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High frequency plant regeneration system for Nymphoides coreana via somatic embryogenesis from zygotic embryo-derived embryogenic cell suspension cultures

  • Oh, Myung-Jin (Biological Resource Center, Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Na, Hye-Ryun (Plant Systematics Laboratory, Department of Biological Science,Ajou University) ;
  • Choi, Hong-Keun (Plant Systematics Laboratory, Department of Biological Science,Ajou University) ;
  • Liu, Jang Ryol (Plant Systems Engineering Research Center, Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Suk-Weon (Biological Resource Center, Research Institute of Bioscience and Biotechnology (KRIBB))
  • Received : 2009.06.26
  • Accepted : 2010.01.12
  • Published : 2010.06.30

Abstract

Culture conditions were established for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Nymphoides coreana. Zygotic embryos formed pale-yellow globular structures and calluses at a frequency of 85.6% when cultured on half-strength Murashige and Skoog (MS) medium supplemented with 0.3 $mg\;l^{-1}$ of 2,4-D. However, the frequency of pale-yellow globular structures and white callus formation decreased slightly with an increasing concentration of 2,4-D up to 10 $mg\;l^{-1}$ with the frequency rate falling to 16.7%. Cell suspension cultures were established from zygotic embryo-derived calluses using half-strength MS medium supplemented with 0.3 $mg\;l^{-1}$ of 2,4-D. Upon plating onto half-strength MS basal medium, over 92.3% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted into potting soil and achieved full growth to an adult plant in a growth chamber. The high frequency plant regeneration system for Nymphoides coreana established in this study will be useful for genetic manipulation and cryopreservation of this species.

Keywords

References

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