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Enhancement of Anticarcinogenic Potentials of Submerged-Liquid Culture of Agaricus blazei Murill on Mouse Ascites Cancer by Rice Hull

왕겨에 의한 신령버섯균사체 액체배양액의 생쥐 항복수암성 증가

  • Kim, Young-S. (Division of Applied Life Science (BK21 programs)) ;
  • Jang, Wook-J. (Division of Applied Life Science (BK21 programs)) ;
  • Rakib, A. (Division of Applied Life Science (BK21 programs)) ;
  • Kwon, Jung-M. (Division of Applied Life Science (BK21 programs)) ;
  • Ahn, Chae-R. (Division of Applied Life Science (BK21 programs)) ;
  • Kim, So-Y. (Department of Food Science, International University of Korea) ;
  • Cho, Yong-U. (Department of Pharmaceutical Engineering, Jinju National University) ;
  • Ha, Young-K. (HK Biotech. Co., Ltd.) ;
  • Kim, Jeong-O. (HK Biotech. Co., Ltd.) ;
  • Ha, Yeong-L. (Division of Applied Life Science (BK21 programs))
  • 김영숙 (경상대학교 응용생명과학부(BK21)) ;
  • 장욱진 (경상대학교 응용생명과학부(BK21)) ;
  • 라키브 (경상대학교 응용생명과학부(BK21)) ;
  • 권정민 (경상대학교 응용생명과학부(BK21)) ;
  • 안채린 (경상대학교 응용생명과학부(BK21)) ;
  • 김소영 (한국국제대학교 식품과학과) ;
  • 조용운 (진주산업대학교 제약공학과) ;
  • 하영권 ((주) HK바이오텍) ;
  • 김정옥 ((주) HK바이오텍) ;
  • 하영래 (경상대학교 응용생명과학부(BK21))
  • Received : 2010.07.26
  • Accepted : 2010.08.27
  • Published : 2010.09.30

Abstract

The effects of rice hull (RH) powder on the anticarcinogenic activity of submerged-liquid cultures of Agaricus blazei Murill (AB) were assessed for mouse ascites cancers induced by mouse Sarcoma S-180 (S-180) cancer cells. Optimal growth of AB mycelia in the basal liquid culture medium, containing soybean meal, was achieved by culturing at $25^{\circ}C$ for 5 days, when evaluated by $\beta$-glucan content, Brix, and mycelial weight, relative to other culture conditions. Hot-water extract (HWE) of the submergedliquid culture of AB mycelia grown at $25^{\circ}C$ for 5 days exhibited a stronger anticarcinogenic activity, relative to HWE from other culture conditions. No such effects were obtained from AB mycelial cultures by alternative temperature-controlling cultures. Both cytotoxicity for S-180 cells and anticarcinogenic potentials for mouse ascites cancer of the HWE from AB mycelia grown in the basal medium containing 1% RH powder for 5 days at $25^{\circ}C$ were significantly (p<0.05) enhanced, relative to HWE from the AB mycelia culture of the basal medium without RH powder. These results indicate that HWE of submerged-liquid culture of AB mycelia, incubated in media containing 1% RH powder at $25^{\circ}C$ for 5 days, enhanced anticarcinogenic activity against S-180 cell-induced mouse ascites cancer, and suggest that RH powder is an excellent ingredient for the improvement of the anticarcinogenic potentials of the submerged-liquid culture of mushroom mycelia.

왕겨가 Agaricus blazei Murill (AB: 신령버섯)균사체 액체배양 추출물의 항암성을 증가시키는지에 관한 연구를 수행하였다. AB균사체를 대두박을 기본으로 한 액체배지에 다양한 조건으로 배양하여, $\beta$-glucan 함량, Brix, 균사체를 측정하여 적정 생육조건을 선정하고, 이들의 열수추출물의 S-180 cell로 유도한 mouse 복수암에 대한 항암성을 조사하였다. AB균사체는 $25^{\circ}C$에서 5일간 배양하였을 때 최적 생육을 나타내었고, 이 배양물이 다른 조건에서 배양한 배양물보다 우수한 항암성을 나타내었다. AB균사체의 생육 및 항암성은 변온배양에 따른 효과는 없었다. 따라서 이 최적배양조건($25^{\circ}C$, 5일 배양)에서 AB균사체를 1% 왕겨분말이 함유된 액체배지에 배양하고, 이의 열수 추출물의 항암성을 검증하였다. 1% 왕겨가 함유된 액체배지에서 배양한 열수추출물의 항암성은 왕겨가 함유되지 않은 배지의 열수추출물보다 항암성이 유의성 있게 증가되었다(p<0.05). 왕겨의 첨가는 AB균사체의 생육을 오히려 촉진시켰다. 이 결과는 왕겨가 AB균사체 뿐 만 아니라 다른 버섯균사체 액체배양물의 항암성 증진을 위한 원료로 활용될 수 있을 것임을 의미한다.

