Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Endoreduplication in Phalaenopsis is affected by light quality from light-emitting diodes during somatic embryogenesis

  • Park, So-Young (Forest Biotechnology Division, Korea Forest Research Institute) ;
  • Yeung, Edward C. (Department of Biological Sciences, University of Calgary) ;
  • Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
  • Received : 2010.07.04
  • Accepted : 2010.08.09
  • Published : 2010.12.30
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Abstract

Endoreduplication is a developmental process that is unique to plants and occurs in all plants. The present study aimed to assess endoreduplication in various explant tissues and regenerated somatic embryos of Doritaenopsis. We further investigated the effects of light quality on endoreduplication and somatic embryo proliferation. To this end, we studied endoreduplication in leaves and root tips from regenerated plantlets and somatic embryos and in developing somatic embryos under 4 types of lighting conditions: red light, red + far-red light, red + blue light, and white light. We found that the degree of endoreduplication varied in different explants, and that the choice of explants used also influenced the ploidy levels of the newly regenerated somatic embryos. The DNA content of the leaf (2C-8C) was less than that of the root tip (2C-16C) and somatic embryo (2C-64C). In terms of light quality, the combination of red and far-red light produced the highest number of somatic embryos, while maintaining a low degree of endoreduplication. The data obtained indicate that this light combination stimulates somatic embryogenesis in Doritaenopsis and may exert some control on endoreduplication during cell division. These findings can be applied to achieve a reduction in somaclonal variations for the purpose of mass proliferation and genetic improvement.

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References

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