Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Insecticidal activity of the crude extract and its fractions of Custard apple (Annona reticulata L.)

커스타드애플(Annona reticulata L.)씨 추출물과 그 분획물의 살충활성

  • Shin, Suk-Hyun (Chemical Safety Division, National Academy of Agricultural Sciences, RDA) ;
  • Choi, Geun-Hyoung (Chemical Safety Division, National Academy of Agricultural Sciences, RDA) ;
  • Choi, Dal-Sun (Chemical Safety Division, National Academy of Agricultural Sciences, RDA) ;
  • Kwon, Oh-Kyung (Chemical Safety Division, National Academy of Agricultural Sciences, RDA) ;
  • Im, Geon-Jae (Chemical Safety Division, National Academy of Agricultural Sciences, RDA) ;
  • Park, Jae-Up (Chemical Safety Division, National Academy of Agricultural Sciences, RDA) ;
  • Choi, Byung-Ryul (Applied Entomology Division, National Academy of Agricultural Sciences, RDA) ;
  • Kim, Tae-Wan (School of Plant Life Environmental & Science, Hankyung National University) ;
  • Kim, Jin-Hyo (Chemical Safety Division, National Academy of Agricultural Sciences, RDA)
  • 신숙현 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 최근형 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 최달순 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 권오경 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 임건재 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 박재읍 (농촌진흥청 국립농업과학원 유해화학과) ;
  • 최병렬 (국립농업과학원 곤충산업과) ;
  • 김태완 (한경대학교 식물생명자원과학부) ;
  • 김진효 (농촌진흥청 국립농업과학원 유해화학과)
  • Received : 2009.09.24
  • Accepted : 2009.12.29
  • Published : 2010.03.31
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Abstract

In recent years, many agricultural scientists are studying on eco-friendly farming methods. Among of lots of the methods, the natural insecticides are highly motivated and interested due to their safety and biodegradable issues, and readily available source of bioinsecticides. In this study, the crude extract of custard apple (Annona reticulata L.) seed and its three fractions which were separated based on polarity indexes were examined for their insecticidal activities against Myzus persicae Sulzer and Nilaparvata lugens S.. The crude extract (Aceton/MeOH) showed strong insecticidal activities against both insects at 3.00 mg/mL ($LD_{50}$=0.45mg/mL for M. persicae S. and 1.42 mg/mL for N. lugens S.). Furthermore, simple fractionation with hexane, chloroform, and water lead to increase three-folds insecticidal activity on chloroform fraction ($LD_{50}$=0.13mg/mL for M. persicae S. and 1.14 mg/mL for N. lugens S.). The results suggest that A. reticulata extracts might be used to control for M. persicae effectively.

커스타드애플(Annona reticulata L.)씨 추출물 및 그의 분획물을 활용하여 복숭아혹진딧물 및 벼멸구를 대상으로 살충활성을 조사하였으며, 그 결과 아세톤/메탄올 조추출물 $3000\;{\mu}g/mL$에서 복숭아혹진딧물은 100%, 벼멸구에 대해서는 92%의 살충률을 나타내었다. 이들에 대한 $LD_{50}$는 각각 $446.3\;{\mu}g/mL$와 $1423.6\;{\mu}g/mL$로 복숭아혹진딧물이 추출물에 높은 감수성을 나타내었다. 이들의 핵산, 클로로포름, 물 분획물에 대한 분리정제물의 경우 클로로포름 분획물이 조추출물 분획물보다 약 3배 높은 살충능이 관찰되었으며, 이는 극성분배를 통해 살충성이 높은 물질군을 선별적으로 정제할 수 있음을 보여준다. 특히 클로로포름 분획물에 대한 살충능의 경우 $137.2\;{\mu}g/mL$(복숭아혹진딧물), $1145.5\;{\mu}g/mL$(벼멸구)의 농도에서 $LD_{50}$가 관찰되었다.

Keywords

References

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