Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Cadmium Exposure on Tissues of Carassius auratus

카드뮴 노출이 붕어(Carassius auratus) 조직에 미치는 영향

  • Shin, Myung-Ja (Department of Biological Science, Andong National University) ;
  • Kwon, O-Chang (Department of Biological Science, Andong National University) ;
  • Lee, Jong-Eun (Department of Biological Science, Andong National University) ;
  • Seo, Eul-Won (Department of Biological Science, Andong National University)
  • 신명자 (안동대학교 자연과학대학 생명과학과) ;
  • 권오창 (안동대학교 자연과학대학 생명과학과) ;
  • 이종은 (안동대학교 자연과학대학 생명과학과) ;
  • 서을원 (안동대학교 자연과학대학 생명과학과)
  • Received : 2010.07.07
  • Accepted : 2010.09.16
  • Published : 2010.10.30
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Abstract

The present study aimed to investigate the level of accumulated heavy metal in various tissues of Carassius auratus after exposure to Cadmium (Cd), histologically and physiologically. After treating C. auratus with Cd, the accumulated Cd in gill tissues was detected to be of the highest content, and showed the lowest content in integument tissues. Also, Cd content increased in a time dependent manner and showed the highest accumulation in the tissues exposed for 20 days. Antioxidant enzyme activities showedhigher activity in the gill and integument than in the kidney and liver tissues. In the case of SOD, antioxidation activity of SOD in all Cd exposed tissues was higher than in unexposed tissues. The activities of SOD and CAT also became higher after Cd exposure. Gill tissues exposed to Cd showed an increased number of mucous cells between lamella in a time dependent manner. In addition, the gills showed morphological changes such as edema, exfoliation of epithelial cells, and fusion of the secondary lamellae. Also, exposure to Cd for 20 days had an effect on gill tissues, causing membrane damage in the mitochondria and nucleus. In kidney tissues, atrophied glomerulus was observed, and the empty space in Bowman's capsule was wider.

본 연구는 Cd에 노출시킨 붕어를 통하여 각 조직에 축적된 Cd 양과 항산화효소 활성을 통해 생리적인 변화 및 아가미와 신장 조직에서의 미세구조 변화를 조사하였다. Cd을 처리한 실험군 붕어 조직 내 Cd의 함량은 아가미 조직에서 가장 높았고 근육 조직에서 가장 낮았다. 또한 모든 조직에서 Cd의 함량은 노출기간이 길어질수록 증가하여 노출 20일에 가장 높았다. 항산화효소의 활성은 아가미와 근육 조직에서 높았으며, 신장과 간 조직에서 낮았다. 모든 조직에서 SOD의 활성이 가장 높았으며, SOD와 CAT의 활성은 10일에 높은 활성을 보이고 감소하는 유사한 결과를 보였다. Cd에 20일간 노출시킨 붕어의 아가미 조직은 대조군 아가미 조직에 비하여 노출기간이 길어질수록 새변 사이에 점액세포의 수가 증가하였으며, 이차새변에서는 부종, 상피세포의 박리와 새변과 새변이 융합한 형태도 관찰되었다. 또한 아가미 조직의 미토콘드리아와 핵에서는 막의 손상이 확인되었다. 신장 조직은 대조군에서 보우만 주머니 안에 사구체가 꽉 찬 정상구조였으나 노출기간이 길어짐에 따라 사구체가 수축하여 보우만 주머니 안의 공간이 넓은 형태로 관찰되었다.

Keywords

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