Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Biocompatibility of experimental mixture of mineral trioxide aggregate and glass ionomer cement

Glass ionomer cement와 혼합한 mineral trioxide aggregate의 생체친화성

  • Oh, Min-Jae (Dept. of Conservative Dentistry, Chonnam National University School of Dentistry) ;
  • Jeong, Yu-Na (Dept. of Conservative Dentistry, Chonnam National University School of Dentistry) ;
  • Bae, In-Ho (Dept. of Pharmacology and Dental Therapeutics, Chonnam National University School of Dentistry) ;
  • Yang, So-Young (Dept. of Oral Anatomy, Chonnam National University School of Dentistry) ;
  • Park, Bum-Jun (Jeollanam-do Institute of Health and Environment) ;
  • Koh, Jeong-Tae (Dept. of Pharmacology and Dental Therapeutics, Chonnam National University School of Dentistry) ;
  • Hwang, Yun-Chan (Dept. of Conservative Dentistry, Chonnam National University School of Dentistry) ;
  • Hwang, In-Nam (Dept. of Conservative Dentistry, Chonnam National University School of Dentistry) ;
  • Oh, Won-Mann (Dept. of Conservative Dentistry, Chonnam National University School of Dentistry)
  • 오민제 (전남대학교치의학전문대학원, 보존학교실) ;
  • 정유나 (전남대학교치의학전문대학원, 보존학교실) ;
  • 배인호 (전남대학교치의학전문대학원, 약리학교실) ;
  • 양소영 (전남대학교치의학전문대학원, 해부학교실) ;
  • 박범전 (전라남도보건환경연구원) ;
  • 고정태 (전남대학교치의학전문대학원, 약리학교실) ;
  • 황윤찬 (전남대학교치의학전문대학원, 보존학교실) ;
  • 황인남 (전남대학교치의학전문대학원, 보존학교실) ;
  • 오원만 (전남대학교치의학전문대학원, 보존학교실)
  • Received : 2010.07.02
  • Accepted : 2010.08.17
  • Published : 2010.09.30
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Abstract

Objectives: The purpose of the present in vitro study was to evaluate the biocompatibility of mineral trioxide aggregate (MTA) mixed with glass ionomer cement (GIC), and to compare it with that of MTA, GIC, IRM and SuperEBA. Materials and Methods: Experimental groups were divided into 3 groups such as 1 : 1, 2 : 1, and 1 : 2 groups depending on the mixing ratios of MTA powder and GIC powder. Instead of distilled water, GIC liquid was mixed with the powder. This study was carried out using MG-63 cells derived from human osteosarcoma. They were incubated for 1 day on the surfaces of disc samples and examined by scanning electron microscopy. To evaluate the cytotoxicity of test materials quantitatively, XTT assay was used. The cells were exposed to the extracts and incubated. Cell viability was recorded by measuring the optical density of each test well in reference to controls. Results: The SEM revealed that elongated, dense, and almost confluent cells were observed in the cultures of MTA mixed with GIC, MTA and GIC. On the contrary, cells on the surface of IRM or SuperEBA were round in shape. In XTT assay, cell viability of MTA mixed with GIC group was similar to that of MTA or GIC at all time points. IRM and SuperEBA showed significantly lower cell viability than other groups at all time points (p < 0.05). Conclusions: In this research MTA mixed with GIC showed similar cellular responses as MTA and GIC. It suggests that MTA mixed with GIC has good biocompatibility like MTA and GIC.

연구목적: 본 연구의 목적은 glass ionomer cement (GIC)와 혼합된 mineral trioxide aggregate (MTA)의 생체친화성을 평가하고 이것을 MTA, GIC, IRM, SuperEBA와 비교해보는 것이다. 연구 재료 및 방법: 재료의 세포독성을 평가하기 위해 MG-63세포를 이용해 주사전자 현미경 관찰과 XTT assay를 실시하였다. 결과: 주사전자 현미경 관찰에서는 GIC와 혼합한 MTA, MTA, GIC의 표면에서 세포질 돌기를 가진 많은 세포들이 밀집되고 융합된 형태로 관찰되었다. 반면 IRM과 SuperEBA에서는 세포들의 수가 적고 둥근 양상을 보여주었다. XTT assay에서는 GIC와 혼합한 MTA에서의 세포 활성도는 모든 시점에서 MTA 또는 GIC와 유사하였다. 반면 IRM과 SuperEBA에서는 모든 시점에서 세포활성도가 다른 그룹에 비해 유의하게 더 낮았다. 결론: 본 연구에서 GIC와 혼합된 MTA는 MTA, GIC와 유사한 세포 반응을 나타냈다. 이것은 GIC와 혼합된 MTA가 MTA, GIC와 마찬가지로 좋은 생체친화성을 가진 재료라는 것을 시사한다.

Keywords

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