The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
권호 표지

The Antiallergic and Antioxidative Effects of Chaenomeles Sinensis (CS) in RBL 2H3 Cells

목과(木瓜) 추출물이 항알레르기 및 항산화 작용에 미치는 영향

  • Lee, Seung-Yeon (Department of Pediatrics, College of Oriental Medicine, Dongeui University)
  • 이승연 (동의대학교 한의과대학 소아과학교실)
  • Received : 2010.07.23
  • Accepted : 2010.08.16
  • Published : 2010.08.31
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Abstract

Objectives The purpose of this study was to investigate the antiallergic and antioxidative effects of Chaenomeles Sinensis (CS). Methods CS pretreatments inhibited anti-DNP IgE in RBL-2H3 mast cells for an hour. we measured cell viability, $\beta$-hexosaminidase release, IL-4, TNF-$\alpha$ secretion, and IL-4, TNF-$\alpha$ mRNA expression CS pretreatments inhibited DNP-HSA($10\;{\mu}g/ml$) for ten minutes, we measured Dicholrodihydrofluorescein(DCF) and DPPH radical-scavenging activity in 0.5 mM 1,1-diphenyl-2-picrydrazyl(DPPH) radical solution, 0.1ml, 99% ethanol 0.8ml, and CS 0.1 ml mixed solution. Results 0, 1, 2, 3, 4, 5 mg/ml CS treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-$\alpha$ secretion, CS treatments also decreased IL-4, TNF-$\alpha$ mRNA expression in RBL-2H3 cells. CS treatments inhibited reactive oxygen species(ROS) and DPPH radical-scavenging activity. Conclusions These results suggest that CS may be useful for the prevention or treatment of allergic disease.

