Maxillofacial Plastic and Reconstructive Surgery

  • 제32권4호
  • /
  • Pages.306-312
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  • 2010
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
대한악안면성형재건외과학회 (Korean Association of Maxillofacial Plastic and Reconstructive Surgeons)
권호 표지

가토골수에서 유래된 골모세포의 하악골 결손부 이식시 골형성에 미치는 효과

The Effect of Bone Marrow-Derived Osteoblasts on Mandibular Deffect in Rabbit

  • 박영주 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 남정훈 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 김보균 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 전민수 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 정재안 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 이정원 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 안장훈 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 강태인 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 박미희 (한림대학교 의과대학 강남성심병원 구강악안면외과학교실) ;
  • 임성철 (조선대학교 의과대학 병리학교실)
  • Park, Young-Ju (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Nam, Jeong-Hun (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Kim, Bo-Gyun (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Jeon, Min-Su (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Chung, Jae-An (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Lee, Jung-Won (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Ahn, Jang-Hoon (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Gang, Tae-In (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Park, Mi-Hee (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Lim, Sung-Chul (Department of Pathology, College of Medicine, Chosun University)
  • 투고 : 2010.05.31
  • 심사 : 2010.06.30
  • 발행 : 2010.07.30
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초록

Purpose: The purpose of this experiment was to evaluate the clinical effect of cultured autoglogous osteoblasts as a way to treat the defect of mandible in rabbits. Materials and Methods: Twelve rabbits were used to determine the rate of osteogenesis. The osteoblasts were obtained from the iliac crest of rabbits using aspiration. They were then cultured in Dulbecco's Modified Eagles's Medium (DMEM) with beta-glycerophosophatate, L-ascorbicacid, and dexamethasone to proliferate and differentiate osteoprogenitor cells. The expression of osteogenic markers were detected by reverse transcription-polymerase chain reaction (RT-PCR) and silver nitrate staining techniques. Five, 10-mm holes were placed in each rabbit mandible to simulate defective regions with the use of a low speed trephine bur. In the experimental group, the previously cited defects were grafted with both activated osteoblastic and autogenous bone. The control group, however, was only grafted with autogenous bone. Both groups were then analyzed at 2, 4, and 8-week intervals using bone histomorphometric analysis. Results: According to histomorphologic analysis, the rates of new bone formation at the 2, 4, and 8-week intervals were 36%, 51%, and 23% for the control group, respectively; 52%, 39%, and 28%, for the experimental group, respectively. The experimental group showed higher rates of new bone formation compared to the control group at both the 2-week and 8-week interval. Conclusion: Bone marrow-derived osteoblasts seems to be a promising bone graft material.

키워드

참고문헌

  1. Peetz M, Boyne PJ : Characterization of xenogeneic bone material. Quintessence, 1997, p.87.
  2. Association of korean maxillofacial plastic and reconstruction surgeons : Textbook of maxillofacial plastic and reconstructive surgery, 1st ed. Med-dent Co, 2004, p.100.
  3. Chen F, Ouyang H, Feng X et al : Anchoring Dental Implant in tissue engineered bone using composite scaffold a preliminary study in nude mouse model. J Oral Maxillofac Surg 63 : 586, 2005. https://doi.org/10.1016/j.joms.2005.01.007
  4. Paul C, Schelickewei W, Kuner EH et al : Bovines apatit- Wertigkeit beim Knochenersatz. Pesch HJ, Stoss. Osteologie aktuell. : 288, 1993.
  5. Yamada Y, Ueda M, Naiki T et al : Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma, Tissue engineered bone regeneration Tissue Engineering 10 : 955, 2004.
  6. Yamada Y, Ueda M, Naiki T et al : Tissue-engineered injectable bone regeneration for osseointegrated dental implants. Clin Oral Impl Res 15 : 589, 2004. https://doi.org/10.1111/j.1600-0501.2004.01038.x
  7. Erickson GR, Gimble JM, Franklin DM et al : Chondrogenic potential of adipose tissue-derived stromalcells in vitro and in vivo. Biochem Biophys Res Commun 290 : 763, 2002. https://doi.org/10.1006/bbrc.2001.6270
  8. Safford KM : Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem Biophys Res Commun 294 : 371, 2002 https://doi.org/10.1016/S0006-291X(02)00469-2
  9. Halvorsen YD : Extracellular matrix mineralization and osteoblast gene-expression by human adipose tissuederived stromal cells. Tissue Eng 7 : 729, 2001. https://doi.org/10.1089/107632701753337681
  10. Kadiyara S, Young RG, Thiede MA et al : Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro. Cell Transplantation 6 : 125, 1997. https://doi.org/10.1016/S0963-6897(96)00279-5
  11. Garg Ak : The future role of growth factors in bone grafting. Dent Implantol Update 10 : 395, 1999.
  12. Lind M : Growth factors : Possible new clinical tools. Acta Orthop Scand 67 : 407, 1996. https://doi.org/10.3109/17453679609002342
  13. Lanza RP, Chick W : Principles of tissue engineering. CA, San Diego, Academic Press, 2000, p.580.
  14. Parlette AC, Jose JM : Phenotypical and Functional Properties of Human Bone Marrow Mesenchymal Progenitor Cell. J cellular physiology 181 : 67, 1999. https://doi.org/10.1002/(SICI)1097-4652(199910)181:1<67::AID-JCP7>3.0.CO;2-C
  15. Scott PB, Barbara SF : Tissue Engineering of Bone Cell Based strategies. Clinical Orthopaedics & Related Research 367S : 68, 1999.
  16. Maniatopoulos C, Sodek J, Melcher AH et al : Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell tissue Res 254 : 317, 1988.
  17. Ohgushi H, Dohi Y, Yoshikawa T et al : Osteogenic differentiation of cultured marrow stromal stem cells on the surface of bioactive glass ceramics. J Biomed Mat Res 32 : 341, 1996. https://doi.org/10.1002/(SICI)1097-4636(199611)32:3<341::AID-JBM6>3.0.CO;2-S
  18. Ohgushi H, Yoshikawa T, Nakajima H et al : Dropped apatite-containing glass ceramic provokes osteogenic differentiation of marrow stromal stem cells. J Biomed Mater Res 44 : 381, 1999. https://doi.org/10.1002/(SICI)1097-4636(19990315)44:4<381::AID-JBM3>3.0.CO;2-E
  19. Ohya M, Yamada Y, Ozawa R et al : Sinus floor elevation applied tissue engineered bone. comparative study between mesenchymal cells, platelet-rich plasma(PRP) and autogenous bone with PRP complex in rabbits. Clin Oral Impl Res 16 : 622, 2005. https://doi.org/10.1111/j.1600-0501.2005.01136.x
  20. Bruder SP, Kurth AA, Shea M et al : Bone Regeneration by Implantation of purified, Culture-Expanded Human Mesenchymal Stem Cells. J Orthopedic Research 16 : 155, 1998. https://doi.org/10.1002/jor.1100160202
  21. Horisaka Y, Takagi T : Histological changes of implanted collagen material during bone induction. J Biomedical Materials Research 28 : 27, 1994. https://doi.org/10.1002/jbm.820280105