Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Validation of the Analysis of Domoic Acid using High Performance Liquid Chromatography

HPLC에 의한 Domoic acid 분석법 검정

  • Mok, Jong-Soo (Food & Safety Research Division, National Fisheries Research & Development Institute) ;
  • Lee, Ka-Jeong (Food & Safety Research Division, National Fisheries Research & Development Institute) ;
  • Song, Ki-Cheol (Food & Safety Research Division, National Fisheries Research & Development Institute) ;
  • Kim, Ji-Hoe (Southeast Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
  • 목종수 (국립수산과학원 식품안전과) ;
  • 이가정 (국립수산과학원 식품안전과) ;
  • 송기철 (국립수산과학원 식품안전과) ;
  • 김지회 (국립수산과학원 남동해수산연구소)
  • Received : 2010.06.25
  • Accepted : 2010.08.12
  • Published : 2010.08.31
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Abstract

To prevent amnesic shellfish poisoning (ASP) resulting from the consumption of shellfish contaminated with domoic acid, the quantitative analysis of domoic acid is very important. We validated a high performance liquid chromatography (HPLC) method for accurate and precise quantification of domoic acid. A clear peak and the isolation of domoic acid resulted on injecting a 50% methanol extract of CRM-ASP-Mus-c mussel reference material using HPLC. The limit of detection of domoic acid under the established HPLC conditions was $0.10\;{\mu}g/g$, and the limit of quantification of the toxin under the same conditions was $0.25\;{\mu}g/g$. The intra-accuracy and precision for domoic acid in CRM-ASP-Mus-c were 90.7-95.7% and 0.28-22.25%, respectively. The inter-accuracy and precision for domoic acid were 89.1-97.1% and 1.7-4.1%, respectively. The mean recovery of domoic acid in methanol extracts from ten species of marine invertebrates was 88.6-1105.1%.

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