Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Comparison of Hematological Properties in Cultured Olive Flounder Paralichthys olivaceus on Different Growth Stages and Seasons

양식넙치 Paralichthys olivaceus의 성장단계별, 계절별 혈액학적 성상비교

  • Kim, Won-Jin (Dept. of Marine Bio-materials and Aquaculture, Pukyong National University) ;
  • Kim, Young-Soo (Dept. of Marine Bio-materials and Aquaculture, Pukyong National University) ;
  • Chang, Young-Jin (Dept. of Marine Bio-materials and Aquaculture, Pukyong National University)
  • 김원진 (부경대학교 해양바이오신소재학과) ;
  • 김영수 (부경대학교 해양바이오신소재학과) ;
  • 장영진 (부경대학교 해양바이오신소재학과)
  • Received : 2010.03.28
  • Accepted : 2010.05.11
  • Published : 2010.06.30
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Abstract

This study was conducted to examine the hematological factors in cultured olive flounder Paralichthys olivaceus depending on its growth stage and season. The study also aims at developing the standard hematological indicator for growth stage and season by examining total 16 parameters including whole blood (hematocrit, red blood cell and hemoglobin), biochemical (glucose, cholesterol, total protein, AST, ALT, $Na^+,\;K^+,\;Cl^-,\;Ca^{2+},\;Mg^{2+}$ and osmolarity), and endocrine (cortisol and $T_3$) factors in plasma of cultured olive flounder. The result showed a growth stage-dependent increase of $T_3$ level in olive flounder while the level of cholesterol showed an inverse correlation to fish size. For seasonal fluctuation in cultured olive flounder of the same growth stage, the highest level of Ht and RBC was observed in autumn.

이 연구는 양식산 넙치의 성장단계별 및 계절에 따른 생리학적 반응과 혈액학적 요소들을 파악하고자 하였으며, 분석된 항목으로는 hematocrit, red blood cell, hemoglobin, 글루코스, 콜레스테롤, 총단백질, AST, ALT, $Na^+,\;K^+,\;Cl^-,\;Ca^{2+},\;Mg^{2+}$, osmolality, 코티졸 및 $T_3$였다. 성장단계별 혈액학적 성상에 있어 $T_3$는 어류의 크기가 클수록 증가하는 경향을 나타내었다. 반면, 콜레스테롤은 어체의 크기가 클수록 감소하는 경향을 보였다. 계절별 혈액학적 성상에 있어 Ht와 RBC는 가을에 가장 높은 수준을 나타냈다. 코티졸은 모든 그룹에서 $2.2{\pm}0.4{\sim}4.3{\pm}1.9ng/m{\ell}$의 농도를 보였다. 나머지 혈액학적 요소는 성장단계별, 계절별로 특이한 차이를 나타내지 않았다. 본 연구에서 분석 비교한 각종 혈액학적 요소의 측정값은 양식산 넙치의 생리활성 평가에 활용될 수 있을 것으로 기대된다.

