Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Development of Diagnosis Protocol for Micro-spike Biopsy Using Paraffin-based Tissue Collecting tool

파라핀 기반의 조직회수도구를 사용한 채취 조직의 진단 프로토콜 개발

  • Jeong, Hyo-Young (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University) ;
  • Koo, Kyo-In (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University) ;
  • Lee, Sang-Min (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University) ;
  • Park, Ho-Soo (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University) ;
  • Hong, Suk-Jun (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University) ;
  • Bang, Seoung-Min (Department of Internal Medicine, College of Medicine, Yonsei University) ;
  • Song, Si-Young (Department of Internal Medicine, College of Medicine, Yonsei University) ;
  • Cho, Dong-Il (ASRI / ISRC, School of Electrical Engineering and Computer Sciences, Seoul National University)
  • 정효영 (서울대학교 공과대학 전기컴퓨터공학부 자동화시스템연구소 / 반도체연구소) ;
  • 구교인 (서울대학교 공과대학 전기컴퓨터공학부 자동화시스템연구소 / 반도체연구소) ;
  • 이상민 (서울대학교 공과대학 전기컴퓨터공학부 자동화시스템연구소 / 반도체연구소) ;
  • 박호수 (서울대학교 공과대학 전기컴퓨터공학부 자동화시스템연구소 / 반도체연구소) ;
  • 홍석준 (서울대학교 공과대학 전기컴퓨터공학부 자동화시스템연구소 / 반도체연구소) ;
  • 방승민 (연세대학교 의과대학 소화기내과) ;
  • 송시영 (연세대학교 의과대학 소화기내과) ;
  • 조동일 (서울대학교 공과대학 전기컴퓨터공학부 자동화시스템연구소 / 반도체연구소)
  • Received : 2009.11.18
  • Accepted : 2010.04.04
  • Published : 2010.06.30
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Abstract

We have developed and reported several micro-spikes for minimally invasive biopsy. This paper presents a diagnosis protocol for micro-spike biopsy using paraffin-based tissue collecting tool. Using the proposed tissue collecting tool, which has a negative micro-spike structure in a porous chamber, the biopsied tissue in a micro-spike is effectively detached. The proposed diagnosis protocol prevents the loss of tissues in a paraffin embedding and sectioning process. Hence, it is compatible with conventional histopathology without additional reagents and processes. The gastro-intestinal tissue of a pig is biopsied in an in vivo environment, and then it is detached from a micro-spike using the paraffin-based tissue collecting tool. A histopathological photomicrograph of the detached tissue is acquired with the proposed diagnosis protocol. The acquired image offers clinical quality. This result shows that the paraffin-based tissue collecting tool is applicable to the medical practice.

Keywords

References

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