약학회지 (YAKHAK HOEJI)

  • 제54권3호
  • /
  • Pages.151-156
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  • 2010
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  • 0377-9556(pISSN)
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  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

갈근탕 추출물의 카드뮴 독성에 대한 세포 보호효과

Cytoprotective Effect of Galgeuntang Extract on Cadmium-Induced Cytotoxicity

  • 박소윤 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 백종민 (서울대학교 비알엔사이언스 연구개발부) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Park, So-Yun (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Baek, Jong-Min (R&D Center, BRNScience Co. LTD., Seoul National University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 투고 : 2009.09.24
  • 심사 : 2010.03.20
  • 발행 : 2010.06.30
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초록

Cytotoxicity of cadmium on NIH 3T3 fibroblasts was utilized in order to discover antitoxic compound in Galgeuntang extract in this study. Treatment groups were chosen as follows; control (medium only), $MTT_{50}$ group and five experimental groups. MTT {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide} method was performed to evaluate the cytotoxicity of cell organelles and $IC_{50}$ was also measured. Accordingly we have examined the detoxification effects of Galgeuntang extract on cadmium-treated NIH 3T3 fibroblasts to observe morphological changes by the light microscopy. Galgeuntang extract showed cytoprotective effects on cadmium-induced cytotoxicity. Furthermore, Galgeuntang showed a dose-dependency in detoxication. The phenolic content of Galgeuntang ethanol extract was higher than that of water content. These results suggest that Galgeuntang extract may be used as a cytoprotective agent against cadmium (II)-mediated cytotoxicity.

키워드

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