YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

The Simultaneous Analysis of Sennoside A and Rhaponticin in Rhei Rhizoma using Liquid Chromatography-Diode Array Detecter

HPLC/DAD를 이용한 대황의 Sennoside A와 Rhaponticin 동시분석

  • Kim, Ouk-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kwak, Jae-Eun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jung, Sam-Ju (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Dong-Gyu (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han, Eun-Jung (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han, Chang-Ho (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Bok-Soon (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Choi, Byung-Hyun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Min-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • Received : 2009.09.28
  • Accepted : 2009.11.18
  • Published : 2010.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of this study was to analyze sennoside A and rhaponticin simultaneously according to sennoside A of Rhei Rhizoma determination in Korean Pharmacopoeia. $NaHCO_3$ solution in KP was compared with methanol which usually used as solution to extract rhaponticin in Rhei Rhizoma. The method was validated through the guidelines of linearity, LOD, LOQ, specificity and accuracy. Two solution weren't different about validation parameter and passed. So this method were applied to the determination of 6 commercial Rhei Rhizoma samples and 2 samples were suitable for the legal standards.

Keywords

References

  1. 김호철 : 한약약리학, 집문당, 경기도, p. 174 (2001).
  2. Ko, S. K., Lee, S. M. and Whang, W. K. : Effects of Rheum plants on blood platelet aggregation. Yakhak Hoeji 43, 233 (1999).
  3. Kang, J. S., Park, K. J., Wu, E., Lee, E. S., Hwang, G. S., Lee, H. S. and Kim, Y. H. : Comparative study of the Rhei Rhizoma by pattern analysis. Kor. J. Pharmacogn. 39, 179 (2008).
  4. Kim, J. Y., Choi, G. Y., Kim, H. J. and Ju, Y. S. : The external and internal morphological standards of original plants and herbal states in two kinds of Rhei Rhizoma. Korean Journal of Oriental Medicine 12, 39 (2006).
  5. 생약학교재편찬위원회 : 생약학. 동명사, 서울, p. 225 (2008).
  6. Ko, S. K., Shin, C. G., Lee, H. S., Han, S. T., Yang, B. W., Im, B. O. and Chung, S. H. : Effect of stilbene derivatives from Rheum undulatum on carrageenan-induced acute edema in rats. Kor. J. Pharmacogn. 35, 171 (2004).
  7. Matsuda, H., Tomohiro, N., Hiraba, K., Harima, S., Ko, S. K., Matsuo, K., Yoshikawa, M. and Kubo, M. : Study on anti-Oketsu activity of rhubarb. Anti-allergic effects of stilbene components from Rhei undulati Rhizoma. (dried Rhizome of Rheum undulatum cultivated in Korea) Biol. Pharm. Bull. 24, 264 (2001). https://doi.org/10.1248/bpb.24.264
  8. Lee, Y. M., Lee, S. W., Cho, J. H. and Kim, J. S. : Quality evaluation of Rhei Rhizoma which are sold in Korea, China and Japan. Korean Journal of Oriental Medicine 10, 101 (2004).
  9. Kim, S. J., Han, S. H. and Lee, Y. J. : A study on a morphological identification of kinds of Rhei Rhizoma. Kor. J. Herbology 21, 171 (2006).
  10. Kim, M. W., Chang, S. Y., Lee, J. H., Ko, S. K. and Yook, C. S. : Studies on determination of sennoside A and sennoside B in Rheum spp.. Bull. K. H. Pharma. Sci. 28, 115 (2000).
  11. Kim, S. Y. : Study on the major component of Rhei Rhizoma and Rhei Unduratai Rhizoma. Graduate school of Kyung Hee University, Seoul, Feb (2006).
  12. Choi, S. K. : Quantitative analysis of sennoside A and freeantraquinones in various Rhei Rhizoma from current market. Graduate school of Kyung Hee University, Seoul, July (2007).
  13. Ye, M., Han, J., Chen, H., Zheng, J. and Guo, D. : Analysis of phenolic compounds in rhubarbs using liquid chromatography coupled with electrospray ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 18, 82 (2007). https://doi.org/10.1016/j.jasms.2006.08.009
  14. Schnelle, F. J. and Schratz, E. : Differences in occurrence of anthraquinone aglycons and rhaponticin in Rheum species. Planta Medica 14, 194 (1966). https://doi.org/10.1055/s-0028-1100044
