Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
권호 표지

Fabrication of Fibroin Microspheres and Hollow Spheres

피브로인 미립구 및 중공미립구의 제조

  • Park, Cheol-Wan (Department of Bioengineering and Technology, Kangwon National University) ;
  • Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University) ;
  • Hur, Won (Department of Bioengineering and Technology, Kangwon National University)
  • 박철완 (강원대학교 공과대학 생물공학과) ;
  • 이신영 (강원대학교 공과대학 생물공학과) ;
  • 허원 (강원대학교 공과대학 생물공학과)
  • Received : 2010.01.19
  • Accepted : 2010.02.15
  • Published : 2010.07.25
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Abstract

Fibroin is a biopolymer available in large quantity from silk fiber and has a long history of use as a suture proving biocompatibility. In this report, fibroin microspheres has been fabricated for biomaterial applications. W/O emulsion of regenerated fibroin droplets in a continuous phase of decane with mixed surfactants was dried to facilitate fibroin gelation and the condensed fibroin microspheres were harvested. The ratio of mixed surfactants and their proportions to decane were determined to prepare a stable W/O emulsion. A spherical form of fibroin gels was obtained from the W/O emulsion agitated at 600 rpm. Scanning electron microscopy revealed that number average sizes of the fibroin microspheres were 21.6 and 8.5 ${\mu}m$ when dried under ambient conditions or under vacuum, respectively. Tomography of the spheres revealed that their internal structures are packed or hollowed. Hollow and hemispherical forms of microspheres were also prepared by using porogen.

피브로인은 실크섬유에서 대량으로 얻어지는 생물고분자이며 수술 봉합사로 장기간 사용되어 생체적합성이 확인되었다. 따라서, 본 연구에서는 생체재료로 사용할 수 있는 피브로인 미립구를 제조하였다. 데칸과 혼합유화제로 이루어진 연속상에 재생 피브로인 액적을 분산시킨 후 건조시켜 형성된 미립구를 회수하여 에탄올 등으로 세척 건조 후 수득하였다. 안정한 유중수형(W/O) 에멀션을 600 rpm으로 교반하는 조건하에서 구형의 피브로인 미립구를 얻 을 수 있었다. 주사전자현미경 사진을 통하여 상압 및 감압하에서 건조된 미립구의 수평균 직경은 각각 21.6 및 8.5 ${\mu}m$로 확인되었다. 토모그래피로 미립구는 내부구조가 채워져 있으나 크기에 따라 중공미립구임을 확인하였고 중탄 산나트륨을 도입하여 중공미립구도 제조하였다.

Keywords

References

  1. C. Z. Zhou, F. Confalonieri, M. Jacquet, R. Perasso, Z. G. Li, and J. Janin, Proteins, 44, 119 (2001). https://doi.org/10.1002/prot.1078
  2. G. H. Altman, F. Diaz, C. Jakuba, T. Calabro, R. L. Horan, J. Chen, H. Lu, J. Richmond, and D. L. Kaplan, Biomaterials, 24, 401 (2003). https://doi.org/10.1016/S0142-9612(02)00353-8
  3. X. Wang, E. Wenk, A. Matsumoto, L. Meinel, C. Li, and D. L. Kaplan, J. Control. Release, 117, 360 (2007). https://doi.org/10.1016/j.jconrel.2006.11.021
  4. E. Wenk, A. J. Wandrey, H. P. Merkle, and L. Meinel, J. Control. Release, 132, 26 (2008). https://doi.org/10.1016/j.jconrel.2008.08.005
  5. J. H. Yeo, K. G. Lee, Y. W. Lee, and S. Y. Kim, Eur. Polym. J., 39. 1195 (2003). https://doi.org/10.1016/S0014-3057(02)00359-2
  6. Z. Cao, X. Chen, J. Yao, L. Huang, and Z. Shao, Soft Matter, 3, 910 (2007). https://doi.org/10.1039/b703139d
  7. C. Z. Zhou, F. Confalonieri, N. Medina, Y. Zivanovic, C. Esnault, T. Yang, M. Jacquet, J. Janin, M. Duguet, R. Perasso, and Z. G. Li, Nucleic Acids Res., 28, 2413 (2000). https://doi.org/10.1093/nar/28.12.2413
  8. F. N. Braun and C. Viney, lnt. J. Biol. Macromol., 32, 59 (2003). https://doi.org/10.1016/S0141-8130(03)00038-2
  9. R. E. Marsh, R. B. Corey, and L. Pauling, Biochim. Biophys. Acta, 16, 1 (1955). https://doi.org/10.1016/0006-3002(55)90178-5
  10. H. Wang, Y. Zhang, H. Shao, and X. Hu, Int. J. Biol. Macromol., 36, 66 (2005). https://doi.org/10.1016/j.ijbiomac.2005.03.011
  11. T. Yucel. P. Cebe, and D. L. Kaplan, Biophys. J., 97, 2044 (2009) . https://doi.org/10.1016/j.bpj.2009.07.028
  12. R. Sugino, H. Masuda. J. Yao, T. Kameda, K. Enomoto, S. Amiya, A. Suzuki, and T. Asakura, Polymer Preprints(Japan), 49, 529 (2000).
  13. J. L. Salager, M. Perez-Sanchez, and Y. Garcia, Colloid. Polym. Sci., 274, 81 (1996) https://doi.org/10.1007/BF00658913
  14. W. Yu and M. Bousmina, J. Rheol., 47, 1011 (2003). https://doi.org/10.1122/1.1582853
  15. D. Megias-Alguacil, K. Feigl, M. Dressler, P. Fischer, and E. J. Windhab, J. Non-Newtonian Fluid Mech., 126, 153 (2005). https://doi.org/10.1016/j.jnnfm.2004.07.019
  16. X, Chen, Z. Shao, N, S. Marinkovic. L. M. Miller, P. Zhou. and M. R. Chance, Biophys. Chem., 89, 25 (2001). https://doi.org/10.1016/S0301-4622(00)00213-1
  17. G.-S. Choi, Y.-S. Kim, H.-S. Jung. S.-W. Jang. and N.-S. Kim, Polymer(Korea), 33, 463 (2009).
  18. H. Zhang, X. J. Ju, R. Xie, C. J. Cheng, P. W. Ren, and L. Y. Chu, J. Colloid Interface Sci., 336, 235 (2009). https://doi.org/10.1016/j.jcis.2009.03.070