Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Cloning and Characterization of Isoflavone synthase from Lespedeza bicolor

싸리에서 isoflavone synthase 유전자의 분리 및 생화학적 특성

  • Kim, Bong-Gyu (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
  • 김봉규 (건국대학교 생명공학과, 건국대학교 생명분자정보학 센터)
  • Received : 2010.03.02
  • Accepted : 2010.03.17
  • Published : 2010.03.31
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Abstract

None of genes from Lespedeza bicolor involved in isoflavonoid biosynthesis have been biochemically characterized. An isoflavone synthase from Lespedeza bicolor was cloned. To verify its catalytic activity, a fusion protein of LbIFS with P450 reductase from rice was made. Using this construct, production of isoflavone from flavanone was confirmed.

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References

  1. Akashi T, Aoki T, and Ayabe SI. (1999) Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice. Plant Physiol 121, 821-828. https://doi.org/10.1104/pp.121.3.821
  2. Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M, and Fukami Y. (1987) Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem 262, 5592-5595.
  3. Choi JN, Kim D, Choi HK, Yoo KM, Kim J, and Lee CH (2009) 2'-Hydroxylation of genistein enhanced antioxidant and antiproliferative activities in MCF-7 human breast cancer cells. J Microbiol Biotechnol 19, 1348-1354
  4. Ferguson JA and Mathesius U. (2003) Signaling interactions during nodule development. J Plant Growth Regul 22, 47-72. https://doi.org/10.1007/s00344-003-0032-9
  5. Forkmann G and Heller W (1999) Biosynthesis of flavonoids. In: Barton D, Nakanishi K, and Meth-Cohn O (eds.), Comprehensive Natural Products Chemistry. Elsevier Science Ltd., Oxford, pp. 713-748.
  6. Jung W, Yu O, Lau SM C, O'Keefe D P, Odell J, Fader G, and McGonigle B. (2000) Identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes. Nat Biotech 18, 208-212. https://doi.org/10.1038/72671
  7. Kim BG, Kim SY, Song HS, Lee C, Hur HG, Kim SI, and Ahn JH (2003) Cloning and expression of the isoflavone synthase gene (IFS-Tp) from Trifolium pretense. Mol Cells 15, 301-306.
  8. Kim DH, Kim BG, Jung NR, and Ahn JH (2009) Production of genistein from naringenin using Escherichia coli containing isoflavone synthase-cytochrome P450 reductase fusion protein. J Microbiol Biotechnol 19, 1612-1616. https://doi.org/10.4014/jmb.0905.05043
  9. Leonard E and Koffas MAG. (2007) Engineering of artificial plant cytochrome P450 enzymes for synthesis of isoflavones by Escherichia coli. Appl Environ Microbiol 73, 7246-7251. https://doi.org/10.1128/AEM.01411-07
  10. Maximov OB, Kulesh NI, Stepanenko LS, and Dmitrenok PS. (2004) New prenylated isoflavanones and other constituents of Lespedezabicolor. Fitoterapia 75, 96-98. https://doi.org/10.1016/j.fitote.2003.07.012
  11. Nebert DW and Gonzalez FJ (1987) P450 genes: structure, evolution, and regulation. Annu Rev Biochem 56, 945-993. https://doi.org/10.1146/annurev.bi.56.070187.004501
  12. Reynaud J, Guilet D, Terreux R, Lussignol M, and Walchshofer N. (2005) Isoflavonoids in non-leguminous families: an update. Nat Prod Rep 22, 504-515. https://doi.org/10.1039/b416248j
  13. Tahara S. (2007) A journey of twenty-five years through the ecological biochemistry of flavonoids. Biosci Biotechnol Biochem 71, 1387-1404. https://doi.org/10.1271/bbb.70028
  14. Veitch NC. (2007) Isoflavonoids of the leguminosae. Nat Prod Rep 24, 4170464.
  15. Winkel-Shirley B. (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology and biotechnology. Plant Physiol 126, 485-493. https://doi.org/10.1104/pp.126.2.485