Effect of 11-ketotestosterone (11-KT) on Gonadal Sex Reversal and Spermatogenesis of Honeycomb Grouper Epinephelus merra

  • Lee, Chi-Hoon (Marine & Environmental Research Institute, Jeju National University) ;
  • Hur, Sang-Woo (Marine & Environmental Research Institute, Jeju National University) ;
  • Song, Young-Bo (Marine & Environmental Research Institute, Jeju National University) ;
  • Takano, Kazunori (Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus) ;
  • Takemura, Akihiro (Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus) ;
  • Lee, Young-Don (Marine & Environmental Research Institute, Jeju National University)
  • 투고 : 2009.01.08
  • 심사 : 2010.03.01
  • 발행 : 2010.03.31

초록

We investigated the androgenic effects of 11-ketotestosterone (11-KT) on gonadal sex reversal and spermatogenesis in honeycomb grouper Epinephelus merra by method of gonadal biopsy. 11-KT was injected intramuscularly at a concentration of 1 and $10{\mu}g$ body weight. The proportion of cross sectional area of the gonad occupied by each germ cell type was measured and compared pre- and post-injection group. During the sex change phase, the distribution ratio of oocytes was decreased in all fish of 11-KT treatment group while the distribution ratio of spermatocytes was increased than pre-injection group. In male phase, all fish of 11-KT treatment group shown the increased distribution ratio of spermatocytes, but the distribution ratio of spermatozoa was decreased than pre-injection group. The present results suggest that 11-KT can stimulate degeneration of oocytes, proliferation of spermatocytes and spermiation in honeycomb grouper.

키워드

참고문헌

  1. Alam MA, Bhandari RK, Kobayashi Y, Nakamura S, Soyano K, Nakamura M (2006) Changes in androgenproducing cell size and circulating 11-ketotestosterone level during female-male sex change of honeycomb grouper Epinephelus merra. Mol Reprod Dev 73:206-214. https://doi.org/10.1002/mrd.20393
  2. Bhandari RK, Komuro H, Nakamura S, Higa M, Nakamura M (2003) Gonadal restructuring and correlative steroid hormone profiles during natural sex change protogynous honeycomb grouper (Epinephelus merra). Zool Sci 20: 1399-1404. https://doi.org/10.2108/zsj.20.1399
  3. Borg B (1994) Androgens in teleost fishes. Comp Biochem Physiol 109C:219-245.
  4. Cardwell JR, Liley NR (1991) Hormonal control of sex and color change in the spotlight parrotfish, Sparisoma viride. Gen Comp Endocrinol 81:7-20. https://doi.org/10.1016/0016-6480(91)90120-U
  5. Fostier A, Jalabert B, Billard R, Brenton B, Zohar Y (1983) The gonadal steroids. In: Hoar WS, Randall DJ, Donaldson EM (eds.), Fish Physiology, vol. IXA. Academic Press, New York, pp 277-372.
  6. Fostier A, Le Gac F, Loir M (1988) Steroids in male reproduction. In: proc. Third International Symposium on Reproductive Physiology of Fish, University of St-Johns, Canada, pp 239-245.
  7. Grober MS, Jackson IMD, Bass AH (1991) Gonadal steroid affects LHRH preoptic cell number in a sex/role changing fish. J Neurobiol 22:734-741. https://doi.org/10.1002/neu.480220708
  8. Kobayashi Y, Lee YD, Takemura A, Takano K, Soyano K (2000) Histological observation of the sex change in honeycomb grouper, Epinephelus merra. In: Proc. 6th International Symposium on the Reproductive Physiology of Fish, Institute for Marine Research and University of Bergen, Norway, pp269.
  9. Kokokiris L, Fostier A, Athanassopoulou F, Petridis D, Kentouri M (2006) Gonadal changes and blood sex steroids levels during natural sex inversion in the protogynous Mediterranean red porgy Pagrus pagrus (Teleostei: Sparidae). Gen Comp Endocrinol 149:42-48. https://doi.org/10.1016/j.ygcen.2006.05.002
  10. Kroon FJ, Liley NR (2000) The role of steroid hormones in protogynous sex change in the blackeye goby, Coryphopterus nicholsii (TeleosteiL Gobiidae). Gen Comp Endocrinol 118:273-283. https://doi.org/10.1006/gcen.2000.7459
  11. Miura T, Miura C (2001) Japanese eel: a model for analysis of spermatogenesis. Zool Sci 18:1055-1063. https://doi.org/10.2108/zsj.18.1055
  12. Miura T, Miura C (2003) Molecular control mechanisms of fish spermatogenesis. Fish Physiol Biochem 28: 181-186. https://doi.org/10.1023/B:FISH.0000030522.71779.47
  13. Miura T, Yamauchi K, Nagahama Y, Takahashi H (1991) Induction of spermatogenesis in male Japanese eel, Anguilla japonica, by a single injection of human chorionic gonadotropin. Zool Sci 8:63-73.
  14. Mohamed AA, Miura T, Miura C, Yamauchi K (2001) Involvement of sex steroid hormones in the early stages of spermatogenesis in Japanese Huchen (Hucho perryi). Biol Reprod 65:1057-1066. https://doi.org/10.1095/biolreprod65.4.1057
  15. Nakamura M (1981) Effects of 11-ketotestosterone on gonadal sex differentiation in Tilapia mossambica. Bulletin Japanese Society of Scientific Fisheries 47(10): 1323-1327. https://doi.org/10.2331/suisan.47.1323
  16. Nakamura M, Hourigan TF, Yamaguchi K, Nagahama Y, Grau GE (1989) Histological and ultrastructural evidence for the role of gonadal steroids hormones in sex change in the protogynous wrasse Thalassoma duperrey. Env Biol Fish 24:117-136. https://doi.org/10.1007/BF00001282
  17. Ozon R (1972) Androgens in fishes, amphibians, reptiles and birds. In: Idler DR(ed.) Steroids in Non Mammalian Vertebrates. Academic Press, New York, pp 329-389.
  18. Schulz RW, Miura T (2002) Spermatogenesis and its endocrine regulation. Fish Physiol Biochem 26:43-56. https://doi.org/10.1023/A:1023303427191
  19. Takahashi H (1975) Masculinization of the gonad of juvenile guppy, Poecilia reticulate, induced by 11-ketotestosterone. Bull Fac Fish Hokkaido Univ 26:223-234.
  20. Tanaka H, Tsuchihashi Y, Kuromiya Y (2000) Induction of sex reversal in the sevenband grouper, Epinephelus septemfasciatus. In: Proc. 6th International Symposium on the Reproductive Physiology of Fish, Institute for Marine Research and University of Bergen, Norway, pp423.
  21. Yeh SL, Kuo CM, Ting YY, Chang CF (2003) Androgens stimulate sex change in protogynous grouper, Epinephelus coioides: spawning performance in sex-changed males. Comp Biochem Physiol Part C 135:375-382.