Keywords

References

  1. Ackerman PA, Forsyth RB, Mazur CF, Iwama GK (2000) Stress hormones and the cellular stress response in salmonids. Fish Physiol Biochem 23:327-336. https://doi.org/10.1023/A:1011107610971
  2. Barton BA, Iwama GK (1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Ann Rev Fish Dis 1:3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  3. Casillas E, Ames W (1985) Serum chemistry of diseased English sole, Parophrys vetulus Girard, from polluted areas of Puget Sound, Washington. J Fish Dis 8:437-449. https://doi.org/10.1111/j.1365-2761.1985.tb01277.x
  4. Cataldi E, Marco PD, Mandich A, Cataudella S (1998) Serum parameters of Adriatic sturgeon Acipenser naccarii (Pisces: Acipen-seriformes): effects of temperature and stress. Comp Biochem Physiol Part A 121:351-354. https://doi.org/10.1016/S1095-6433(98)10134-4
  5. Chang YJ, Lee YC, Lee BK (1996) Comparison of growth and survival rates of juvenile grey mullets (Mugil cephalus) in different salinities. J Aquacult 9:311-320.
  6. Chang YJ, Hur JW (1999) Physiological responses of grey mullet (Mugil cephalus) and nile tilapia (Oreochromis niloticus) by rapid changes in salinity of rearing water. J Kor Fish Soc 32:310-316.
  7. Chang YJ, Park MR, Kang DY, Lee BK (1999) Physiological responses of cultured olive flounder (Paralichthys olivaceus) on series of lowering seawater temperature sharply and continuously. J Kor Fish Soc 32:601-606.
  8. Chang YJ, Hur JW, Lim HK, Lee JK (2001) Stress in olive fllounder (Paralichthys olivaceus) and fat cod (Hexagrammos otakii) by the sudden drop and rise of water temperature. J Kor Fish Soc 34:91-97.
  9. Chang YJ, Min BH, Chang HJ, Hur JW (2002) Comparison of blood physiology in juvenile black seabream (Acanthopagrus schlegeli) reared in converted freshwater from seawater and seawater from freshwater. J Kor Fish Soc 35:595-600.
  10. Davis KB, Parker NC (1990) Physiological stress in striped bass: Effect of acclimation temperature. Aquaculture 91:349-358. https://doi.org/10.1016/0044-8486(90)90199-W
  11. Donaldson EM (1981) The Pituitary-Interrenal Axis as an Indicator of Stress in Fish. pp 11-47 in Pickering AD.
  12. Garrido MA, Perez P, Titus JA, Valdayo MJ, Winkler DF, Barbieri SA, Wunderlich JR, Segal DM (1990) Targeted cytotoxic cells in human peripheral blood lymphocytes. J Immunol 144:2891-2898.
  13. Gordon RB (1968) Distribution of transaminases (Aminotransferases) in the tissue of the Pacific salmon (Oncorhynchus) with emohasis on the properties and diagnostic use of glutamic oxaloacetic transaminase. J Fish Res Bd Can 25:1247-1268. https://doi.org/10.1139/f68-108
  14. Higashimoto Y, Nakao N, Ohkubo T, Tanaka M, Nakashima K (2001) Structure and tissue distribution of prolactin receptor mRNA in Japanese flounder (Paralichthys olivaceus): conserved and preferential expression in osmoregulatory organ. Gen Comp Endocrinol 123:170-179. https://doi.org/10.1006/gcen.2001.7660
  15. Hodson PV, McWhirter M, Ralph K, Gray B, Thivierge D, Carey JH, Van-Der-Kraak G, Whittle DM, Levesque MC (1992) Effects of bleached kraft mill effuent on fish in the St. Maurice River, Quebec. Environ Toxicol Chem 11:1635-1651. https://doi.org/10.1002/etc.5620111113
  16. Hur JW, Chang YJ, Lim HK, Lee BK (2001) Stress responses of cultured fishs elicited by water level reduction in rearing tank and fish transference during selection process. J Kor Fish Soc 34:465-471.
  17. Hur JW (2002) Physiological responses of fishes to the artificial stresses in the process of aquaculture. Ph.D. Thesis, Pukyong National University, Busan, Korea.
  18. Kang JC, Jee JH, Song SY, Moon SW (2004) Effects of oral administration with fermented product from sewage in land-based seawater fish farm on haematological factors of olive flounder, Paralichthys olivaceus. J Fish Pathol 17:57-66.
  19. Kim JH, Hur JW, Park IS, Kang HK, Chang YJ (2005) Effect of different anesthetic doses of MS-222 and lidocaine-HCl on the blood physiolosical responses in black rockfish, Sebastes schlegeli. J Aquacult 18:36-244.
