Effects of Horse Bone Extracts on the Induced Postmenopausal Osteoporosis in Rats

백서의 폐경기 골다공증 모델에서 말뼈 추출물의 효과

  • Park, Sun-Soon (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Lee, Hye-Ja (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Yoon, Weon-Jong (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Kang, Gyeoung-Jin (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Yang, Eun-Jin (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Kim, Hyo-Sun (JEJU BIO WORLD) ;
  • Choo, Chang-Su (JEJU BIO WORLD) ;
  • Kang, Hee-Kyoung (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Yoo, Eun-Sook (Department of Pharmacology, College of Medicine, Jeju National University)
  • 박선순 (제주대학교 의과대학 약리학교실) ;
  • 이혜자 (제주대학교 의과대학 약리학교실) ;
  • 윤원종 (제주대학교 의과대학 약리학교실) ;
  • 강경진 (제주대학교 의과대학 약리학교실) ;
  • 양은진 (제주대학교 의과대학 약리학교실) ;
  • 김효선 (제주바이오세상) ;
  • 추창수 (제주바이오세상) ;
  • 강희경 (제주대학교 의과대학 약리학교실) ;
  • 유은숙 (제주대학교 의과대학 약리학교실)
  • Received : 2010.07.06
  • Accepted : 2010.08.06
  • Published : 2010.09.30

Abstract

Osteoporosis is a metabolic bone disease associated with an imbalance of bone remodeling. Osteoporosis is characterized by decreased bone mass and increased bone fractures. In this study, we investigated the effects of horse bone extracts (HBEs) in vivo. Horse bone was extracted with 80% alcohol (HBE-A) at $100^{\circ}C$ or water (HBE-W) at $120^{\circ}C$. Animal model of postmenopausal osteoporosis was used, in which osteoporosis was induced by ovariectomy of female S.D. rats (female rats were divided into 5 groups), and HBEs were administered to ovariectomized rats every day for 8 weeks. After 8 weeks, the rats were sacrificed and the following osteoporotic factors were measured: body weight, bone mineral density (BMD), uterine/body weight ratio, serum estradiol (E2), and serum alkaline phosphatase (ALP). The results showed that the administration of HBE-W decreased the changes of body weight in ovariectomized rats. HBE-W increased the uterine/body weight ratio and BMD. In addition, HBEs decreased the ALP. Therefore, HBEs may be used for the prevention or treatment of bone disease.

