The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effects of Bee Venom Herbal Acupuncture on Experimental Rat Model of Benign Prostatic Hyperplasia

봉독약침(蜂毒藥鍼)이 전립선비대증(前立腺肥大症) Rat에 미치는 영향

  • Cho, So-Hyun (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Shin University) ;
  • Han, Yang-Hee (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Shin University) ;
  • Kim, Young-Seong (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Shin University)
  • 조소현 (동신대학교 한의과대학 신계내과학교실) ;
  • 한양희 (동신대학교 한의과대학 신계내과학교실) ;
  • 김용성 (동신대학교 한의과대학 신계내과학교실)
  • Published : 2010.03.31
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Abstract

Objective : Benign prostatic hyperplasia(BPH) is one of the most common diseases among elderly men. In BPH, dihydrotestosterone (DHT) acts as a potent cellular androgen and promotes prostate growth. Many reports conclude the component melittin in bee venom has the potential to treat various diseases including prostate cancer. In this study, we investigated the therapeutic effects and action mechanism of Bee venom herbal acupuncture with BPH induced by castration and testosterone treatment. Methods : Sprague-Dawley rats were treated with testosterone after castration for induction of experimental BPH. A total of 24 rats were equally divided into four groups: Group 1 was the model group; Group 2 served as control (sham-operated group); Group 3 animals were treated with Bee venom herbal acupuncture as an experimental specimen; Group 4 served as a positive control group and was treated with finasteride at a dose of 1 mg/kg. The drugs were administered orally. The prostates were evaluated by prostatic weight, volume, histopathological changes and testosterone levels. Results : While prostates of control rats revealed severe acinar gland atrophy and stromal proliferation, the rats treated with Bee venom herbal acupuncture showed a diminished range of tissue damage and showed significant decrease in their prostatic weights, volume and histopathological examination. Conclusions : These results suggest that Bee venom herbal acupuncture may protect the glandular epithelial cells and also inhibit stromal proliferation. From theses results, we suggest that Bee venom herbal acupuncture could be a useful remedy agent for treating the benign prostatic hyperplasia.