Keywords

References

  1. Adams LS, Seeram NP Aggarwal BB, Takada Y, Sand D, Heber D. Pomegranate juice, total pomegranate ellagitannins, and punicalagin supress inflammatory cell signaling in colon cancer cells. J.Agr.Food Chem. 2006;54:980-5. https://doi.org/10.1021/jf052005r
  2. Ahn DG, Illustrated book of korean medicinal herbs. Seoul:kyohak publishing Co. 2000;742.
  3. Jung KH, Kim MJ, Ha E, Uhm YK, Chung JH, Yim SV. Suppressive effect of Punica granatum on the production of tumor necrosis factor in BV2 microglial cells. Biol.Pharm.Bull. 2006;29:1258-61. https://doi.org/10.1248/bpb.29.1258
  4. Lansky EP, Newman RA. Punica granatun and its potential for prevention and treatment of inflammation and cancer. J.Ethnopharmacol. 2007;109:177-206. https://doi.org/10.1016/j.jep.2006.09.006
  5. Li Y, Qi Y, Huang TH, Yamahara J, Roufogalis BD. Pomegranate flower : A unigue traditional antidiabetic medicine with dual PPAR-alpha/gamma activator properities. Diabetes Obes. Metab. 2008;10:10-7.
  6. Marletta MA, Tayeh MA, Hevel JH. Unraveling the biological significance of nitric oxide. Bidfactors. 1009;2:219-25.
  7. Wolfreys K, Oliviera D. Alterations in intracellular reactive oxygen species generation and redox potential modulate mast cell function. Eur. J. Immunol. 1997:27;297-306. https://doi.org/10.1002/eji.1830270143
  8. Gushchin I, Petyaev I, Tsinkalovsky O. Kinetics of oxygen metabolism indices in the course of histamine secretion from rat mast cells. Agents Actions, 1009:30;85-8. https://doi.org/10.1007/BF01969005
  9. Cobb MH, Goldsmith EJ. Dimerization in MAP-kinase signaling. Trends Biochem. Sci. 200:25;7-9. https://doi.org/10.1016/S0968-0004(99)01508-X
  10. 이상인, 안덕균, 신민교, 이영종. 한약임상응용. 서울:성보사. 1986:182-3.
  11. Lee C B. Forest Economics-mokchogangmok, korean plant map. Hyangmunsa. 1982:133.
  12. Itoh T, Umekawa H, Furuichi Y. Potential ability of hot water adzuki (Vigna angularis) extracts to inhibit the adhesion, invasion, and metastasis of murine B16 melanoma cells. Biosci Biotechnol Biochem. 2005; 69(3):448-54. https://doi.org/10.1271/bbb.69.448
  13. 難波恒雄. 原色和漢藥圖鑑. 保育社. 大阪. 1980:195.
  14. 赤松金芳. 新訂 和漢藥. 東京:醫齒藥出版社. 1970: 355.
  15. 전국한의과대학 공동교재 편찬위원회. 본초학. 서울:영림사. 1007:315-6.
  16. 김창민 등. 중약대사전. 서울:정담. 1998:1688-94.
  17. 이상인. 본초학. 서울:의약사. 1975:179-180
  18. 허준. 동의보감. 서울:법인문화사. 1999:1888-9.
  19. 김덕곤 외. 동의 소아과학. 서울:정담. 2002:29.
  20. Kim YS, Lee SW, Lee KR Cho SY, Lee JH. Studies on the tasty constituents in verious foodstuffs. Korean J. Food Sci Technol 3. 1971:163-7.
  21. Chung TY, Cho DS, Song JC. Nonvoletile/voletic flavor conponents in chinese auinine. Korean J. Food Sci Technol. 20:293-302.
  22. Lee DH, Kim JH,Kim NM, Choi JS, LJS. Physiological functionality of chinese quinine wine and liquors. Korean J Biotechnol Bioeng. 2002;17:266-70.
  23. 이태진 등. 목과 호장근 및 유향 추출물이 Raw 264.7 cell에서 LPS로 유도된 nitric oxide 생성에 미치는 영향. 동의생리병리학회지. 2006;20(3);603-8.
  24. 유지현, 한신희, 길기정. 모과 추출물의 항응혈 활성. 대한본초학회지. 2009:24(2);7-11.
  25. 이유미, 신덕형, 이재준, 이명렬, 모과 에탄올 추출물의 항산화 효과. Koeran J.Food Preserv. 2007:14(2);177-82.
  26. 이유미, 이재누, 신덕형, 이명렬. 에탄올에 의해 유발된 간독성에 대한 모과 추출물의 보호 효과. J Korean Soc Food SCI Nutr. 2006:35(10);1336-42. https://doi.org/10.3746/jkfn.2006.35.10.1336
  27. Metcalfe DD, Kaliner M, Donlon MA. The mast cell. Crit. Rev. Immunol. 1981:3;23-74.
  28. Ahn K. Role of mast cells in allergic inflammation and innate immunity. Korean J. Pediatr. 2004:47;1137-41.
  29. Marshall JS. Mast cell responses to pathogens. Nat. Rev. Immunol. 2004:4;787-99. https://doi.org/10.1038/nri1460
  30. Nadler MJ, Matthews SA, Turner H, Kinet JP. Signal transduction by the high-affinity immunoglobulin E receptor $Fc{\varepsilon}RI$: Coupling form to function. Adv. Immunol. 2000:76;325-55.
  31. Mekori YA, Metcalfe DD. Mast cells in innate immunity. Immunol. Rev. 2000:173;131-40. https://doi.org/10.1034/j.1600-065X.2000.917305.x
  32. Petersen LJ, Mosbech H, Skov PS. Allergen-induced histamine release in intact human skin in vivo asessed by skin microdialysis technique: Characterization of factors influencing histamine releasability. J. Allergy Clin. Immun. 1996:97;672-9. https://doi.org/10.1016/S0091-6749(96)70313-5
  33. Marquardt DL, Wasserman SI. Modulation of rat serosal mast cell biochemistry by in vivo dexamethasone administration. J Immunol. 1983;131:934-9.
  34. Mastuda H, Morikawa T, Ueda K, Managi H, Yoshikawa M. Structural requirements of flavonoids for inhibition of antigen-Induced degranulation, TNF-alpha and IL-4 production from RBL-2H3 cells. Bioorg Med Chem. 2002;10:3123-8. https://doi.org/10.1016/S0968-0896(02)00227-4
  35. Iikura M, Yamaguchi M, Hirai K, Miyamasu M, Yamada H, Nakajima T, Fujisawa T, Ra C, Morita Y, Yamamoto K. Regulation of surface FcepsilonRI expression on human eosinophils by IL-4 and IgE. Int Arch Allergy Immunol. 2001;124:470-7. https://doi.org/10.1159/000053782
  36. 이중달. 그림으로 설명한 병리학. 서울: 고려의학. 1991:29.
  37. De Martinis M, Franceschi C, Monti d, Ginaldi L. Inflamma-ageing and lifelong antigenetic load as major determinats of aging rate and longevity. FEBS Latt. 2005;579:2035-9. https://doi.org/10.1016/j.febslet.2005.02.055
  38. Ginaldi L, De Martinis M, Monti D, Franceschi C. Chronic antigenic load and apoptosis in immunosenescence. trends Immunol.2005;26:79-84. https://doi.org/10.1016/j.it.2004.11.005
  39. Virag LE, Szabo Gergely and C Szabo. Peroxynitrite induced cytotoxicity: mechanism and opportunities forintervention. Toxicol Lett. 2003;140-1:113-24. https://doi.org/10.1016/S0378-4274(02)00508-8
  40. Balavoine GGA and YV Geletii. Peroxynitrite scavenging by different antioxidants part I :convenient assay, Nitric Oxide:Biology and Chemistry 1999;3:40-54. https://doi.org/10.1006/niox.1999.0206
  41. Davis KJ. Oxidative stress:the paradox of aerobic life. Biochem. Soc Symp. 1999:21-36.