Parasites, Hosts and Diseases

  • 제48권2호
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  • Pages.127-132
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  • 2010
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Carboxylic Acids as Biomarkers of Biomphalaria alexandrina Snails Infected with Schistosoma mansoni

  • Abou Elseoud, Salwa M. F. (Parasitology Department, Faculty of Medicine, Ain Shams University) ;
  • Fattah, Nashwa S. Abdel (Parasitology Department, Faculty of Medicine, Ain Shams University) ;
  • El Din, Hayam M. Ezz (Parasitology Department, Faculty of Medicine, Ain Shams University) ;
  • Abdel Al, Hala (Clinical Pathology Department, Faculty of Medicine, Ain Shams University) ;
  • Mossalem, Hanan (Medical Malacology Department, Theodor Bilharz Research Institute) ;
  • Elleboudy, Noha (Parasitology Department, Faculty of Medicine, Ain Shams University)
  • 투고 : 2010.03.19
  • 심사 : 2010.05.07
  • 발행 : 2010.06.30
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초록

Biomphalaria alexandrina snails play an indispensable role in transmission of schistosomiasis. Infection rates in field populations of snails are routinely determined by cercarial shedding neglecting prepatent snail infections, because of lack of a suitable method for diagnosis. The present study aimed at separation and quantification of oxalic, malic, acetic, pyruvic, and fumaric acids using ion-suppression reversed-phase high performance liquid chromatography (HPLC) to test the potentiality of these acids to be used as diagnostic and therapeutic biomarkers. The assay was done in both hemolymph and digestive gland-gonad complex (DGG) samples in a total of 300 B. alexandrina snails. All of the studied acids in both the hemolymph and tissue samples except for the fumaric acid in hemolymph appeared to be good diagnostic biomarkers as they provide not only a good discrimination between the infected snails from the control but also between the studied stages of infection from each other. The most sensitive discriminating acid was malic acid in hemolymph samples as it showed the highest F-ratio. Using the Z-score, malic acid was found to be a good potential therapeutic biomarker in the prepatency stage, oxalic acid and acetic acid in the stage of patency, and malic acid and acetic acid at 2 weeks after patency. Quantification of carboxylic acids, using HPLC strategy, was fast, easy, and accurate in prediction of infected and uninfected snails and possibly to detect the stage of infection. It seems also useful for detection of the most suitable acids to be used as drug targets.

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피인용 문헌

  1. Potential Correlation between Carboxylic Acid Metabolites in Biomphalaria alexandrina Snails after Exposure to Schistosoma mansoni Infection vol.50, pp.2, 2010, https://doi.org/10.3347/kjp.2012.50.2.119
  2. Advances in the Diagnosis of Human Schistosomiasis vol.28, pp.4, 2010, https://doi.org/10.1128/cmr.00137-14
  3. Unveiling the oxidative metabolism of Achatina fulica (Mollusca: Gastropoda) experimentally infected to Angiostrongylus cantonensis (Nematoda: Metastrongylidae) vol.117, pp.6, 2010, https://doi.org/10.1007/s00436-018-5859-x
  4. UHPLC-MS-Based Metabolomics Analysis Reveals the Process of Schistosomiasis in Mice vol.11, pp.None, 2020, https://doi.org/10.3389/fmicb.2020.01517