Keywords

References

  1. Apolone G, Cattaneo A, Colombo P, La Vecchia C, Cavazzuti L, Bamfi F. Knowledge and opinion on prostate and prevalence of self-reported BPH and prostate-related events. A cross-sectional survey in Italy. Eur J Cancer Prev. 2002;11(5): 473-9. https://doi.org/10.1097/00008469-200210000-00010
  2. Wasson JH, Reda DJ, Bruskewitz RC, Elinson J, Keller AM, Henderson WG. A comparison of transurethral surgerythral watchful waiting for moderate symptoms of benign prostatic hyperplasia. The Veterans Affairs Cooperative Study a Theon Transurethral Resection of the Prostate. N Engl J Med. 1995;332:75-9. https://doi.org/10.1056/NEJM199501123320202
  3. Caine M. The present role of $\alpha$-adrenergic blockers in the treatment of benign prostatic hypertrophy. J Urol. 1986;136:1-4.
  4. Mebust WK, Holtgrewe HL, Cockett AT, Peters PC. Transurethral prostatec tomy: immediate and postoperative complications. Cooperative study of 13 participating institutions evaluating 3,885 patients. J Urol. 2002;167:5-9. https://doi.org/10.1016/S0022-5347(05)65370-0
  5. Gormley GJ, Stoner E, Bruskewitz RC, Imperato -McGinley J, Walsh PC, McConnell JD, et al. The effect of finasteride in men with benign prostatic hyperplasia. The Finasteride Study Group. N Engl J Med. 1992;327:1185-91. https://doi.org/10.1056/NEJM199210223271701
  6. McConnell JD, Bruskewitz R, Walsh P, Andriole G, Lieber M, Holtgrewe HL, et al. The effect of finasteride on the risk of acute urinary retention and the need for surgical treatment among men with benign prostatic hyperplasia. Finasteride Long -term Efficancy and Safety Study Group. N Engl J Med. 1998;338:557-63. https://doi.org/10.1056/NEJM199802263380901
  7. Kassabian VS. Sexual function in patients treated for benign prostatic hyperplasia. Lancet. 2003; 361:60-2. https://doi.org/10.1016/S0140-6736(03)12164-2
  8. Fabian KM. Der Intraprostatische "Partielle Katheter" (Urologische spirale). Urologe A. 1980;19:236-8.
  9. Fabian KM. Der Intraprostatische "Partielle Katheter" (Urologische spirale) II. Urologe A. 1984;23:229-33
  10. Yorushalmi A, Fishelovitz Y, Singer D, Reiner L, Arielly J, Abramovie Y, et, al. Localized deep microwave hyperther mia in the treatment of poor operative risk patients with benign prostatic hyperplasia. Urology. 1985;133:873-6.
  11. 李惠義. 中醫男科講座. 北京: 中國醫藥科技出版社; 1992, p. 192-207.
  12. 杜鎬京. 東醫腎系學. 서울: 東洋醫學硏究院; 2003, p. 90, 705-8.
  13. 全國韓醫科大學鍼灸經穴學敎室. 鍼灸學(上, 下 ).서울: 集文堂; 1992, p. 1457.
  14. 심윤섭, 송호섭. 멜라틴이 세포자멸사 유발에 의해 DU-145 세포증식에 미치는 영향. 2009;26(3) :49-58.
  15. 이홍선, 송호섭. 봉독의 세포자멸사를 통한 LNCaP 세포증식 억제. 2008;25(2):59-74.
  16. Prieto JC, Carmena MJ. Receptors for vasoactive interstinal peptide on isolated epithelial cells of rat ventral prostate. Biochim Biophys Acta. 1983;763:408-13. https://doi.org/10.1016/0167-4889(83)90102-7
  17. Carmena MJ, Prieto JC. Cyclic AMP-stimulating effect of vasoactive intestinal peptide in isolated epithelial cells of rat ventral prostate. Biochim Biophys Acta. 1983;763:414-8. https://doi.org/10.1016/0167-4889(83)90103-9
  18. 大韓病理學會. 病理學(3판). 서울: 고문사; 1995, p. 877-80.
  19. 황종찬. 前立腺疾患의 豫防과 治療法. 서울: 태을출판사; 1998, p. 20-8, 95-107, 139-200
  20. 임영남. 前立腺疾患이렇게 治療한다. 서울: 한줄기; 1994, p. 11-3, 71-5.
  21. 김세철. 前立腺疾患의 모든 것. 서울: 삼신문화사; 1997, p. 1-6, 43-4, 51-62, 73-122.
  22. 이진선, 권기록, 최호영. HPLC를 이용한 봉약침의 주요 성분에 관한 연구. 대한침구학회지. 2000;17(4):125-6.
  23. Tewari A, Shinahare K, Narayan P. Transition zone volume and transition zone ratio: predictor of uroflow response to finasteride therapy in benign prostatic hyperplasia patient. Urology. 1995;45:258-65. https://doi.org/10.1016/0090-4295(95)80015-8
  24. 홍주의, 홍성준. 5-알파환원효소억제제(Finasteride) 투약 중단 후 보이는 전립선 크기, 전립선이행대 크기 및 PSA 변화 양상. 1999;40(11):1519-24.
  25. Barry M, Roehrborn C. Benign prostatic hyperplasia. Clin Evid. 2002;7:775-87.
  26. 박동수, 이용규, 정희성, 조택우, 이진하, 홍영권, 이영태. 하부요로증상을 보이는 전립선비대증에서 전립선 내 Botulinum Toxin Type A(BTA) 주입요법의 초기경험과 경요도적 전립선절제술과의 단기효과 비교.대한비뇨기과학회지.2005; 46(11):1173-79.
  27. 朱文蜂. 실용중의사전. 陜西: 陜西科學技術出版社; 1995, p. 402.
  28. Lee JH, Kwon YB, Han HJ, Mar WC, Lee hj, Yang IS, Beitz AJ, Kang SK. Bee venom pretreatment has both an antinociceptive and anti-inflammatory effect on carageenan-induced inflammation . J Vet Med Sci. 2001;63(3):251-9. https://doi.org/10.1292/jvms.63.251
  29. 권기록, 고형균, 봉약침요법의 면역반응에 관한 임상적 연구, 대한침구학회지. 2000;17(1):169-74.
  30. 박찬열, 남상수, 김창환, 이재동, 강성길, 이윤호, 안병철. 약침용 봉독액이 흑색세포에 미치는 항암효과에 대한 분자생물학적 연구, 대한침구학회지. 2000;17(2):169-86.
  31. Annila I, Saarinen JV, Nieminen MM, Moilanen E, Hahtola P, Harvima IT, Bee veom induces high hiastamine or high high orukotriene C4 release in skiotriesensitized beekeepers. J Investig Allergol Clin Immunol.. 2000;10(4):223-8.
  32. 약침학. 대한약침학회 학술위원회. 2008, p. 167-99.
  33. 박청, 박종일, 윤은진, 송경섭, 김종석, 김영래 등. 백서에서 고환적출 후 복측전립선의 Apoptosis기전. 대한내분비학회지. 2005;20(3):230-41.
  34. Smith CA, Williams GT, Kingstone R, Jenkinson EJ, Owen JJT: Antibodies to CD3/T-cell receptor complex induce cell death by apoptosis in immature T cells in thymic cultures. Nature. 1995;374:811-4. https://doi.org/10.1038/374811a0
  35. Kerr JFR, Searle J, Harmon BV, Bishop CJ: Apoptosis. In : Potten CS (ed), Perspectives on Mammalian cell Death, Oxford University Press. 1987;201:93-4.
  36. Chung WLK, McFadden DK: Sex steroid imprinting and prostate growth. Invest Urol. 1980;17:337-42.
  37. Sun H, Li TJ, Sun LN, Qju Y, Huang BB, Yi B, et al. Inhibitory effect of traditional Chinese medicine Zi-Shen Pill on benign prostatic hyperplasia in rats. J Ethnopharmacol. 2008; 115(2):203-8. https://doi.org/10.1016/j.jep.2007.09.026
  38. Shapiro E, Becich MJ, Hartanto V, Lepor H. The relative proportion of stromal and epithelial hyperplasia is related to the development of symptomatic benign prostatie hyperplasia. J Urol. 1992;147:1293-7.