Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Pilot-scale Optimization of Parameters Related to Dissolved Oxygen for Mass Production of Pullulan by Aureobasidium pullulans HP-2001

Aureobasidium pullulans HP-2001 균주를 사용한 풀루란의 대량 생산을 위한 파이롯트 규모에서 용존산소와 관련된 조건의 최적화

  • Gao, Wa (Department of Medical Bioscience, Graduate School of Donga-A University) ;
  • Kim, Yi-Joon (Department of Medical Bioscience, Graduate School of Donga-A University) ;
  • Chung, Chung-Han (BK21 Bio-Silver Project of Dong-A University) ;
  • Li, Jianhong (College of Plant Science & Technology, Huazhong Agricultural University) ;
  • Lee, Jin-Woo (BK21 Bio-Silver Project of Dong-A University)
  • 고와 (동아대학교 대학원 의생명과학과) ;
  • 김이준 (동아대학교 대학원 의생명과학과) ;
  • 정정한 (동아대학 BK21 생물자원 실버바이오사업 인력양성단) ;
  • 이잔홍 (중국 화중농업대학교 식물과학기술대학) ;
  • 이진우 (동아대학 BK21 생물자원 실버바이오사업 인력양성단)
  • Received : 2010.07.26
  • Accepted : 2010.10.12
  • Published : 2010.10.30
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Abstract

Parameters related to dissolved oxygen for the production of pullulan by Aureobasidium pullulans HP-2001 were optimized in 7 l and 100 l bioreactors. The optimal concentrations of glucose and yeast extract for the production of pullulan were 50.0 and 2.5 g/l, respectively, and its conversion rate from glucose was 37% at a flask scale. The optimal initial pH of the medium and temperature for cell growth were 7.5 and $30^{\circ}C$, whereas those for the production of pullulan were 6.0 and $25^{\circ}C$. The optimal agitation speed and aeration rate for cell growth were 600 rpm and 2.0 vvm in a 7 l bioreactor, whereas those for the production of pullulan were 500 rpm and 1.0 vvm. The production of pullulan with an optimized agitation speed of 500 rpm and aeration rate of 1.0 vvm was 18.13 g/l in a 7 l bioreactor. Maximal cell growth occurred without inner pressure, whereas the optimal inner pressure for the production of pullulan was 0.4 kgf/$cm^2$ in a 100 l bioreactor. The production of pullulan under optimized conditions in this study was 22.89 g/l in a 100 l bioreactor, which was 1.38 times higher than that without inner pressure.

Aureobasidium pullulans HP-2001 균주를 사용하여 풀루란을 대량 생산을 위하여 7 l 및 100 l 생물배양기를 사용하여 용존산소와 관련된 조건을 최적화하였다. 풀루란의 생산에 최적인 탄소원과 질소원은 각각 50.0 g/l 포도당 및 2.5 g/l 효모추출물이었으며 플라스크 규모에서의 풀루란 변환율은 37%이었다. 풀루란 생산 균주의 생장에 최적인 배지의 초기 pH 및 배양온도는 7.5 및 30oC이었으나 풀루란의 생산에 최적인 배지의 초기 pH 및 배양 온도는 각각 6.0 및 $25^{\circ}C$이었다. 7 l 생물배양기에서 Aureobasidium pullulans HP-2001 균주의 생육에 최적인 교반속도 및 통기량은 각각 600 rpm 및 2.0 vvm이었으나 풀루란 생산에 최적인 조건은 각각 500 rpm 및 1.0 vvm이었으며 최적 조건에서 풀루란의 생산농도는 18.13 g/l이었다. 100 l 생물배양기에서 풀루란 생산 균주의 생장에 최적인 내압은 0.0 kgf/$cm^2$이었으나, 풀루란 생산에 최적인 내압은 0.4 kgf/$cm^2$이었으며 최적 조건에서 풀루란의 생산 농도는 22.89 g/l이었다. 이는 내압이 없는 상태에 비하여 풀루란의 생산 농도가 1.38배 증가한 것이다.

Keywords

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