Journal of Plant Biotechnology

  • 제37권3호
  • /
  • Pages.243-249
  • /
  • 2010
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

분자농업의 현황 및 전망

Current status in molecular farming

  • 김태금 (전북대학교 자연과학대학 분자생물학과) ;
  • 양문식 (전북대학교 자연과학대학 분자생물학과)
  • Kim, Tae-Geum (Department of Molecular Biology, Chonbuk National University, Jeonju Center, Korea Basic Science Institute) ;
  • Yang, Moon-Sik (Department of Molecular Biology, Chonbuk National University, Jeonju Center, Korea Basic Science Institute)
  • 투고 : 2010.08.02
  • 심사 : 2010.08.16
  • 발행 : 2010.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Molecular farming is production of pharmaceutically and industrially important proteins in plants. Plants and plant cell culture systems have been used as bio-factory to produce recombinant proteins such as monoclonal antibodies, enzymes, vaccines, hormones, interleukins, commercial enzymes and etc. The terms molecular farming, biofarming, molecular pharming, phytomanufacturing, recombinant or plant-made industrials, planta-pharma, plant bioreactors, plant biofactory, and pharmaceutical gardening are used interchangeably. Molecular farming can provide safe and inexpensive pharmaceutical proteins as well as commercial ones. In spite of several advantages of molecular farming such as safety and inexpensive cost, there are also a couple of drawbacks in the existing technology. One of them is low expression level of target gene in plants, which has been improved by optimizing gene-based codon usage, screening of strong promoters, expression of transcription factors, subcellular targeting of target proteins, chloroplast transformation, and transient expression using viral expression system (magnifection). Some plant-based commercial proteins have already been in markets and more than twenty plant-based pharmaceuticals have been in clinical trials, from that we can expect that several plant-based pharmaceutical proteins will be seen in the markets in the near future.

