Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Detection of Clostridium perfringens and its toxinotypes by enzyme linked immunosorbent assay from enterotoxaemic goats in Bangladesh

  • Islam, K.B.M.S. (Animal Health Research Division, Bangladesh Livestock Research Institute, and Laboratory of Microbial Physiology, Graduate School of Agriculture, Hokkaido University) ;
  • Rahman, M.S. (Department of Medicine, Bangladesh Agricultural University) ;
  • Ershaduzzaman, Md. (Senior Scientific Officer, Bangladesh Livestock Research Institute) ;
  • Taimur, M.J.F.A. (Senior Scientific Officer, Bangladesh Livestock Research Institute) ;
  • Jang, Hyung-Kwan (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine, and Korea Zoonoses Research Institute, Chonbuk National University) ;
  • Song, Hee-Jong (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine, and Korea Zoonoses Research Institute, Chonbuk National University)
  • Received : 2009.12.03
  • Accepted : 2010.02.26
  • Published : 2010.03.30
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Abstract

An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.

Keywords

References

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