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Biological and Structural Mechanisms of Disease Development and Resistance in Chili Pepper Infected with the Root-knot Nematode

  • Moon, Hyo-Sun (Department of Agricultural Biotechnology, Seoul National University) ;
  • Khan, Zakaullah (Germplasm Evaluation Division, National Bureau of Plant Genetic Resources) ;
  • Kim, Sang-Gyu (Department of Agricultural Biotechnology, Seoul National University) ;
  • Son, Seon-Hye (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Young-Ho (Department of Agricultural Biotechnology, Seoul National University)
  • Received : 2009.11.24
  • Accepted : 2010.01.06
  • Published : 2010.06.30

Abstract

Biological and structural mechanisms of the nematode disease development in chili pepper caused by the root-knot nematode, Meloidogyne incognita, were investigated. Out of 39 chili pepper cultivars/lines tested, six were found resistant, while 33 were susceptible to M. incognita, of which a susceptible cultivar Chilseongcho and three resistant cultivar/lines CM334, 02G132 and 03G53 with different resistance degrees were selected for microscopic studies on the disease development. Gall formation was greatly reduced in the resistant cultivars/lines. Nematode penetration occurred both in the susceptible and resistant chili pepper roots; however, the penetration rates were significantly lowered in the three resistant peppers compared to the susceptible pepper cv. Chilseongcho. In the susceptible pepper, giant cells were extensively formed with no discernible necrosis around the nematode feeding sites. In the highly resistant pepper cultivar CM334, no giant cell was formed, but extensive necrosis formation was observed around the penetrating nematodes. In the other two resistant pepper lines (02G132 and 03G53), both giant cells and prominent necroses were formed, and the necrotic responses appeared to inhibit the further development of giant cells or accelerate their early degeneration. Although the nematode penetration was retarded significantly in the resistant cultivar/lines, all of the above results suggest that the disease resistance of pepper may be related to post-infectional defense mechanisms (nematode growth and development) more than pre-infectional ones (penetration and establishment). Variations in structural modifications in the resistant cultivar/lines may reflect their genetic differences related to the nematode resistance.

Keywords

References

  1. Agrios, G. N. 2005. Plant Pathology, Fifth edition. Academic Press, San Diego, CA. 922 pp.
  2. Anwar, S. A. and McKenry, M. V. 2000. Penetration, development and reproduction of Meloidogyne arenaria on two new resistant Vitis spp. Nematropica 30:9-17.
  3. Barker, K. 1998. Introduction and synopsis of advancements in nematology. In: Plant Nematode Interactions, ed. by K. R. Barker, G. A. Pederson and G. L. Windham, pp. 1-20. American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, Madison, WI.
  4. Canto-Saenz, M. and Brodie, B. B. 1987. Comparison of compatible and incompatible response of potato to Meloidogyne incognita. J. Nematol. 19:218-221.
  5. Dropkin, V. H. and Nelson, P. E. 1960. The histopathology of root-knot nematode infections in soybeans. Phytopathology 50:442-447.
  6. Hartman, K. M. and Sasser, J. N. 1985. Identification of Meloidogyne species on the basis of differential host test and perennial-pattern morphology. In: An Advanced Treatise on Meloidogyne, Volume 11: Methodology, ed. by K. R. Barker, C. C. Carter and J. N. Sasser, pp. 69-78. North Carolina State University Graphics, Raleigh, NC.
  7. Hayne, R. L. and Jones, C. M. 1976. Effects of the Bi locus in cucumber on reproduction, attraction, and response of the plant to the southern root knot nematode. J. Am. Soc. Hortic. Sci. 101:422-424.
  8. Jones, M. G. K. 1981. Host cell responses to endoparasitic attack: structure and function of giant cells and syncytia. Ann. Appl. Biol. 97:353-72. https://doi.org/10.1111/j.1744-7348.1981.tb05122.x
  9. Kaplan, D. T. and Keen, N. T. 1980. Mechanisms conferring plant incompatibility to nematodes. Rev. Nématol. 3:123-134.
  10. Khan, Z., Kim, S. G., Jeon, Y. H., Khan, H. U., Son, S. H. and Kim, Y. H. 2008. A growth promoting rhizobacterium, Paenibacillus polymyxa strain GBR-1, suppresses root-knot nematode. Biores. Technol. 3016-3023.
  11. Kim, S. G. and Kim, Y. H. 2009. Histological and cytological changes associated with susceptible and resistant responses of chili pepper root and stem to Phytophthora capsici infection. Plant Pathol. J. 25:113-120. https://doi.org/10.5423/PPJ.2009.25.2.113
  12. Kim, Y. H., Riggs, R. D. and Kim, K. S. 1986. A mechanism of density dependent population change in Heterodera glycines. Korean J. Plant Pathol. 2:199-206.
  13. Oka, Y., Koltai, H., Bar-Eyal, M., Mor, M., Sharon, E., Chet, I. and Spiegel, Y. 2000. New strategies for the control of plantparasitic nematodes. Pest Management Sci. 56:983-988. https://doi.org/10.1002/1526-4998(200011)56:11<983::AID-PS233>3.0.CO;2-X
  14. Paulson, R. E. and Webster, J. M. 1972. Ultrastructure of the hypersensitive reaction in roots of tomato, Lycopersicon esculentum L., to infection by the root-knot nematode, Meloidogyne incognita. Physiol. Plant Pathol. 2:227-234. https://doi.org/10.1016/0048-4059(72)90005-7
  15. Pegard, A. and Brizzard, G. 2005. Histological characterization of resistance to different root-knot nematode species related to phenolics accumulation in Capsicum annuum. Nematology 95:158-165.
  16. Pontier, D., Gan, S. S., Amasino, R. M., Roby, D. and Larn, E. 1999. Markers for hypersensitive response and senescence show distinct patterns of expression. Plant Mol. Biol. 39:1243-1255. https://doi.org/10.1023/A:1006133311402
  17. Sasser, J. N. 1977. Worldwide dissemination and importance of the root knot nematode, Meloidogyne spp. J. Nematol. 22:585-589.
  18. Sasser, J. N., Carter, C. C. and Hartman, K. M. 1984. Standardization of host suitability studies and reporting of resistance to root-knot nematodes. Crop Nematode Res. Control Proj., NCSU/USAID. Dept. of Plant Pathol., NCSU, Box 7616, Raleigh, North Carolina 27695, USA. 7 pp.
  19. Shepherd, R. L. and Huck, M. G. 1989. Progression of root-knot nematode symptoms and infection on resistant and susceptible cottons. J. Nematol. 21:235-241.
  20. Southey, J. F. 1986. Laboratory Methods for Work with Plant and Soil Nematodes. Ministry of Agriculture Fisheries and Food, HMSO, London, UK.
  21. Spurr, A. R. 1969. A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastr. Res. 26:31-43. https://doi.org/10.1016/S0022-5320(69)90033-1
  22. Tanda, A. S., Atwal, A. S. and Bajaj, Y. P. S. 1989. In vitro inhibition of root-knot nematode Meloidogyne incognita by sesame root-exudate and its amino acids. Nematologica 35:115-124. https://doi.org/10.1163/002825989X00124
  23. Taylor, A. L. and Sasser, J. N. 1978. Biology, Identification and Control of Root-knot Nematodes, Meloidogyne Species. International Meloidogyne Project, Department of Plant Pathology, North Carolina State University and the U.S. Agency for International Development, Raleigh, North Carolina, U.S.A. 111 pp.

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