Keywords

References

  1. 배용철 외 23 명 (역) (2005) 핵심조직학 2판, 134-154. 범문사, 서울.
  2. 한국임상병리교수협의회 세포분과위원회 (2004) 조직학 (Histology), 105-115. 고려의학, 서울.
  3. Hobson, E. E. and Ralsteo, S. H. (2001) Role of genetic factors in the pathophysiology and management of osteoporosis. Clin. Endocrinlo. (Oxf). 54: 1-9. https://doi.org/10.1046/j.1365-2265.2001.01149.x
  4. Garnero, P. and Delmas, P. D. (1996) New developments in biochemical markers for osteoporosis. Calcif. Tissue Int. 1: S2-S9.
  5. Johansen, J. S., Riis, B. J., Delmas, P. D. and Christiansen, C. (1988) Plasma BGP: an indicator of spontaneous bone loss and of the effect of oestrogen treatment in postmenopausal women. Eur. J. Clin. Invest. 18: 191-195. https://doi.org/10.1111/j.1365-2362.1988.tb02412.x
  6. Cauley, J. A., Gutai, J. P., Sandier, R. B., LaPorte, A. E., Kuller, L. H. and Sashin, D. (1986) The relationship of endogenous estrogen to bone density and bone area in normal poetmenopausal women. Am. J. Epldemiol. 124: 752-761. https://doi.org/10.1093/oxfordjournals.aje.a114451
  7. Ohta, H., Masuzawa, T., Ikeda, T., Suda, Y., Makita, K. and Nozawa, S. (1992) Which is more osteoporosis inducing, menopause or oophorectomy? Bone Miner. 19: 273-285. https://doi.org/10.1016/0169-6009(92)90876-F
  8. Li, M., Shen, Y. and Wronski, T. J. (1997) Time course of femoral neck osteopenia in ovariectomized rats. Bone. 20: 55-61. https://doi.org/10.1016/S8756-3282(96)00317-1
  9. 이대일, 최종상 (1997) 개정증보 병리학개론 3판, 270-288. 신광출판사, 서울.
  10. 전국 의과대학 교수편 (역) (2002) 임상약리학, 771-789. 대한의학서적, 서울.
  11. Anderson, J. J. and Garner, S. C. (1998) Phytoestrogens and bone. Baillieres Clin. Endocrinol. Metab. 12: 543-557. https://doi.org/10.1016/S0950-351X(98)80003-7
  12. Setchell, K. D. and Cassidy, A. (1999) Dietary isoflavones: biological effects and relevance to human health. J. Nutr. 129: 758S-767S. https://doi.org/10.1093/jn/129.3.758S
  13. Lee, C. J., Lawler, G. S. and Johnson, G. H. (1981) Effects of supplementation of the diets with calcium and calcium-rich foods on bone density of elderly females with osteoporosis. Am. J. Clin. Nutr. 34: 819-823. https://doi.org/10.1093/ajcn/34.5.819
  14. Reid, I. R. and Ibbertson, H. K. (1986) Calcium supplements in the prevention of steroid-induced osteoporosis. Am. J. Clin. Nutr. 44: 287-290. https://doi.org/10.1093/ajcn/44.2.287
  15. Freudenheim, J. L., Johnson, N. E. and Smith, E. L. (1986) Relationships between usual nutrient intake and bone-mineral content of women 35-65 years of age: longitudinal and cross-sectional analysis. Am. J. Clin. Nutr. 44: 863-876. https://doi.org/10.1093/ajcn/44.6.863
  16. Tylavsky, F. A. and Anderson, J. J. (1988) Dietary factors in bone health of elderly lactoovovegetarian and omnivorous women. Am. J. Clin. Nutr. 48: 842-849. https://doi.org/10.1093/ajcn/48.3.842
  17. Koo, J. O., Kwak, C. S. and Choi, H. M. (1991) Effects of dietary protein levels and sources on calcium and phosphorus metabolism in young Korean women. Korean J. Nutr. 24: 124-131.
  18. Calvo, M. S. (1994) The effects of high phosphorus intake on calcium homeostasis. Adv. Mutr. Res. 9: 183-207.
  19. Jeong, H. K., Kim, J. Y., Lee, H. S. and Kim, J. Y. (1997) The effect of dietary calcium and phosphate levels on calcium and bone metabolism in rats. Korean J. Nutr. 30: 813-824.
  20. Rucker, R. B., Parker, H. E. and Rogler, J. C. (1969) Effect of copper deficiency on chick bone collagen and selected bone enzymes. J. Nutr. 98: 57-63. https://doi.org/10.1093/jn/98.1.57
  21. Kalu, D. N. (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner. 15: 175-191. https://doi.org/10.1016/0169-6009(91)90124-I
  22. Wronski, T. J., Yen, C. F., Qi, H. and Dann, L. M. (1993) Parathyroid hormone is more effective than estrogen or bisphosphonates for restoration of lost bone mass in ovariectomized rats. Endocrinology. 132: 823-831. https://doi.org/10.1210/en.132.2.823