Keywords

References

  1. Bahn, K. N., E. J. Lee, M. S. Yang, J. O. Kim, and Y. L. Ha. 1995. Potent anticarcinogenic action of Moutan radix for mouse ascites cancer induced by mouse Sarcoma 180 cells. Agric. Chem. Biotechnol. 38, 364-369.
  2. Chang, H. L., G. R. Chao, C. C. Chen, and J. L. Mau. 2001. Non-volatile taste components of Agaricus blazei, Antrodia camphorata and Cordyceps militaris mycelia. Food Chem. 74, 203-207. https://doi.org/10.1016/S0308-8146(01)00127-3
  3. Choi, J. M. and S. J. Koo. 2000. Effects of $\beta$-glucan from Agaricus blazei Murill on blood glucose and lipid composition in db/db mice. Korean J. Food Sci. Technol. 32, 1418-1425.
  4. Choi, H. C. and S. J. Park. 2003. Element compositions of rice hull and rice husk ash in Korea. Korean J. Nutri. 36, 352-358.
  5. Dong, Q., J. Yao, X. T. Yang, and J. N. Fang. 2002. Structural characterization of water-soluble $\beta$-D-glucan from fruiting bodies of Agaricus blazei Murr. Carbohydrate Res. 337, 417-1421.
  6. Franz, G. 1989. Polysaccharides in pharmacy: current applications and future concepts. Planta Med. 55, 493-497. https://doi.org/10.1055/s-2006-962078
  7. Itoh, H., H. Amano, and H. Noda. 1994. Inhibitory action of a (1→6)-$\beta$-D-glucan-protein complex isolated from Agaricus blazei Murill on metha fibrosarcoma-bearing mice and its antitumor mechanism. Jpn. J. Pharmacol. 66, 265-271. https://doi.org/10.1254/jjp.66.265
  8. Jhune, C. S., G. P. Kim, and C. W. Shin. 2000. Effect of rice bran added at spawn-making on the cultivation of Oyster mushroom, Pleurotus spp. Korean J. Mycology 28, 1-5.
  9. Jung, I. C., S. H. Kim, Y. I. Kwon, and J. S. Lee. 1996. Cultural Condition for the mycelial growth of Ganoderma lucidum on cereals. Korean J. Mycol. 24, 81-88.
  10. Kang, A. S., T. S. Kang, S. M. Cho, and S. H. Yu. 2001. Studies on Submerged culture and mycelial components of Naematoloma sublateritium mycelia. Korean J. Mycol. 29, 22-27.
  11. Kawagish, H., R. Inagaki, and T. Kanao. 1989. Fraction and antitumor activity of the water- insoluble residue of Agaricus blazei fruiting bodies. Carbohydrate Res. 186, 267-273. https://doi.org/10.1016/0008-6215(89)84040-6
  12. Kawagish, H., T. Kanao, R. Inagaki, T. Mizuno, K. Shimura, H. Ito, T. Hagiwara, and T. Nkamura. 1990. Formolysis of a potent antitumor (l-6)-$\beta$-D-glucan-protein complex from Agaricus blazei fruiting bodies and antitumor activity of the resulting products. Carbohydrate Polymers 12, 393-403. https://doi.org/10.1016/0144-8617(90)90089-B
  13. Kim, S. W., H. J. Hwang, J. P. Park, Y. J. Cho, C. H. Sang, and J. W. Yun. 2002. Mycelial growth and exo-biopolymer production by submerged culture of various edible mushrooms under different media. Lett. Microbiol. 34, 56-61. https://doi.org/10.1046/j.1472-765x.2002.01041.x
  14. Kim, S. J., H. R. Park, E. J. Park, and S. C. Lee. 2007. Cytotoxic and antitumor Activity of momilactone B from rice hulls. J. Agric. Food Chem. 55, 1702-1706. https://doi.org/10.1021/jf062020b
  15. Kuko, K. and H. Nanba. 1996. The effect of Maitake mushrooms on liver and serum lipids. Altern. Ther. Health Med. 2, 62-69.
