Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
권호 표지
DOI QR코드

DOI QR Code

Real-time measurement of dentinal fluid flow during desensitizing agent application

상아질 지각과민 처치제의 적용 중에 일어나는 상아세관액 유동의 실시간 측정

  • Kim, Sun-Young (Department of Conservative Dentistry, KyungHee University School of Dentistry) ;
  • Kim, Eun-Joo (Department of Dental Hygiene, Dong-Nam Health University) ;
  • Lee, In-Bog (Department of Conservative Dentistry, Seoul National University School of Dentistry)
  • 김선영 (경희대학교 치의학대학원 치과보존학교실) ;
  • 김은주 (동남보건대학 치위생과) ;
  • 이인복 (서울대학교 치의학대학원 치과보존학교실)
  • Received : 2010.04.23
  • Accepted : 2010.04.27
  • Published : 2010.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The aim of this study was to examine changes in the dentinal fluid flow (DFF) during desensitizing agent application and to compare permeability after application among the agents. Materials and Methods: A Class 5 cavity was prepared to exposure cervical dentin on an extracted human premolar which was connected to a sub-nanoliter fluid flow measuring device (NFMD) under 20 cm water pressure. DFF was measured from before application of desensitizing agent (Seal&Protect, SP; SuperSeal, SS; BisBlock, BB; Gluma desensitizer, GL; Bi-Fluoride 12, BF) through application procedure to 5 min after application. Results: DFF rate after each desensitizing agent application was significantly reduced when compared to initial DFF rate before application (p < 0.05). SP showed a greater reduction in DFF rate than GL and BF did (p < 0.05). SS and BB showed a greater reduction in DFF rate than BF did (p < 0.05). Conclusions: Characteristic DFF aspect of each desensitizing agent was shown in NFMD during the application procedure.

연구목적: 본 연구의 목적은 미세 유체 흐름 측정장치를 이용해서 각 상아질 지각과민 처치제의 적용 과정 중 발생하는 상아 세관액 흐름의 유동을 관찰하고, 상아질 지각과민 처치제 간 적용 후 투과도 감소의 차이를 비교하고자 하는 것이다. 연구 재료 및 방법: CEJ 5 mm 하방에서 절단한 소구치를 미세 흐름 측정장치에 연결하여 20 cm $H_2O$의 정수압을 가하였다. 치경부에 상하 3 mm, 근원심 4 mm, 깊이 2 mm의 V자 형태의 5급 와동을 형성하고 smear layer를 제거하기 위해 15초 동안 산 부식한 후 상아질 지각과민 처치제를 적용하였다. 사용된 상아질 지각과민 처치제는 Seal&Protect (SP), SuperSeal (SS), BisBlock (BB), Gluma desensitizer (GL), Bifluoride 12 (BF) 이다. 상아세관액의 흐름은 지각과민 처치제 도포 전부터 도포 후 5분까지 실시간으로 측정되었다. 결과: 실험에 사용한 모든 상아질 지각과민 처치제는 적용 후의 상아세관액 흐름율이 적용 전에 비해 유의하게 감소하였다(p < 0.05). SP는 GL과 BF에 비해 유의하게 투과도 감소가 나타났다 (p < 0.05). SS와 BB는 BF에 비해 유의하게 투과도 감소를 보였다 (p < 0.05). 결론: 지각과민 처치제의 도포 과정 동안 각 처치제의 특징적인 상아세관액의 유동을 미세 유체 흐름 측정장치를 통해 관찰 할 수 있었다.

