Production of Fermented Tea with Rhodotorula Yeast and Comparison of its Antioxidant Effects to those of Unfermented Tea

적색효모를 이용한 발효차의 제조 및 발효차와 비발효차의 항산화능 비교

  • Kang, Ok-Ju (Department of Food and Nutritional Science, Kyungnam University)
  • 강옥주 (경남대학교 식품영양학과)
  • Received : 2010.06.25
  • Accepted : 2010.07.22
  • Published : 2010.08.31

Abstract

This study was conducted to investigate the antioxidant effect and properties of unfermented tea and fermented tea made with Rhodotorula yeast. The levels of crude fat and crude protein in the fermented tea were higher than those in the unfermented tea. The water-soluble phenol levels of unfermented tea and fermented tea were 912.5 and 2,445.24 ppm, respectively. The total amino acid content of fermented tea was greater than that of unfermented tea;,- the amino acid concentrations of alanine, valine, leucine, and lysine were 25.58, 24.38, 27.96, and 14.14 ${\mu}g/mL$, respectively. The DPPH radical scavenging activities of the unfermented and fermented teas were 32.14 and 41.57%, respectively; this is in contrast to 29.73% for L-ascorbic acid(150 ppm). The reducing power activity of fermented tea was 41.57%, and that of unfermented tea was 32.14%.

Keywords

References

  1. 김종태. 1996. 차의 과학과 문화. 도서출판 보림사. 서울. pp 8-153
  2. A.O.A.C. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists. Washington D.C. pp. 69-74
  3. Bae KH, Ryu HY, Kwun IS, Kwon CS, Sohn HY. 2007. Optimization of Thickness and Maturation Period of Andong-Soju Nuruk for Fermentation of Andong-Soju. Korean J Microbiol Biotechnol 35(3):231-237
  4. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 4617:1198-1200
  5. Choi HJ, Lee WS, Hwang SJ, Lee IJ, Shin DH, Kim HY, Kim KU. 2000. Change in chemical compositions of green tea under the different extraction conditions., Korean J Life Sci 10(2):202-209
  6. Choi OJ, Choi KH. 2002. The physicochemical properties of Korean wild tea(Green tea, Semi-fermented tea, and Black tea) according to degree of fermentation. J. Korean Soc Food Nutr 32(3):356-362 https://doi.org/10.3746/jkfn.2003.32.3.356
  7. Chung YH, Shin MK. 2005. A Study on the physicochemical properties of korean Teas according to degree of fermentation. Korean J. Food & Nutr 18(1):94-101
  8. Kang OJ. 2008. Isolation and identification of yeast strain from fermented tea. Korean J Food Cookey Sci 24(1):11-15
  9. Kim HS, Yu TS. 2000. Volatile flavor components of traditional Korean nuruk produced by nuruk fungi. Korean J Appl Microbiol Biotechnol 28(5):303-308
  10. Kim JH, Lee DH, Lee SH, Choi SY, Lee JS. 2004. Effect of Ganoderma lucidum on the quality and functionality of Korean traditional rice wine yakju. J Biosci Bioeng 97(1): 24-28
  11. Kim MH, Kim MC, Park JS, Kim JW, Lee JO. 2001. The antioxidative effects of the water-soluble extracts of plants used as tea materials. Korean J Food Sci Tech 33(1): 12-18
  12. KSFSN(Korean Society of Food Science Nutrition). 2000. Handbook of Experiments in Food Science and Nutrition. Hyoil Co. Seoul, Korea. pp. 260-261, 321
  13. Lee JW, Do JH, Shin KM. 1999. Antioxidant activity of the water soluble browning reaction products isolated from Korean Red Ginseng. 1. DPPH radical and hydrogen peroxide scavenging. J Gonseng Res 23(3):176-181
  14. Lee DI, Heo TR, Kim DH, 1975. Comparison of the antioxidant effects of ethyl alcohol extracts of Maillard-type and a caramelization type browning reaction mixtures. Korean J Food Sci Tech 7(1):43-50
  15. Lee JW, Park CK, Do JH. 2005. Antioxidative activity of the water soluble browning reaction products from Korean Red Ginseng. J Ginseng Res 29(1):44-48 https://doi.org/10.5142/JGR.2005.29.1.044
  16. Markham KR. 1982. Techniques of flavonoids identification. Academic press. London, UK. pp.37
  17. Oyaizu M. 1988. Antioxidative activities of browning products of glucosamine fractionated by organic solvent and thin-layer chromatography. Nippon Shokuhin Kogyo Gakkaishi, 35:771-775 https://doi.org/10.3136/nskkk1962.35.11_771
  18. Park CO. 1996. Antioxidant activity of boiling water extracts obtained from green tea. Doctorate thesis. Pusan National University. pp 26-30
  19. Ramarathnam N, Osawa T, Ochi H, Kawakishi S. 1995. The contribution of plant food antioxidants to human health. Trends Food Sci Tech 6(3):75-82 https://doi.org/10.1016/S0924-2244(00)88967-0
  20. Seob UH, Choe HJ, Han HS, Park JH, Son JH, An BJ, Son GM, Choe C. 2003. Isolation of polyphenol from green tea by HPLC and its physiological activities. Korean J Food Sci Tech 35(6):1199-1203
  21. Somogyi M. 1952. Notes in sugar determination. J Biol Chem 195:19-23
  22. Tsuyoshi NR, Fudou R, Yamanak S, Kozaki M, Tamang N, Thapa S, Tamang P. 2005. Identification of yeast strains isolated from marcha in sikkim, a microbial starter for amylolytic fermentation. Int J Food Microbiol 99(2):135-146 https://doi.org/10.1016/j.ijfoodmicro.2004.08.011