Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Profiles of Glucocorticoid Receptor mRNA Expression and Physiological Changes in Response to Osmotic and Thermal Stress Conditions in Black Porgy (Acanthopagrus schlegeli)

염분과 수온 스트레스에 따른 감성돔의 glucocorticoid receptor mRNA 발현 특징과 생리적 변화에 관한 연구

  • An, Kwang-Wook (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Shin, Hyun-Suk (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Min, Byung-Hwa (Genetics and Breeding Research Center, National Fisheries Research & Development Institute) ;
  • Kil, Gyung-Suk (Division of Electrical & Electronic Engineering, Korea Maritime University) ;
  • Choi, Cheol-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 안광운 (한국해양대학교 해양환경.생명과학부) ;
  • 신현숙 (한국해양대학교 해양환경.생명과학부) ;
  • 민병화 (국립수산과학원 육종연구센터) ;
  • 길경석 (한국해양대학교 전기전자공학부) ;
  • 최철영 (한국해양대학교 해양환경.생명과학부)
  • Received : 2010.02.22
  • Accepted : 2010.03.23
  • Published : 2010.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The present study investigated the expression of glucocorticoid receptor (GR) mRNA as a stress response during salinity changes (35, 10, and 0 psu) and water temperature changes (from $20^{\circ}C$ to $30^{\circ}C$, $1^{\circ}C$/day) in black porgy. We cloned the full-length GR cDNA from the kidney and examined its expression in the gill, kidney, and intestine by quantitative real-time PCR (QPCR) during salinity and water temperature changes. During salinity changes, the levels of GR mRNA in the gill, kidney, and intestine were highest at 0 psu, and the levels of plasma cortisol and glucose were elevated, but triiodothyronine ($T_3$) decreased. Also, during water temperature changes, the levels of GR mRNA in the gill, kidney, and intestine increased at $30^{\circ}C$. Plasma parameters also increased with an increase in water temperature. Therefore, this upregulation of GR mRNA was a good indicator of stress, such as those resulting from changes in salinity and water temperature.

본 연구에서는 감성돔의 염분과 수온 변화에 따른 스트레스 반응을 알아보기 위하여 glucocorticoid receptor (GR) mRNA 발현을 조사하였다. 감성돔 신장으로부터 전장의 GR cDNA를 클로닝하였고, 염분과 수온이 변화하는 동안 아가미, 신장 및 장에서 GR mRNA 발현 변화를 quantitative real-time PCR (QPCR)을 이용하여 조사하였다. 염분 변화시, 아가미, 신장 및 장에서 GR mRNA 발현은 0 psu에서 가장 높게 나타났으며, 혈장 cortisol과 glucose 농도도 증가한 반면, triiodothyronine ($T_3$) 농도는 감소하였다. 수온 변화시, 아가미, 신장 및 장에서 GR mRNA 발현은 $30^{\circ}C$에서 가장 높게 관찰되었다. 혈장 cortisol, glucose 및 $T_3$ 농도 또한 고수온 ($30^{\circ}C$)에서 증가하였다. GR mRNA 발현의 증가는 염분과 수온 변화와 같은 환경 요인에 대한 좋은 스트레스 지표로 여겨진다.

