The Journal of Korean Physical Therapy

  • 제22권1호
  • /
  • Pages.67-73
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  • 2010
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  • 1229-0475(pISSN)
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  • 2287-156X(eISSN)
대한물리치료학회 (The Korean Society of Physical Therapy)
권호 표지

줄기세포를 이식한 척수손상 흰쥐에서 반복자기자극의 효과

The Effect of Repetitive Magnetic Stimulation in an SCI Rat Model with Stem Cell Transplantation

  • 배영경 (영남대학교 의과대학 병리학교실) ;
  • 박해운 (대구가톨릭대학교 의과대학 재활의학교실) ;
  • 조윤우 (영남대학교 의과대학 재활의학교실) ;
  • 김수정 (영남대학교 의과학연구소) ;
  • 이준하 (영남대학교 생화학분자생물학교실) ;
  • 권정구 (대구가톨릭대학교 의과대학 재활의학교실) ;
  • 안상호 (영남대학교 의과대학 병리학교실)
  • Bae, Young-Kyung (Department of Pathology, College of Medicine, Yeungnam University) ;
  • Park, Hea-Woon (Department of Rehabilitation Medicine, School of Medicine, Catholic University of Deagu) ;
  • Cho, Yun-Woo (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Kim, Su-Jeong (Institute of Medical Science, Yeungnam University) ;
  • Lee, Joon-Ha (Department of Biochemistry and Molecular Biology, YeungnamUniversity) ;
  • Kwon, Jung-Gu (Department of Rehabilitation Medicine, School of Medicine, Catholic University of Deagu) ;
  • Ahn, Sang-Ho (Department of Pathology, College of Medicine, Yeungnam University)
  • 투고 : 2010.01.11
  • 심사 : 2010.02.12
  • 발행 : 2010.02.25
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초록

Purpose: We tested whether repetitive transcranial magnetic stimulation (rTMS) improved recovery following spinal cord injury (SCI) in rats with transplantation of adipose tissue-derived stromal cells (ATSCs). Methods: Twenty Sprague-Dawley rats (200-250 g, female) were used. Moderate spinal cord injury was induced at the T9 level by a New York University (NYU) impactor. The rat ATSCs (approximately $5{\times}10^5$ cells) were injected into the perilesional area at 9 days after SCI. Starting four days after transplantation, rTMS (25 Hz, 0.1 Tesla, pulse width=$370{\mu}s$, on/off time=3 sec/3 sec) was applied daily for 7 weeks. Functional recovery was assessed using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale as well as pain responses for thermal and cold stimuli. Results: Both groups showed similar, gradual improvement of locomotor function. rTMS stimulation decreased thermal and cold hyperalgesia after 7 weeks, but sham stimulation did not. Conclusion: rTMS after transplantation of ATSCs in an SCI model may reduce thermal hyperalgesia and cold allodynia, and may be an adjuvant therapeutic tool for pain control after stem cell therapy in SCI.

키워드

참고문헌

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