Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Antiviral Activity of Seaweed Extracts against Feline Calicivirus

  • Kim, Kyoung-Lan (Department of Microbiology, Pukyong National University) ;
  • Lee, Dae-Sung (POSTECH Ocean Science and Technology Institute, Pohang University of Science and Technology (POSTECH)) ;
  • Park, Mi-Sun (Department of Microbiology, Pukyong National University) ;
  • Eom, Sung-Hwan (Department of Food Science & Technology, Pukyong National University) ;
  • Lim, Keun-Sik (Department of Food Science & Technology, Pukyong National University) ;
  • Kim, Jong-Soon (NGETECH Inc.) ;
  • Lee, Dong-Ho (Children's Dietary Life Safety Division, Korea Food and Drug Administration) ;
  • Kang, Chang-Keun (POSTECH Ocean Science and Technology Institute, Pohang University of Science and Technology (POSTECH)) ;
  • Kim, Young-Mog (Department of Food Science & Technology, Pukyong National University) ;
  • Lee, Myung-Suk (Department of Microbiology, Pukyong National University)
  • Received : 2010.04.05
  • Accepted : 2010.06.10
  • Published : 2010.06.30
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Abstract

Norovirus, which causes gastroenteritis in humans, is an important food-borne pathogen worldwide. In an effort to discover an antiviral substance against norovirus, extracts from several seaweeds were evaluated for antiviral activity against feline calicivirus (FCV), which was used as a surrogate. The methanolic extract of Undaria pinnatifida exhibited the most significant antiviral activity and virucidal efficacy against FCV. The concentrations of the extract that reduced viral replication by 50% ($EC_{50}$) and resulted in the death of 50% of the host cells ($CC_{50}$) were 0.05 mg/mL and 1.02 mg/mL, respectively. The selectivity index, calculated from the ratio of the $CC_{50}$ and $EC_{50}$ was 20.4. No FCV infection of host cells occurred following a 1-h incubation in the presence of 12.50 mg/mL U. pinnatifida extract, indicating that the virus was completely inactivated by the extract treatment. The results obtained in this study will contribute to the development of a natural antiviral substance that will prevent food-borne disease caused by norovirus.

Keywords

References

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