  16. Kumagai, S., N. Hayashi, T. Sakaki, M. Nakada, and M. Shibata. 2004. Fractionation and saccharification of cellulose and hemicellulose in rice hull by hot-compressed-water treatment with two-step heating. J. Japan Inst. Energy 83, 776-78. https://doi.org/10.3775/jie.83.776
  17. Lin, J. H. and S. S. Yang. 2006. Mycelium and polysaccharide production of Agaricus blzei Murrill by submerged fermentation. J. Microbiol. immunol. Infect. 39, 98-108.
  18. Liu, F., V. E. Ooi, and S. T. Chang. 1997. Free radical scanvenging activities of mushroom polysaccharide extracts. Life Sci. 60, 763-766. https://doi.org/10.1016/S0024-3205(97)00004-0
  19. Menoli, R. C. R. N., M. S. Mantovani, L. R. Ribeiro, G. Speit, and B. Q. Jordao. 2001. Antimutagenic effects of the mushroom Agaricus blazei Murill extracts on V79 cells. Mut. Res. 496, 5-13. https://doi.org/10.1016/S1383-5718(01)00226-1
  20. Mizuno, M., M. Morimoto, K. Minate, and H. Tsucjida. 1998. Polysaccharides from Agaricus blasei stimulate lymphocyte T-cell subsets in mice. Biosci. Biotechnol. Biochem. 62, 434-437. https://doi.org/10.1271/bbb.62.434
  21. Mizuno, T., T. Hagiwara, T. Nakamura, H. Ito, K. Shimura, and T. Sumiya. 1990. Antitumor activity and some properties of water soluble polysaccharides from the fruiting body of Agaricus blazei Murill, Agric. Biol. Chem. 54, 2889-2896. https://doi.org/10.1271/bbb1961.54.2889
  22. Nakajima, A., T. Ishida, M. Koga, and M. Takeuchi. 2002. Effect of hot water extract from Agaricus blasei Murill on antibody-producing cells in mice. Int. Immunopharmacol. 2, 1205-1211. https://doi.org/10.1016/S1567-5769(02)00056-5
  23. Park, K. S., J. E. Son, and G. H. Yoon. 2001. Effects of low and alternated temperature treatments on quality of oak mushroom in sawdust. J. Bio-Environ. Control 10, 3-10.
  24. Smith, J. E., N. J. Rowan, and S. Richard. 2002. Medicinal mushrooms: a ripidly developing area of biotechnology for cancer therapy and other bioactivities. Biotech. Letter 24, 1839-1845. https://doi.org/10.1023/A:1020994628109
  25. Sorensen, H. R., A. S. Meyer, and S. Pedersen. 2003. Enzymatic hydrolysis of water-soluble wheat arabinoxylan. 1. Synergy between L-arabinofuranosidases, endo-1,4--xylanases, and xylosidase activities. Biotechnol. Bioeng. 81, 726-731. https://doi.org/10.1002/bit.10519
  26. Sugiyama, K., S. Saeki, and Y. Ishguro. 1992. Hypercholesterolemic activity of ningyotake (Polyporus confleuens) mushroom in rats. J. Jpn. Soc. Nutr. Food Sci. 45, 265-270. https://doi.org/10.4327/jsnfs.45.265
  27. Sung, J. M., Y. B. Yoo, and D. Y. Cha. 1998. Mushroom. pp. 3-10, Kyohaksa. Seoul.
  28. Wasser, S. P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol. 60, 258-274. https://doi.org/10.1007/s00253-002-1076-7
  29. Watanabe, T., M. Shida, Y. Furuyama, K. Tsukamoto, T. Nakajima, and K. Matsuda. 1983. Structure of the arabinoxylan of rice hull. Carbohydrate Res. 123, 83-95. https://doi.org/10.1016/0008-6215(83)88383-9
  30. Yoshiaki F, K. Hidekazu, O. Koichi, S. Ryo, E. Takusaburo. 1998. Tumoricidal activity of high molecular weight polysaccharides derived from Agaricus blazei via oral administration in the mouse tumor model. Kagaku Kaishi 45, 246-252.

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