생명과학회지 (Journal of Life Science)

  • 제20권10호
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  • Pages.1498-1504
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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한우(Bos taurus coreanae)의 CAST 유전자 내 변이지역 탐색 및 경제형질과의 연관성 분석

Identification of Polymorphisms in CAST Gene Associated with Economic Traits in Hanwoo (Bos taurus coreanae)

  • 오재돈 (한경대학교 유전정보연구소) ;
  • 이진아 (한경대학교 유전정보연구소) ;
  • 이건우 (한경대학교 유전정보연구소) ;
  • 박경도 (한경대학교 유전정보연구소) ;
  • 조병욱 (부산대학교 생명자원과학대학) ;
  • 전광주 (한경대학교 유전정보연구소) ;
  • 이학교 (한경대학교 유전정보연구소) ;
  • 공홍식 (한경대학교 유전정보연구소)
  • Oh, Jae-Don (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Jin-A (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Kun-Woo (Genomic Informatics Center, Hankyong National University) ;
  • Park, Kyung-Do (Genomic Informatics Center, Hankyong National University) ;
  • Cho, Byung-Wook (School of Bio-Resources, Pusan National University) ;
  • Jeon, Gwang-Joo (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Hak-Kyo (Genomic Informatics Center, Hankyong National University) ;
  • Kong, Hong-Sik (Genomic Informatics Center, Hankyong National University)
  • 투고 : 2010.07.08
  • 심사 : 2010.10.18
  • 발행 : 2010.10.30
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초록

Calpastatin (CAST)은 calpain의 억제제 역할을 하는 유전자이며 BTA7에 위치하고 있다. Calpain을 억제하는 CAST 유전자 내 다형성이 육질의 연도에 영향을 미치고 있는 것이 알려져 있다. 또한 여러 연구를 통해 calpain 시스템은 일반 골격근의 성장에 중요한 역할을 하는 것으로 보고되었다. 따라서 본 연구는 한우 집단의 CAST 유전자 내 변이 지역을 탐색하여 경제형질과의 연관성을 분석하기 위하여 실시하였다. CAST 유전자(Accession no. NC_007305) 단일염기변이지역을 탐색 결과 총 5개의 변이(109749T/C, 116151G/A, 109737G/A, 109823T/C, 109926G/A) 지역이 탐색 NCBI에 등록되어 있지 않은 새로운 변이로 본 연구를 통해 탐색되었다. 한우집단 내에서 탐색된 총 5개의 SNP를 대상으로 연관불균형(LD, Linkage disequilibrium) 분석을 수행하였다. 변이들 간의 연관불균형(LD) 정도가 가장 낮은 수준에 있는 2개의 변이지역(109926G/A와 116151G/A, $r^2$=0.038)을 대상으로 경제형질과의 연관성 분석을 실시한 결과 등심단면적(109926G/A, p<0.05)과 18개월령 체중(116151G/A, p<0.05)에서 유의적인 연관성이 검출 되었다. CAST유전자는 도축 후 숙성과정에서 고기의 연도를 증가시키는데 영향을 미칠 뿐만 아니라 가축의 성장에도 깊은 연관성이 있으며, 본 연구를 통해 CAST 유전자 내 변이는 성장 관련 형질과의 연관성이 유의적인 것으로 확인되었다.

A number of studies have shown that the calpain system is important in normal skeletal muscle growth. An increased rate of skeletal muscle growth can result from a decreased rate of muscle protein degradation, and this is associated with a decrease in activity of the calpain system, due principally to a large increase in calpastatin (CAST) activity. The CAST gene, mapped to BTA 7, is considered a candidate gene for beef tenderness and muscle growth. The present study used comparative sequencing of five novel polymorphisms located within exon 20 and 22 of the bovine CAST gene in Hanwoo: exon20- 109737G/A, 109749T/C, 109823T/C, exon22- 116151G/A, intron- 109926G/A. The association of the CAST SNPs with economic traits was studied. The 109926G/A showed a significant effect only on the longissimus muscle area (LMA, p<0.05) in Hanwoo. 109926G/A with the genotype GG had a significantly higher effect on LMA (75.35) than the genotype AA (69.6, p<0.05). Also, the 116151G/A showed a significant effect only on weight at 18 months (W18, p<0.05). 116151G/A with the genotype GG had a significantly higher effect on W18 (428.54) than the genotype AA (408.87, p<0.05).

키워드

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피인용 문헌

  1. Identification of single nucleotide polymorphisms in the ACADS gene and their relationships with economic traits in Hanwoo vol.39, pp.2, 2012, https://doi.org/10.7744/cnujas.2012.39.2.219
  2. Association of CAST gene polymorphisms with carcass and meat quality traits in Yanbian cattle of China vol.40, pp.2, 2013, https://doi.org/10.1007/s11033-012-2243-2
  3. Identification of a SNP in Cattle HGD Gene with its Effect on Economic Trait in Hanwoo vol.24, pp.11, 2014, https://doi.org/10.5352/JLS.2014.24.11.1168