키워드

참고문헌

  1. Bae JL, Lee JG, Kang TJ, Jang HS, Jang YS, Yang MS (2003) Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing the antigen. Vaccine 8:4052-4058
  2. Barta A, Sommergruber K, Thompson D, Hartmuth K, Matzke M, Matzke A (1986) The expression of a nopaline synthase human growth hormone chimeric gene in transformed tobacco and sunflower callus tissue. Plant Mol Biol 6:347-357 https://doi.org/10.1007/BF00034942
  3. Basaran P, Rodriguez-Cerezo E (2008) Plant molecular farming: Opportunities and challenges. Crit Rev Biotechnol 28:153-172 https://doi.org/10.1080/07388550802046624
  4. Bock R (2001) Transgenic plastids in basic research and plant biotechnology. J Mol Biol 312:425-438 https://doi.org/10.1006/jmbi.2001.4960
  5. Commandeur U, Twyman RM, Fischer R (2003) The biosafety of molecular farming in plants. AgBiotechNet 30:109-112
  6. Daniell H, Chebolu S, Kumar S, Singleton M, Falconer R (2005) Chloroplast-derived vaccine antigens and other therapeutic proteins. Vaccine 23:1779-1783 https://doi.org/10.1016/j.vaccine.2004.11.004
  7. De Jaeger G, Scheffer S, Jacobs A, Zambre M, Zobell O, Goossens A, Angenon G (2002) Boosting heterologous protein production in transgenic dicotylodonous seeds using Phaseolus vulgaris regulatory sequences. Nat Biotechnol 20:1265-1268 https://doi.org/10.1038/nbt755
  8. De Zoeten GA, Penswick JR, Horisberger MA, Ahl P, Schultze M, Hohn T (1989) The expression, localization, and effect of a human interferon in plants. Virology 172:213-222 https://doi.org/10.1016/0042-6822(89)90123-2
  9. Doran PM (2006) Foreign protein degradation and instability in plants and plant tissue cultures. Trends Biotechnol 24:426-432 https://doi.org/10.1016/j.tibtech.2006.06.012
  10. Gleba Y, Klimyuk V, Marillonnet S (2007) Magnifection-a new platform for expressing recombinant vaccines in plants. Vaccine 23:2042-2048 https://doi.org/10.1016/j.vaccine.2005.01.006
  11. Haq TA, Mason HS, Clements JD, Arntzen CJ (1995) Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 268:714-716 https://doi.org/10.1126/science.7732379
  12. Hiatt A, Cafferkey R, Bowdish K (1989) Production of antibodies in transgenic plants. Nature 342:76-78 https://doi.org/10.1038/342076a0
  13. Horn ME, Woodard SL, Howard JA (2004) Plant molecular farming: systems and products. Plant Cell Rep 22:711-720 https://doi.org/10.1007/s00299-004-0767-1
  14. Ibrahim AF, Watters JA, Clark GP, Thomas CJ, Brown JW, Simpson CG (2001) Expression of intron-containing GUS constructs is reduced due to activation of a crytic 5' splice site. Mol Genet Genomics 265:455-460 https://doi.org/10.1007/s004380000433
  15. Kang TJ, Han SC, Jang MO, Kang KH, Jang YS, Yang MS (2004) Enhanced expression of B-subunit of Escherichia coli heatlabile enterotoxin in tobacco by optimization of coding sequence. Appl Biochem Biotechnol 117:175-187 https://doi.org/10.1385/ABAB:117:3:175
  16. Kang TJ, Kim YS, Jang YS, Yang MS (2005) Expression of the synthetic neutralizing epitope gene of porcine epidemic diarrhea virus in tobacco plants without nicotine. Vaccine 23:2294-2297 https://doi.org/10.1016/j.vaccine.2005.01.027
  17. Karg SR, Kallio PT (2009) The production of biopharmaceuticals in plant systems. Biotechnol Adv 27:879-894 https://doi.org/10.1016/j.biotechadv.2009.07.002
  18. Kim TG, Kim BG, Kim MY, Choi JK, Jung ES, Yang MS (2009) Expression and immunogenicity of enterotoxigenic Escherichia coli heat-labile toxin B subunit in transgenic rice callus. Mol Biotechnol 44:14-21
  19. Kim YS, Kim MY, Kim TG, Yang MS (2009) Expression and assembly of cholera toxin B subunit (CTB) in transgenic carrot (Daucus carota L.). Mol Biotechnol 41:8-14 https://doi.org/10.1007/s12033-008-9086-z
  20. Kumar GB, Ganapathi TR, Revathi CJ, Bapat VA (2005) Expression of hepatitis B surface antigen in transgenic banana plants. Planta 222:484-493 https://doi.org/10.1007/s00425-005-1556-y
  21. Lee KY, Kim DH, Kang TJ, Kim J, Jung GH, Yoo HS, Arntzen CJ, Yang MS, Jang YS (2006) Induction of protective immune responses against the challenge of Actinobacillus pleuropneumoniae by the oral administration of transgenic tobacco plant expressing ApxIIA toxin from the bacteria. FEMS Immunol Med Microbiol 48:381-389 https://doi.org/10.1111/j.1574-695X.2006.00158.x
  22. Mason HS, Haq TA, Clements JD, Arntzen CJ (1998) Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene. Vaccine 16:1336-1343 https://doi.org/10.1016/S0264-410X(98)80020-0
  23. Outchkourov NS, Peters J, de Jong J, Rademakers W, Jongsma MA (2003) The promoter-terminator of chrysanthemum rbsS1 directs very high expression levels in plants. Planta 216:1003-1012
  24. Poirier Y, Dennis DE, Klomparens K, Somerville C (1992) Polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants. Science 256:520-523 https://doi.org/10.1126/science.256.5056.520
  25. Schillberg S, Twyman RM, Fischer R (2005) Opportunities for recombinant antigen and antibody expression in transgenic plants technology assessment. Vaccine 23:1764-1769 https://doi.org/10.1016/j.vaccine.2004.11.002
  26. Shin YJ, Hong SY, Kwon TH, Jang YS, Yang MS (2002) High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture. Biotechnol Bioeng 82:778-783 https://doi.org/10.1002/bit.10635
  27. Sijmons PC, Dekker BMM, Schrammeijer B, Verwoerd TC, van den Elzen PJ, Hoekema A (1990) Production of correctly processed human serum albumin in transgenic plants. Bio/Technol 8:217-221 https://doi.org/10.1038/nbt0390-217
  28. Vaquero C, Sack M, Schuster F, Finnern R, Drossard J, Schumann D, Reimann A, Fischer R (2002) A carcinoembryonic antigenspecific diabody produced in tobacco. FASEB J 16:408-410 https://doi.org/10.1096/fj.01-0363fje
  29. Walsh J (1998) Pharmaceuticals, biologics and biopharmaceuticals. In: Biopharmaceuticals: Biochemistry and Biotechnology, (pp. 1-35) Wiley, Chichester, UK
  30. Yu J, Langridge WH (2001) A plant-based multicomponent vaccine protects mice from enteric diseases. Nat Biotechnol 19:548-552 https://doi.org/10.1038/89297
  31. Yang MS (2009) Development and status of plant-based edible vaccine using transgenic plants. BioSafety 10:44-53
  32. Zhou F, Badillo-Corona JA, Karcher D, Gonzalez-Rabade N, Piepenburg K, Borchers AM, Maloney AP, Kavanagh TA, Gray JC, Bock R (2008) High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes. Plant Biotechnol J 6:897-913 https://doi.org/10.1111/j.1467-7652.2008.00356.x
  33. 박기영 (2010) 농림수산식품 신성장 동력으로서 분자농업의 전망과 과제. 농림수산식품기술기획평가원.
  34. http://www.ipet.re.kr/morgu/data_view.asp?seq=13&page=1&column=&keyword=&bo_tpcd=L&func=