The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Proteomic Analysis of Differentially Expressed Proteins in Bovine Endometrium with Endometritis

  • Choe, Chang-Yong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Park, Jeong-Won (Department of Microbiology, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Kim, Eun-Suk (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Lee, Sung-Gyu (Department of Microbiology, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Park, Sun-Young (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Lee, Jeong-Soon (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Cho, Myung-Je (Department of Microbiology, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Kang, Kee-Ryeon (Department of Biochemistry, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Han, Jae-Hee (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Kang, Da-Won (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine)
  • Received : 2010.06.03
  • Accepted : 2010.08.05
  • Published : 2010.08.30
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Abstract

Endometritis is one of the primary reasons for reproductive failure. In order to investigate endometritis-associated marker proteins, proteomic analysis was performed on bovine endometrium with endometritis. In bovine endometritis, desmin, $\alpha$-actin-2, heat-shock protein (HSP) 27, peroxiredoxin-6, luteinizing hormone receptor isoform 1, collectin-43 precursor, deoxyribonuclease-I (DNase-I), and MHC class I heavy chain (MHC-Ih) were up-regulated. In contrast, transferrin, interleukin-2 precursor, hemoglobin $\beta$ subunit, and potassium channel tetramerisation domaincontaining 11 (KCTD11) were down-regulated in comparison to normal endometrium. The proteomic results were validated by semiquantitative-PCR and immunoblot analysis. The mRNA levels of desmin, transferrin, $\alpha$-actin-2, HSP27, KCTD11, and MHC-Ih were up-regulated by over 1.5-fold, and showed a pattern similar to their proteomic profiles. Desmin and $\alpha$-actin-2 protein showed positive correlations between proteomic analysis and immunoblot analysis. These results suggest that desmin and $\alpha$-actin-2 may play important roles in endometritis-related function, and could be useful markers for the diagnosis of bovine endometritis.

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