Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Mixed Medicinal Herbs on Adipogenesis and Apoptosis in 3T3-L1 Adipocytes

배합된 한약재의 추출물이 3T3-L1 지방세포의 지방생성과 세포사멸에 미치는 영향

  • Hwang, Su-Jung (Department of Herbal Pharmacology, College of Oriental Medicine, & R&D Team for the New Drug of Oriental Medicine (BK 21), Daegu Haany University) ;
  • Shin, In-Soon (Department of Herbal Pharmacology, College of Oriental Medicine, & R&D Team for the New Drug of Oriental Medicine (BK 21), Daegu Haany University) ;
  • Kim, Mi-Ryeo (Department of Herbal Pharmacology, College of Oriental Medicine, & R&D Team for the New Drug of Oriental Medicine (BK 21), Daegu Haany University)
  • 황수정 (대구한의대학교 한의과대학 본초약리학교실 & BK21 한방신약개발연구팀) ;
  • 신인순 (대구한의대학교 한의과대학 본초약리학교실 & BK21 한방신약개발연구팀) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실 & BK21 한방신약개발연구팀)
  • Received : 2010.07.19
  • Accepted : 2010.08.16
  • Published : 2010.08.25
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Abstract

Astragali Radix (AR) has been used in traditional medicinal herb, which is known to tonify the qi and blood. It has been asserted to be a tonic that can improve the function of the lungs, adrenal glands and the gastrointestinal tract, increase metabolism, promote healing and reduce fatigue. Also, Plantago asiatica (PA) has the anti-inflammatory, antiseptic, antitussive, cardiac, diuretic, expectorant, haemostatic effects, though it is considered to be less powerful than the seed. Recent research is showing that it is promising in lowering cholesterol and controlling diabetes. Obesity is characterized at the cellular level by an increase in the number and size of adipocytes differentiated from fibroblastic preadipocytes in adipose tissue. In the present study, we investigated the effects of 3 to 7 extracts of mixed medicinal herbs (water and ethanol extract of Astragali Radix; ARW and ARE, water and ethanol extract of Plantago asitica; PAW and PAE, and those mixed extracts; ARW+PAE, ARW+PAW and ARE+PAE) on adipogenesis and apoptosis in 3T3-L1 adipocytes by MTT assay, Oil-Red-O staining and TUNEL assay. And then, we determined total contents of phenolic compounds and flavonoid compounds in mixed herbal extracts. These result showed that herbal extracts (ARW, PAE and ARW+PAE) affected on adipogenesis and apoptosis in 3T3-L1 adipocytes among 3 to 7 extracts tested. Especially herbal mixed extracts (ARW+PAE) had the highest inhibition on differentiation of preadipocytes and lipoid accumulation of maturing adipocytes. And it did not affect cell viability of mature adipocytes, but herbal mixed extracts (ARW+PAE) increased apoptosis in mature adipocytes, as proved by highest concentration $200\;{\mu}g/m{\ell}$ using TUNEL assay. In addition, contents of total phenoilc compounds and flavonoids showed the highest level in ARE+PAE and PAE than ARW. These results suggest that this main target for adipocyte functions could be effective in improving the metabolic syndrome including obesity.

Keywords

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