  • /
  • Pages.659-663
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  • 2010
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  • 1225-0112(pISSN)
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  • 2288-4505(eISSN)
한국공업화학회 (The Korean Society of Industrial and Engineering Chemistry)
권호 표지

광합성 미세조류 Nannochloropsis oculata의 최적배양 조건

Optimal Culture Conditions for Photosynthetic Microalgae Nannochloropsis oculata

  • 박현진 (신라대학교 공과대학 생명공학과) ;
  • 진은정 (신라대학교 공과대학 생명공학과) ;
  • 정태만 (에이엠 바이오(주)) ;
  • 주현 (인제대학교 생리학교실) ;
  • 이재화 (신라대학교 공과대학 생명공학과)
  • Park, Hyun-Jin (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Jin, Eun-Jung (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Jung, Tae-Man (Marine-Bio Center, Silla University) ;
  • Joo, Hyun (Department of Physiology and Integrated Biosystems, College of Medicine, Inje University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
  • 투고 : 2010.09.06
  • 심사 : 2010.09.28
  • 발행 : 2010.12.10
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초록

미세조류는 전 세계 바다에 분포하고 있으며 일부 종들은 인간의 식품에 이용되어 왔다. 특히, 광합성 미세조류 Nannochloropsis oculata는 영양적 가치가 우수하여 관심을 받고 있다. 본 연구에서는 광합성 미세조류 Nannochloropsis oculata의 고농도배양을 위한 배양온도, 초기 pH, 배양액 선정, 인공해수 농도, 배지농도, $CO_2$영향 등 최적조건을 확립하고자 하였다. 그 결과, 3%의 인공해수, 초기 pH 8.5, 배양온도 $25^{\circ}C$가 최적 배양조건으로 판별되었다. 미세조류에 $CO_2$를 공급하지 않았을 때에는 건조 균체량이 0.76 g/L이었지만, 5% $CO_2$ 공급 이후 1.50 g/L로 높은 성장률을 보였다. 클로로필 생합성은 미세조류 성장과 깊은 연관이 있는 것으로 판명되었다.

Microalgae has been seen all over the seawater and several species are used for human food. Specially, Nannochloropsis oculata, a photosynthetic microalgae, has been focused for a vast array of valuable nutritious compounds. In order to find high mass Nannochloropsis oculata culture conditions, some of important growth factors of pH, temperature, culture media, and $CO_2$ effect were tested. The optimal growth condition was found to be as follows : 3% artificial seawater, initial pH 8.5, and temperature $25^{\circ}C$. The alga mass and chlorophyll content were dramatically increased by applying 5% flue $CO_2$ gas (1.50 g/L algae in a continuous $CO_2$ flue; 0.76 g/L alga without $CO_2$). It was shown that the chlorophyll biosynthesis was also closely associated with alga growth.

키워드

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