Keywords

References

  1. Brannstrom M, Linden LA, Astrom A. The hydrodynamics of the dental tubule and of pulp fluid. A discussion of its significance in relation to dentinal sensitivity. Caries Res 1967;1:310-317. https://doi.org/10.1159/000259530
  2. Pashley DH. Dentin permeability, dentin sensitivity, and treatment through tubule occlusion. J Endod 1986;12:465-474. https://doi.org/10.1016/S0099-2399(86)80201-1
  3. Absi EG, Addy M, Adams D. Dentine hypersensitivity. A study of the patency of dentinal tubules in sensitive and non-sensitive cervical dentine. J Clin Periodontol 1987;14:280-284. https://doi.org/10.1111/j.1600-051X.1987.tb01533.x
  4. Pashley DH. Potential treatment modalities for dentine hypersensitivity: in-office products. In: Tooth wear and sensitivity: clinical advances in restorative dentistry, Addy M, Embrey G, Edgar WM, Orchardson R eds, Martis Dunitz, London, 2000.351-365.
  5. Vieira AH, Santiago SL. Management of dentinal hypersensitivity. Gen Dent 2009;57:120-126.
  6. Lee IB, Kim MH, Kim SY, Chang J, Cho BH, Son HH, Back SH, Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid. J Kor Acad Cons Dent 2008;33:141-147. https://doi.org/10.5395/JKACD.2008.33.2.141
  7. Kim SY, Cho BH, Baek SH, Lim BS, Lee IB, Real-time measurement of dentinal tubular fluid flow during and after amalgam and composite restorations. J Kor Acad Cons Dent 2009;34:467-476. https://doi.org/10.5395/JKACD.2009.34.6.467
  8. Ciucchi B, Bouillaguet S, Holz J, Pashley D. Dentinal fluid dynamics in human teeth, in vivo. J Endod 1995;21:191-194. https://doi.org/10.1016/S0099-2399(06)80564-9
  9. Pashley DH, Stewart FP, Galloway SE. Effects of airdrying in vitro on human dentine permeability. Arch Oral Biol 1984;29:379-383. https://doi.org/10.1016/0003-9969(84)90164-X
  10. Itthagarun A, Tay FR, Pashley DH, Wefel JS, Garcla-Godoy F, Wei SH. Single-step, self-etch adhesives behave as permeable membranes after polymerization. Part III. Evidence from fluid conductance and artificial caries inhibition. Am J Dent 2004;17:394-400.
  11. Camps J, About I, Van Meerbeek B, Franquin JC. Efficiency and cytotoxicity of resin-based desensitizing agents. Am J Dent 2002;15:300-304.
  12. Pamir T, Dalgar H, Onal B. Clinical evaluation of three desensitizing agents in relieving dentin hypersensitivity. Oper Dent 2007;32:544-548. https://doi.org/10.2341/07-5
  13. Pereira JC, Segala AD, Gillam DG. Effect of desensitizing agents on the hydraulic conductance of human dentin subjected to different surface pre-treatments--an in vitro study. Dent Mater 2005;21:129-138. https://doi.org/10.1016/j.dental.2004.02.007
  14. Pashley DH, Galloway SE. The effects of oxalate treatment on the smear layer of ground surfaces of human dentine. Arch Oral Biol 1985;30:731-737. https://doi.org/10.1016/0003-9969(85)90185-2
  15. Greenhill JD, Pashley DH. The effects of desensitizing agents on the hydraulic conductance of human dentin in vitro. J Dent Res 1981;60:686-698. https://doi.org/10.1177/00220345810600030401
  16. Paes Leme AF, dos Santos JC, Giannini M, Wada RS. Occlusion of dentin tubules by desensitizing agents. Am J Dent 2004;17:368-372.
  17. Thrash WJ, Jones DL, Dodds WJ. Effect of a fluoride solution on dentinal hypersensitivity. Am J Dent 1992;5:299-302.
  18. Yates RJ, Newcombe RG, Addy M. Dentine hypersensitivity: a randomised, double-blind placebo-controlled study of the efficacy of a fluoride-sensitive teeth mouthrinse. J Clin Periodontol 2004;31:885-889. https://doi.org/10.1111/j.1600-051X.2004.00581.x
  19. Qin C, Xu J, Zhang Y. Spectroscopic investigation of the function of aqueous 2-hydroxyethylmethacrylate/ glutaraldehyde solution as a dentin desensitizer. Eur J Oral Sci 2006;114:354-359. https://doi.org/10.1111/j.1600-0722.2006.00382.x
  20. Munksgaard EC. Amine-induced polymerization of aqueous HEMA/aldehyde during action as a dentin bonding agent. J Dent Res 1990;69:1236-1239. https://doi.org/10.1177/00220345900690060201
  21. Kakaboura A, Rahiotis C, Thomaidis S, Doukoudakis S. Clinical effectiveness of two agents on the treatment of tooth cervical hypersensitivity. Am J Dent 2005;18: 291-295.
  22. Ozen T, Orhan K, Avsever H, Tunca YM, Ulker AE, Akyol M. Dentin hypersensitivity: a randomized clinical comparison of three different agents in a short-term treatment period. Oper Dent 2009;34:392-398. https://doi.org/10.2341/08-118
  23. Davidson DF, Suzuki M. The Gluma bonding system: a clinical evaluation of its various components for the treatment of hypersensitive root dentin. J Can Dent Assoc 1997;63:38-41.
  24. Ishihata H, Kanehira M, Nagai T, Finger WJ, Shimauchi H, Komatsu M. Effect of desensitizing agents on dentin permeability. Am J Dent 2009;22: 143-146.