Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

In vitro regeneration of Lycaste aromatica (Graham ex Hook) Lindl. (Orchidaceae) from pseudobulb sections

  • Mata-Rosas, Martin (Red Manejo Biotecnologico de Recursos, Instituto de Ecologia) ;
  • Baltazar-Garcia, Rosario J. (Red Manejo Biotecnologico de Recursos, Instituto de Ecologia) ;
  • Moon, Pamela (Tropical Research and Education Center, University of Florida) ;
  • Hietz, Peter (Institute of Botany, Department of Integrative Biology, University of Natural Resources and Applied Life Sciences (Boku)) ;
  • Luna-Monterrojo, Victor E. (Red Manejo Biotecnologico de Recursos, Instituto de Ecologia)
  • Received : 2009.11.11
  • Accepted : 2010.02.03
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A protocol for in vitro propagation from pseudobulb sections of Lycaste armomatica (Graham ex Hook) Lindl., an ornamental and fragrant orchid, was developed. The effect of four cytokinins: kinetin (K), metatopolin (mT), $N^6$-benzyladenine (BA), and thidiazuron (TDZ), in equimolar concentrations, was investigated. Shoot formation from apical and basal pseudobulb sections was obtained in all treatments. A few medial sections cultured in media supplemented with BA formed protocorm-like bodies. Shoot formation was greater from the basal section than the apical, and mainly occurred in explants cultured in media containing TDZ. The highest average numbers of shoots per explant were achieved from basal sections cultured in media supplemented with TDZ at 4.4, 8.87 and 2.2 ${\mu}M$, forming on average 9.9, 8.6 and 7.3 shoots per explant, respectively. Since the medial pseudobulb section was the worst explant for propagation of L. aromatica, we recommend that pseudobulbs be divided into two sections; the basal half should be cultured in MS medium supplemented with TDZ at 4.4 ${\mu}M$ and the apical half with TDZ at 2.2 ${\mu}M$. Subculturing individual shoots in MS medium without plant growth regulators allows further development and rooting. A survival rate of more than 90% under greenhouse conditions was achieved. This research represents a direct contribution to the conservation and sustainable use of this valuable natural resource.