Keywords

References

  1. Acerete, L., J.C. Balasch, B. Castellana, B. Redruello, N. Roher, A.V. Canario, J.V. Planas, S. MacKenzie and L. Tort. 2007. Cloning of the glucocorticoid receptor (GR) in gilthead seabream(Sparus aurata). Differential expression of GR and immune genes in gilthead seabream after an immune challenge. Comp. Biochem. Physiol. B, 148: 32-43. https://doi.org/10.1016/j.cbpb.2007.04.015
  2. Ackerman, P.A., R.B. Forsyth, C.F. Mazur and G.K Iwama. 2000. Stress hormones and the cellular stress response in salmonids. Fish Physiol. Biochem., 23: 327-336. https://doi.org/10.1023/A:1011107610971
  3. Aluru, N., E.H. Jorgensen, A.G. Maule and M.M. Vijayan. 2004. PCB disruption of the hypothalmus-pituitary-interrenal axis involves brain glucocorticoid receptor downregulation in anadromous Arctic charr. Am. J. Physiol. Regul. Integr. Comp. Physiol., 287: R787-R793. https://doi.org/10.1152/ajpregu.00091.2004
  4. Arjona, F.J., L. Vargas-Chacoff, I. Ruiz-Jarabo, M.P. Martin del Rio and J.M. Mancera. 2007. Osmoregulatory response of Senegalse sole (Solea senegalensis) to changes in environmental salinity. Comp. Biochem. Physiol. A, 148: 413-421. https://doi.org/10.1016/j.cbpa.2007.05.026
  5. Barton, B.A. 2002. Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integr. Comp. Biol., 42: 517-525. https://doi.org/10.1093/icb/42.3.517
  6. Barton, B.A. and C.B. Schreck. 1987. Influence of acclimation temperature on interrenal and carbohydrate stress responses in juvenile chinook salmon(Oncorhynchus tshawytsc). Aquaculture, 62: 299-310. https://doi.org/10.1016/0044-8486(87)90172-4
  7. Barton, B.A. and G.K Iwama. 1991. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu. Rev. Fish. Dis., 1: 3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  8. Beato, M., S.M. Chavez and M. Truss. 1996. Transcriptional regulation by steroid hormones. Steroids, 61: 240-251. https://doi.org/10.1016/0039-128X(96)00030-X
  9. Beckmann, R.P., L.E. Mizzen and W.J. Welch. 1990. Interaction of HSP70 with newly synthesized proteins: implications for protein folding and assembly. Science, 248: 850-854. https://doi.org/10.1126/science.2188360
  10. Bleau, H., C. Daniel, G. Chevalier, H. van Tra and A. Hontela. 1996. Effects of acute exposure to mercury chloride and methylmercury on plasma cortisol, $T_3$, $T_4$, glucose and liver glycogen in rainbow trout (Oncorhynchus mykiss). Aquat. Toxicol., 34: 221-235. https://doi.org/10.1016/0166-445X(95)00040-B
  11. Carmichael, G.J., J.R Tomasso, B.A. Simco and K.B. Davis. 1984. Characterization and alleviation of stress associated with hauling largemouth bass. Trans. Am. Fish. Soc., 113: 778-785. https://doi.org/10.1577/1548-8659(1984)113<778:CAAOSA>2.0.CO;2
  12. Davis, K.B. 2004. Temperature affects physiological stress responses to acute confinement in sunshine bass (Morone chrysops ${\times}$ Morone saxatilis). Comp. Biochem. Physiol. A, 139: 433-440. https://doi.org/10.1016/j.cbpb.2004.09.012
  13. Filby, A.L. and C.R. Tyler. 2007. Cloning and characterization of cDNAs for hormones and/or receptors of growth hormone, insulin-like growth factor-I, thyroid hormone, and corticosteroid and the gender-, tissue-, and developmental-specific expression of their mRNA transcripts in fathead minnow(Pimephales promelas). Gen. Comp. Endocrinol., 150: 151-163. https://doi.org/10.1016/j.ygcen.2006.07.014
  14. Greenwood, A.K, P.C. Butler, R.B. White, U. DeMarco, D. Pearce and R.D. Fernald. 2003. Multiple corticosteroid receptors in a teleost Fish: Distinct sequences, expression patterns, and transcriptional activities. Endocrinology, 144: 4226-4236. https://doi.org/10.1210/en.2003-0566
  15. Ishioka, H. 1980. Stress reaction in the marine fish. Stress reactions induced by temperature changes. Bull. Jpn. Soc. Sci. Fish., 46: 523-531. https://doi.org/10.2331/suisan.46.523
  16. Kiilerich, P., K Kristiansen and S.S. Madsen. 2007. Hormone receptors in gills of smolting Atlantic salmon, Salmo salar: Expression of growth hormone, prolactin, mineralocorticoid and glucocorticoid receptors and 11b hydroxysteroid dehydrogenase type 2. Gen. Comp. Endocrinol., 152: 295-303. https://doi.org/10.1016/j.ygcen.2006.12.018