  20. Leatherland JF, Sonstegard RA (1981) Effect of dietary mirex and PCBs on calcium and magnesium metabolism in rainbow trout, Salmo gairdneri and coho salmon, Oncorhynchus kisutch : A comparison with great lakes coho salmon. Comp Biochem Physiol C 69:45-351. https://doi.org/10.1016/0306-4492(81)90100-3
  21. Lee S, Lee JH, Kim K (2003) Effect of dietary essential fatty acids on growth, body composition and blood chemistry of juvenile starry flounder (Platichthys stellatus). Aquaculture 225:69-281.
  22. Logue J, Tiku P, Cossins AR (1995) Heat injury and resistance adaptation in fish. J Ther Biol 20:91-197. https://doi.org/10.1016/0306-4565(94)00031-D
  23. Ma G, Lin H, Zhang W (1995) Effects of cadmium on serum gonadotropin and growth hormone in common carp (Cyprinus carpio L.). J Fish China Shuichan Xuebao 19:20-126.
  24. Mater KS, Mayer FL, Witt A (1985) Waste transformer oil and PCB toxicity to rainbow trout. Am Fish Soc 114:69-886.
  25. MIFAFF (2009) Statistic Database for Fisheries Production. Retrieved from http://www.fips.go.kr on Oct 31.
  26. Min BH, Noh GA, Jeong MH, Kang DY, Choi CY, Bang IC, Chang YJ (2006) Effect of oral administration of thyroid hormone on physiological activity and growth of black porgy reared in freshwater or seawater. J Aquacult. 19:49-156.
  27. Munck A, Guyre PM, Holbrook J (1984) Physiological functions of glucocorticoid in stress and their relationship to pharmacological action. Endocrinol Rev 5:5-34.
  28. Myeong JI, Pack SY, Chang YJ (1997) Effect of water temperature and feeding rate on growth and feed efficiency of Korean rockfish, Sebastes schlegeli. J Aquacult 10:11-320.
  29. Park MR, Chang YJ, Kang DY (1999) Physiological response of the cultured olive flounder (Paralichthys olivaceus) to the sharp changes of water temperature. J Aquacult 12:21-228.
  30. Perry SF, Reid SD (1993) $\beta$-adrenergic signal transduction in fish : interactive effects of catecholamines and cortisol. Fish Physiol Biochem 11:95-203.
  31. Rao PP, Joseph KV, Rao KJ (1990) Histopathological and biochemical change in the liver of a fresh water fish exposed to heptachlor. J Nat Conserv 2:33-137.
  32. Sadler J, Wells RMG, Pankhurst PM, Pankhurst NW (2000) Blood oxygen transport, rheology and haematological responses to confinement stress in diploid and triploid Atlantic salmon, Salmo salar. Aquaculture 184:49-361.
  33. Sampaio LA, Bianchini A (2002) Salinity effects on osmoregulation and growth of the euryhaline flounder Paralichthys orbignyanus. J Exp Mar Biol Ecol 269: 187-196. https://doi.org/10.1016/S0022-0981(01)00395-1
  34. Singh H, Singh TP (1997) Formothion and propoxur induced ionic imbalance and skeletal deformity in a catfish, Heteropneustes fossilis. J Environ Biol 18:357-363.
  35. Smart GR (1981) aspects of water quality producing stress in intensive fish culture. pp. 277-293 (in) Stress and Fish, (ed) A.D. Pickering, Academic Press, London.
  36. Specker CB, Bradford CS, Fitzpatrick MS, Patino R (1989) Regulation of the interrenal of fishes: Non-classical control mechanism. Fish Physiol Biochem 7:259-265. https://doi.org/10.1007/BF00004715
  37. Thomas P, Robertson L (1991) Plasma cortisol and gliucose stress responses of red drum (Sciaenops ocellatus) to handling and shallow water stressors and anesthesia with MS-222, quinaldine sulfate and metomidate. Aquaculture 96:69-86. https://doi.org/10.1016/0044-8486(91)90140-3
  38. Tomasso JR, Simco BA, Davis KB (1983) Circulating corticosteroid and leucocyte dynamics in channel catfish during net confinement. Texas J Sci 35:83-88.
  39. Wedemeyer GA, McLeay DJ (1981) Methods for determining the tolerance of fishes to environmental stressors. In Stress and Fish (Ed. by A.D. Pickering), Academic Press London 247-275.
  40. Yamawaki K, Hashimoto W, Fujii K, Koyama J, Ikeda Y, Ozaki H (1986) Hemochemical changes in carp exposed to low cadmium concentrations. Bull Jap Soc Sci Fish 52:459-466. https://doi.org/10.2331/suisan.52.459
  41. Yanagisawa T, Hashimoto K (1984) Plasma albumins in elasmobranchs. Bull Jap Soc Sci Fish 1083.