  23. Faugere, M. C., Okamoto, S., DeLuca, H. F. and Malluche, H. H. (1986) Calcitriol corrects bone loss induced by oophorectomy in rats. Am. J. Physiol. 250: E35-E38.
  24. Mueller, K. and Hsiao, S. (1980) Estrus- and ovariectomyinduced body weight changes: evidence for two estrogenic mechanisms. J. Comp. Physiol. Psychol. 94: 1126-1134. https://doi.org/10.1037/h0077746
  25. Malluche, H. H., Sherman, D., Meyer, W. and Massry, S. G. (1982) A new semiautomatic method for quantitative static and dynamic bone histology. Calcified Tissue International. 34: 439-448. https://doi.org/10.1007/BF02411282
  26. Bagi, C. M., Ammann, P., Rizzoli, R. and Miller, S. C. (1997) Effect of estrogen deficiency on cancellous and cortical bone structure and strength of the femoral neck in rats. Calcif. Tissue Int. 61: 336-344. https://doi.org/10.1007/s002239900344
  27. Durbridge, T. C., Morris, H. A., Parsons, A. M., Parkinson, I. H., Moore, R. J., Porter, S., Need, A. G., Nordin, B. E. C. and Vernon-Roberts, B. (1990) Progressive cancellous bone loss in rats after adrenalectomy and oophorectomy. Calcif. Tissue Int. 47: 383-387. https://doi.org/10.1007/BF02555891
  28. Kim, J. S. (2003) Research on the raising of horses and its symbolic status in the ancient Shilla society. 韓國史硏究 123: 21-53.
  29. 허준 (1995) 동의보감, 489. 도서출판삼성문화사, 서울.
  30. 이종언 (2006) 말고기와 말뼈의 영양 특성. 난지농업연구지 15: 20-22.
  31. Seong, P. N., Lee, C. E., Kim, J. H., Cho, S. H. Hah, K. H., Lim, D. G., Kim, D. H., Lee, J. M. and Ko, M. S. (2008) Effect of horse meat content on the quality and sensory characteristics of Press Ham. Korean J. Food Sci. Ani. Resour. 28: 9-13. https://doi.org/10.5851/kosfa.2008.28.1.9
  32. Kim, Y. B., Jeon, K. H., Rho, J. H. and Kang, S. N. (2005) Physicochemiacl properties of Loin and Rump in the native horse meat from Jeju. Korean J. Food Sci. Ani. Resour. 25: 365-372.
  33. 한국식품공업협회 (2006) 식품공전, 583-610, 941-943. 주식회사 문영사, 서울.
  34. Choi, H. J., Kim, E. J., Kim, N. J. and Kim, S. S. (2006) Studies on pharmaceutical quality of Oriental medicinal preparations (I) - Studies on decoction of Nokyong-Sagunja-Tang. Korean Journal of Pharmacognosy 37: 143-150.
  35. Kim, S. K., Ban, S. Y., Kim, J. S. and Chung, S. K. (2005) Change of antioxidant activity and antioxidant compounds in Saururus chinensis by extraction conditions. J. Korean Soc. Appl. Biol. Chem. 48: 89-92.
  36. Miller, P. D., Bonnick, S. L., Johnston, C. C., Kleerekoper, M., Lindsay, R. L., Sherwood, L. M. and Siris, E. S. (1998) The challenges of peripheral bone density testing: which patients need additional central density skeletal measurements? J. Clin. Densitom. 1: 211-217. https://doi.org/10.1385/JCD:1:3:211
  37. Shen, V., Birchman, R., Xu, R., Lindsay, R. and Dempster, D. W. (1995) Short term changes in histomorphometric and biochemical turnover markers and bone mineral density in estrogen and/or dietary calcium deficient rats. Bone. 16s 149-156.
  38. Lee, H. M., Hahn, S. B. and Park, B. M. (1992) Effect of calcitonin on osteoporosis induced by ovariectomy in rats. J. Korean Orthop. Assoc. 27: 577-582.
  39. Eastell, R., Delmas, P. D., Hodgson, S. F., Eriksen, E. F., Mann, K. G. and Riggs, B. L. (1988) Bone formation rate in older normal women: concurrent assessment with bone histomorphometry, calcium kinetics, and biochemical markers. J. Clin. Endocrinol. Metab. 67: 741-748. https://doi.org/10.1210/jcem-67-4-741
  40. Duda, R. J. Jr., O'Brien, J. F., Katzmann, J. A., Peterson, J. M., Mann, K. G. and Riggs, B. L. (1988) Concurrent assays of circulating bone Gla-protein and bone alkaline phosphatase: effect of sex, age, and metabolic bone disease. J. Clin. Endocrinol. Metab. 66: 951-957. https://doi.org/10.1210/jcem-66-5-951