Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidant Activity of Branches of Fuyu and Nishimurawase Persimmon Trees

부유 및 서촌조생 감나무 가지의 항산화 활성

  • You, Dong-Hyun (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Jang, In-Cheol (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Seo, Go-Un (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Su-Jung (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Yang, Eun-Kyung (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Ahn, Gwang-Hwan (Sweet Persimmon Research Institute, Gyungsangnamdo Agricultural Research & Extension Services) ;
  • Matsushita, Yasuyuki (Graduate School of Bioagricultural Sciences, Nagoya University) ;
  • Fukushima, Kazuhiko (Graduate School of Bioagricultural Sciences, Nagoya University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University)
  • Received : 2009.12.21
  • Accepted : 2010.05.12
  • Published : 2010.06.30
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Abstract

The antioxidant activities of two persimmon (Diospyros kaki T.) branches were evaluated. After preparing acetone, 70% acetone, and methanol extracts from Fuyu and Nishimurawase persimmon branches, DPPH radical scavenging activity (RSA), ABTS RSA, reducing power (RP), and total phenol contents (TPC) were determined. The acetone extracts of Fuyu and Nishimurawase showed higher antioxidant properties than the other extracts. The highest TPC (22.48 mg/g), DPPH RSA ($IC_{50}=701.48\;{\mu}g/mL$), ABTS RSA ($IC_{50}=493.76\;{\mu}g/mL$), and RP ($IC_{50}=1218.57\;{\mu}g/mL$) were found in acetone extract of Fuyu, while the lowest antioxidant activities were in 70% acetone extract of Nishimurawase. The results suggest that sweet persimmon branches exhibit considerable antioxidant activity.

경남 진영에서 재배되고 있는 부유와 서촌조생 품종의 감나무 가지를 각각 메탄올, 아세톤, 70% 아세톤으로 추출한 후 항산화 활성을 조사하였다. 총 페놀 함량의 경우, 부유 감나무 가지의 아세톤 추출물이 620.74 mM GAE로써 가장 높았고, 서촌 조생 감나무 가지의 아세톤 추출물은 525.91 mM GAE이었다. 항산화 활성은 DPPH 라디칼 소거능, ABTS 라디칼 소거능, 환원력으로 측정하였다. DPPH 라디칼 소거능, ABTS 라디칼 소거능, 환원력 모두 부유 감나무 가지의 아세톤 추출물에서 가장 높은 활성을 보였으며, 메탄올과 70% 아세톤 추출물 순으로 높은 활성을 나타내었다. 이상의 결과로 부유 감나무 가지가 서촌조생 감나무 가지에 비해 높은 항산화 활성을 가지며, 용매별로는 아세톤 추출물이 높은 항산화 활성을 가지는 것을 알 수 있었다. 이를 통해 단감 나뭇가지가 천연 항산화제로서 가능성을 가지고 있음을 확인할 수 있었다.

Keywords

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