Keywords

References

  1. Arditti J (1992) Fundamentals of orchid biology. Wiley, USA
  2. Arditti J, Ernst R (1993) Micropropagation of orchids. Wiley, USA
  3. Campacci MA, Baptista DH, Cribb P, George A, Kreuz K, Wood J (2006) World checklist of Orchidaceae. The Board of Trustees of the Royal Botanic Gardens, Kew. http://www.kew.org/wcsp/ Accessed 31 March 2008; 10:41 GMT
  4. Chen JT, Chang C, Chang WC (1999) Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration. Plant Cell Rep 19:143-149 https://doi.org/10.1007/s002990050724
  5. Chen TY, Chen JT, Chang WC (2004) Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell Tissue Org Cult 76:11-15 https://doi.org/10.1023/A:1025858211320
  6. Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122: 507-520 https://doi.org/10.1016/j.scienta.2009.07.016
  7. Chung HH, Chen JT, Chang WC (2005) Cytokinins induce direct somatic embryogenesis of Dendrobium Chiengmai Pink and subsequent plant regeneration. In Vitro Cell Dev Biol Plant 41: 765-769 https://doi.org/10.1079/IVP2005702
  8. Convention on International Trade in Endangered Species ofWild Fauna and Flora (2009) Appendices I, II and III. http://www.cites.org/eng/app/e-appendices.pdf
  9. Fay MF (1994) In what situations is in vitro culture appropriate to plant conservation? Biodivers Conserv 3:176-183 https://doi.org/10.1007/BF02291887
  10. Flores-Palacios A, Valencia-Diaz S (2007) Local illegal trade reveals unknown diversity and involves a high species richness of wild vascular epiphytes. Biol Conserv 136:372-387 https://doi.org/10.1016/j.biocon.2006.12.017
  11. George EF (1993) Plant propagation by tissue culture. Exegetics, England
  12. George PS, Ravishankar GA (1997) In vitro multiplication of Vanilla planifolia using axillary bud explants. Plant Cell Rep 16:490-494
  13. George EF, Hall MA, De Klerk G (2008) The Background, 3rd edn. Plant propagation by tissue culture, vol 1. Springer, Dordrecht
  14. Govil S, Gupta S (1997) Commercialization of plant tissue culture in India. Plant Cell Tissue Org Cult 51:65-73 https://doi.org/10.1023/A:1005873221559
  15. Hagsater E, Salazar GA (1990) Icones Orchidacearum Fascicle 1. Orchids of Mexico Part 1. Asociacion Mexicana de Orquideologıa AC, Mexico
  16. Ishii Y, Takamura T, Goi M, Tanaka M (1998) Callus induction and somatic embryogenesis of Phalaenopsis. Plant Cell Rep 17:446-450 https://doi.org/10.1007/s002990050423
  17. Ket NV, Hahn EJ, Park SY, Chakrabarty D, Paek KY (2004) Micropropagation of an endangered orchid Anoectochilus formosanus. Biol Plant 48:339-344 https://doi.org/10.1023/B:BIOP.0000041084.77832.11
  18. KuoHL,Chen JT,ChangWC(2005) Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis 'little steve'. In Vitro Cell Dev Biol Plant 41:453-456 https://doi.org/10.1079/IVP2005644
  19. LuMC(2004) High frequency plant regeneration from callus culture of Pleione formosana Hayata. Plant Cell Tissue Org Cult 78:93-96
  20. Mata-Rosas M, Salazar-Rojas V (2009) Propagation and establishment of three endangered Mexican orchids from protocorms. HortScience 44:1395-1399
  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-494 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  22. Nayak NR, Patnaik S, Rath SP (1997) Direct shoot regeneration from foliar explants of an epiphytic orchid, Acampe praemorsa (Roxb.) Blatter and McCann. Plant Cell Rep 16:583-586
  23. Ogura-Tsujita Y, Okubo H (2006) Effects of low nitrogen medium on endogenous change in ethylene, auxins, and cytokinins in in vitro shoot formation from rhizome of Cymbidium kanran. In Vitro Cell Dev Biol Plant 42:614-616 https://doi.org/10.1079/IVP2006823
  24. Peres LEP, Amar S, Kerbauy GB, Salatino A, Zaffari GR, Mercier H (1999) Effects of auxin, cytokinin and ethylene treatments on the endogenous ethylene and auxin-to-cytokinins ratio related to direct root tip conversion of Catasetum fimbriatum Lindl. (Orchidaceae) into buds. J Plant Physiol 155:551-555 https://doi.org/10.1016/S0176-1617(99)80053-4
  25. Santos-Hernandez l, Martinez-Garcia M, Campo J, Aguirre-Leon E (2005) In vitro propagation of Laelia albida (Orchidaceae) for conservation and ornamental purposes in Mexico. Hortscience 40:439-442
  26. Shiau YJ, Nalawade SM, Hsia CN, Mulabacal V, Tsay HS (2005) In vitro propagation of the Chinese medicinal plant, Dendrobium candidum Wall. Ex Lindl., from axenic nodal segments. In Vitro Cell Dev Biol Plant 41:666-670 https://doi.org/10.1079/IVP2005685
  27. Shyamal K, Pinaki S (2004) Regeneration of an indigenous orchid, Vanda teres (Roxb.) Lindl. through in vitro culture. Plant Tissue Cult 14:55-62
  28. Tinoco MS, Mata M (2007) Adquisicion de competencia para la micropropagacion de Stanhopea tigrina, Laelia anceps, Epidendrum veroscriptum y Cattleya x Esbetts (Orchidaceae). Lankesteriana 7:404-418
  29. Tokuhara K, Mii M (2001) Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae). In Vitro Cell Dev Biol Plant 37:457-461 https://doi.org/10.1007/s11627-001-0080-4
  30. Zhao P, Wang W, Feng FS, Wu F, Yang ZQ, Wang WJ (2007) Highfrequency shoot regeneration through transverse thin cell layer culture in Dendrobium Candidum Wall Ex Lindl. Plant Cell Tissue Organ Cult 90:131-139 https://doi.org/10.1007/s11240-006-9181-4

Cited by

  1. Topolins: A panacea to plant tissue culture challenges? vol.108, pp.1, 2010, https://doi.org/10.1007/s11240-011-0007-7
  2. The Application of Biotechnology to Orchids vol.32, pp.2, 2013, https://doi.org/10.1080/07352689.2012.715984
  3. Multiple regeneration pathways in Spathoglottis plicata Blume - A study in vitro vol.85, pp.None, 2010, https://doi.org/10.1016/j.sajb.2012.12.005