Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effects of Water Extract of Glycyrrhiza uralensis on $\beta$-Hexosaminidase Release and Expression of the Cytokines of RBL-2H3 Mast Cells

감초 추출물이 RBL-2H3 비만세포에서 $\beta$-hexosaminidase 분비 및 Th2 cytokine mRNA 발현에 미치는 효과

  • Kim, Jeong-Mi (Department of Bio and Health Technology, Kangwon National University) ;
  • Kim, Dae-Jung (Department of Bio and Health Technology, Kangwon National University) ;
  • Kim, Tae-Hyeuk (Department of Bio and Health Technology, Kangwon National University) ;
  • Baek, Jong-Mi (Department of Bio and Health Technology, Kangwon National University) ;
  • Kim, Hyun-Sook (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Choe, Myeon (Department of Bio and Health Technology, Kangwon National University)
  • 김정미 (강원대학교 생명건강공학과) ;
  • 김대중 (강원대학교 생명건강공학과) ;
  • 김태혁 (강원대학교 생명건강공학과) ;
  • 백종미 (강원대학교 생명건강공학과) ;
  • 김현숙 (강원대학교 웰빙특산물산업화지역혁신센터) ;
  • 최면 (강원대학교 생명건강공학과)
  • Received : 2010.06.03
  • Accepted : 2010.08.05
  • Published : 2010.08.30
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Abstract

The present study was conducted to investigate the anti-allergic reaction with Glycyrrhiza uralensis. We examined cell viability, $\beta$-hexosaminidase release, IL-4 and IL-13 mRNA expression from RBL-2H3 cell after pre-treatment with 0, 100, 250, 500, 1000${\mu}g/m{\ell}$ of Glycyrrhiza uralensis water extracts. Effects of Glycyrrhiza uralensis on the degranulation and pro-inflammatory cytokines (IL-4 and IL-13) expression were evaluated with $\beta$-hexosaminidase assay, and RT-PCR analysis. We observed that Glycyrrhiza uralensis concentrations from 100${\mu}g/m{\ell}$ to 1000${\mu}g/m{\ell}$ had no effect on cell survival. The release of $\beta$-hexosaminidase decreased significantly with all concentrations of Glycyrrhiza uralensis extracts. The expression of the IL-4 and IL-13 mRNA were decreased by Glycyrrhiza uralensis in dose-dependent manner. These results that Glycyrrhiza uralensis has an anti-histamin effects and controls IL-4, IL-13 secretion on allergic reaction.

Keywords

References

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