  15. Lin, C. C., Wu, C., Lin, C. L. and Sheu, S. J. : Determination of 19 rhubarb constituents by high-performance liquid chromatography-ultraviolet-mass spectrometry. J. Sep. Sci. 29, 2584 (2006). https://doi.org/10.1002/jssc.200500307
  16. Komatsu, K., Nagayama, Y., Tanaka, K., Ling, Y., Cai, S. Q., Omote, T. and Meselhy, M. R. : Comparative study of chemical constituents of rhubarb from different origins. Chem. Pharm. Bull. 54, 1491 (2006). https://doi.org/10.1248/cpb.54.1491
  17. Komatsu, K., Nagayama, Y., Tanaka, K., Ling, Y., Cai, S. Q., Omote, T. and Meselhy, M. R. : Development of a high performance liquid chromatographic method for systematic quantitatie analysis of chemical constituents in rhubarb. Chem. Pharm. Bull. 54, 941 (2006). https://doi.org/10.1248/cpb.54.941
  18. Komatsu, K., Yang, D. Y., Fushimi, H. and Cai, S. Q. : Authentication of Rhei Rhizoma. J. Trad. Med. 22(Suppl.1), 70 (2005).
  19. Ko, S. K., Han, S. T., Yang, B. W., Shin, C. G., Hahm, Y. T., Cho, S. H. and Whang, W. K. : Quantitative analysis of anthraquinone and stilbene derivatives in various rhubarbs. Kor. J. Phramacogn. 36, 257 (2005).
  20. 식품의약품안전청 : 대한약전. 9개정, 신일북스, 서울 (2008).
  21. Yoo, J. L., Jang, D. S. and Kim, J. S. : Quality evaluation of herbal prescription, Oc Chun San, employing simultaneous determination of the marker compounds by HPLC. Korean J. Oriental Medicine 11, 167 (2005).
  22. Feng, C., Cai, Y. L. and Ruan, J. L. : Simultaneous determination of 10 active components in traditional chinese medicine "YIGONG" capsule by RP-HPLC-DAD. J. Pharm. Biomed. Anal. 47, 442 (2008). https://doi.org/10.1016/j.jpba.2008.01.019
  23. Xie, Y., Jiang, Z. H., Zhou, H., Cai, X., Wong, Y. F., Liu, Z. Q., Bia, Z. X., Xu, H. X. and Liu, L. : Combinative method using HPLC quantitative and qualitative analyses for quality consistency assessment of a herbal medicinal preparation. J. Pharm. Biomed. Anal. 43, 204 (2007). https://doi.org/10.1016/j.jpba.2006.07.008
  24. O'Neil, M. J., Smith, A., Heckelman, P. E., Obenchain Jr., J. R., Gallipeau, J. A. R. and D'arecca, M. A. : The merck index. Merck & Co., inc., 13th, p. 8526 (2001).
  25. O'Neil, M. J., Smith, A., Heckelman, P. E., Obenchain Jr., J. R., Gallipeau, J. A. R. and D'arecca, M. A. : The merck index. Merck & Co., inc., 13th, p. 8259 (2001).
  26. Koyama, J., Morita, I. and Kobayashi, N. : Simultaneous determination of anthraquinones in rhubarb by high-performance liquid chromatography and capillary electrophoresis. J. Chromatogr. A 1145, 183 (2007).
  27. 식품의약품안전청 : 의약품 등 분석법의 밸리데이션에 대한 가이드라인 (2004)
  28. ICH harmonized tripartite guideline. Validation of analytical procedures. Methodology Q2B, ICH steering committee (1996)
  29. Xu, L., Han, X., Qi, Y., Xu, Y., Yin, L., Peng, J., Liu, K. and Sun, C. : Multiple compounds determination and fingerprint analysis of lidanpaishi tablet and keli by high-performance liquid chromatography. Anal. Chim. Acta 633, 136 (2009). https://doi.org/10.1016/j.aca.2008.11.043
  30. Wang, J., Li, H., Jin, C., Qu, Y. and Xiao, W. : Development and validation of a UPLC method for quality control of rhubarbbased medicine: Fast simultaneous determination of five antraquinone derivatives. J. Pharm. Biomed. Anal. 47, 765 (2008). https://doi.org/10.1016/j.jpba.2008.03.011
  31. Lee, G. W., Kim, A. R. and Cho, J. S. : HPLC analysis of organic acids, phenol and benzopyrene in wood vineger. Yakhak Hoeji 52, 12 (2008).
  32. Shang, X. and Yuan, Z. : Determination of six components in rhubarb by cyclodextrin-modified micellar electrokinetic chromatography using a mixed micellar system of sodium cholate and sodium taurocholate. Anal. Chim. Acta 456, 183 (2002). https://doi.org/10.1016/S0003-2670(02)00044-2
  33. Kashiwada, Y., Nonaka, G. and Nishioka, I. : Studies on rhubarb (Rhei Rhizoma). XV. Simultaneous determination of phenolic constituents by high-performance liquid chromatography. Chem. Pharm. Bull. 37, 999 (1989). https://doi.org/10.1248/cpb.37.999