Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Design and Synthesis of Metallopeptide Sensors: Tuning Selectivity with Ligand Variation

  • Kim, Joung-Min (Bioorganic Chemistry Lab, Department of Chemistry, Inha University) ;
  • Joshi, Bishnu Prasad (Bioorganic Chemistry Lab, Department of Chemistry, Inha University) ;
  • Lee, Keun-Hyeung (Bioorganic Chemistry Lab, Department of Chemistry, Inha University)
  • Received : 2010.07.06
  • Accepted : 2010.07.19
  • Published : 2010.09.20
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Abstract

We chose a fluorescent pentapeptide sensor (-CPGHE) containing a dansyl fluorophore as a model peptide and investigated whether the selectivity and sensitivity of the peptides for heavy and transition metal ions could be tuned by changing amino acid sequence. In this process, we developed a selective peptide sensor, Cp1-d (-HHPGE, $K_d\;=\;670\;nM$) for detection of $Zn^{2+}$ in 100% aqueous solution and a selective and sensitive peptide sensor, Cp1-e (-CCHPGE, $K_d\;=\;24\;nM$) for detection of $Cd^{2+}$ in 100% aqueous solution. Overall results indicate that the selectivity and sensitivity of the metallopeptide sensors to specific heavy and transition metal ions can be tuned by changing amino acid sequence.

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References

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