Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Simultaneous Determination of (-)-Menthone and (-)-Menthol in Menthae Herba by Gas Chromatography and Principal Component Analysis

  • Kim, Jung-Hoon (Herbal Medicine EBM Research Center, Standard Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Herbal Medicine EBM Research Center, Standard Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Standard Research Division, Korea Institute of Oriental Medicine)
  • Received : 2010.07.26
  • Accepted : 2010.09.13
  • Published : 2010.09.30
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Abstract

The simple and accurate method was established for the simultaneous determination of (-)-menthone and (-)-menthol in Menthae herba obtained from Korea and China. A quantitative analysis was performed with a gas chromatography-flame ionization detector and reference compounds were separated on a capillary HP-Innowax column (30 m $\times$ 0.23 mm, 0.50 ${\mu}m$, Agilent, MA, USA). The correlation coefficients of the compounds showed good linearity ($r^2$ > 0.9997) over the linear range. The precision, repeatability and stability showed less than 1.7% of relative standard deviation (RSD) values for two compounds. Recovery rates were within the range of 95.72 - 103.76%. The method was applied successfully to analyze 15 samples of Menthae herba and achieved sufficient and specific separation of reference compounds. The principal component analysis (PCA) exhibited the classification of 15 samples according to their locations of origin.

Keywords

References

  1. Gherman, C., Culea, M., and Cozar, O., Comparative analysis of some active principles of herb plants by GC/MS. Talanta 53, 253-262 (2000). https://doi.org/10.1016/S0039-9140(00)00458-6
  2. Goren, A., Topçu, G., Bilsel, G., Bilsel, M., Aydo muþ, Z., and Pezzuto, J.M., The chemical constituents and biological activity of essential oil of Lavandula stoechas ssp. stoechas. Z. Naturforsch C. 57, 797-800 (2002).
  3. Ligor, M. and Buszewski, B., Determination of menthol and menthone in food and pharmaceutical products by solid-phase microextraction-gas chromatography. J. Chromatogr. A 847, 161-169 (1999).
  4. Lin, R., Tian, J., Huang, G., Li, T., and Li, F., Analysis of menthol in three traditional Chinese medicinal herbs and their compound formulation by GC-MS. Biomed. Chromatogr. 16, 229-233 (2002). https://doi.org/10.1002/bmc.131
  5. National Pharmacopoeia Commission of the People's Republic of China, Pharmacopoeia of the People's Republic of China, The Chemical Industry Press, Beijing, 261, 2005.
  6. Nozal, M.J., Bernal, J.L., Jimenez, J.J., Gonzalez, M.J., and Higes, M., Extraction of thymol, eucalyptol, menthol, and camphor residues from honey and beeswax : Determination by gas chromatography with flame ionization detection. J. Chromatogr: A 954, 207-215 (2002). https://doi.org/10.1016/S0021-9673(02)00153-X
  7. Singh, A.K., Raina, V.K., Naqvi, A.A., Patra, N.K., Kumar, B., Ram, P., and Khanuja, S.P.S., Essential oil composition and chemoarrays of menthol mint (Mentha arvensis L. F. piperascens Malinvaud ex. Holmes) cultivars. Flavour. Frag. J. 20, 302-305 (2005). https://doi.org/10.1002/ffj.1417
  8. Sokovi , M.D., Vukojevi , J., Marin, P.D., Brki , D.D., Vajs, V., and Griensven, L.J.V., Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities. Molecules 14, 238- 249 (2009). https://doi.org/10.3390/molecules14010238
  9. South Korea college of pharmacy conference, Pharmacopoeia person and society, Korean Pharmacopeia, Shinilbooks, Seoul, 1123, 2007.
  10. Valdez, J.S., Martin, D.K., and Mayersohn, M., Sensitive and selective gas chromatographic methods for the quantitation of camphor, menthol and methyl salicylate from human plasma. J. Chromatogr. B 729, 163-171 (1999).