  17. Lankford, S.E., T.E. Adams and J.J. Cech. 2003. Time of day and water temperature modify the physiological stress response in green sturgeon, Acipenser medirostris. Comp. Biochem. Physiol. A, 135: 291-302. https://doi.org/10.1016/S1095-6433(03)00075-8
  18. McCormick, S.D. 2001. Endocrine control of osmoregulation in fish. Am. Zool., 282: 290-300.
  19. Min, B.H., B.K. Kim, J.W. Hur, I.C. Bang, S.K. Byun, C.Y. Choi and Y.J. Chang. 2003. Physiological responses during freshwater acclimation of seawater-cultured black porgy (Acanthopagrus schlegeli). Korean J. Ichthyol., 15: 224-231. (in Korean)
  20. Min, B.H., G.A. Noh, M.H. Jeong, D.Y. Kang, C.Y. Choi, I.C. Bang and Y.J. Chang. 2006. Effects of oral administration of thyroid hormone on physiological activity and growth of black porgy reared in freshwater or seawater. J. Aquacult., 19: 149-156. (in Korean)
  21. Park, C.B, A. Takemura, N. Alum, Y.J. Park, B.H. Kim, C.H. Lee, Y.D. Lee, T.W. Moon and M.M. Vijayan. 2007. Tissue-specific suppression of estrogen, androgen and glucocorticoid receptor gene expression in feral vitellogenic male Mozambique tilapia. Chemosphere, 69: 32-40. https://doi.org/10.1016/j.chemosphere.2007.04.072
  22. Sathiyaa, R. and M.M. Vijayan. 2003. Autoregulation of glucocorticoid receptor by cortisol in rainbow trout hepatocytes. Am. J. Physiol. Cell. Physiol., 284: C1508-C1515. https://doi.org/10.1152/ajpcell.00448.2002
  23. Singer, T.D., S. Raptis, R Sathiyaa, J.W. Nichols, R.C, Playle and M.M. Vijayan. 2007. Tissue-specific modulation of glucocorticoid receptor expression in response to salinity acclimation in rainbow trout. Compo Biochem. Physiol. B, 146: 271-278. https://doi.org/10.1016/j.cbpb.2006.11.010
  24. Specker, J.L., T.A. Whitesel, S.J. Parker and R.L. Saunders. 1989. Thyroidal response of Atlantic salmon to seawater challenge: predictor of growth in seawater. Aquaculture, 82: 307-318. https://doi.org/10.1016/0044-8486(89)90417-1
  25. Takahashi, H., T. Sakamoto, S. Hyodo, B.S. Shepherd, T. Kaneko and E.G. Grau. 2006. Expression of glucocorticoid receptor in the intestine of a euryhaline teleost, the Mozambique tilapia (Oreochromis mossambicus): Effect of seawater exposure and cortisol treatment. Life Sciences, 78: 2329-2335. https://doi.org/10.1016/j.lfs.2005.09.050
  26. Tomy, S., Y.M. Chang, Y.H. Chen, J.C. Cao, T.P. Wang and C.F. Chang. 2009. Salinity effects on the expression of osmoregulatory genes in the euryhaline black porgy Acanthopagrus schlegeli. Gen. Comp. Endocrinol., 161: 123-132. https://doi.org/10.1016/j.ygcen.2008.12.003
  27. Vijayan, M.M., C.E. Pereira, G. Grau and G.K. Iwama. 1997. Metabolic responses associated with confinement stress in tilapia: the role of cortisol. Comp. Biochem. Physiol. C, 116: 89-95. https://doi.org/10.1016/S0300-9629(96)00156-9
  28. Vijayan, M.M., S. Raptis and R Sathiyaa. 2003. Cortisol treatment affects glucocorticoid receptor and glucocortiocoid-responsive genes in the liver of rainbow trout. Gen. Comp. Endocrinol., 132: 256-263. https://doi.org/10.1016/S0016-6480(03)00092-3
  29. Vizzini, A., M. Vazzana, M. Cammarata and N. Parrinello. 2007. Peritoneal cavity phagocytes from the teleost sea bass express a glucocorticoid receptor (cloned and sequenced) involved in genomic modulation of the in vitro chemiluminescence response to zymosan. Gen. Comp. Endocrinol., 150: 114-123. https://doi.org/10.1016/j.ygcen.2006.07.016
  30. Wedemeyer, G. 1973. Some physiological aspects of sublethal heat stress in the juvenile steelhead trout (Salmo gairdneri) and coho salmon (Oncorhynchus kisutch). J. Fish. Res. Bd. Can., 30: 831-834. https://doi.org/10.1139/f73-138
  31. Wendelaar Bonga, S.E. 1993. Endocrinology. In: Evans, D.H. (ed.), The Physiology of Fishes. CRC Press Inc., Boca Raton, pp. 469-502.
  32. Wendelaar Bonga, S.E. 1997. The stress response in fish. Physiol. Rev., 77: 591-625. https://doi.org/10.1152/physrev.1997.77.3.591