DOI QR코드

DOI QR Code

Hypoglycemic effect of Rehmannie Radix Preparata (Sookjihwang) extract in streptozotocin-induced diabetic rats

  • Kang, Shin-Jyung (Department of Medicines Resources, Graduate School of Joongbu University) ;
  • Bao, Cun Liu (Department of Medicines Resources, Graduate School of Joongbu University) ;
  • Park, Soo-Jin (Department of Oriental Medical Food and Nutrition, Semyung University) ;
  • Kim, Ae-Jung (Department of Food & Nutrition, Hyejeon College)
  • 투고 : 2010.07.06
  • 심사 : 2010.09.09
  • 발행 : 2010.10.31

초록

Rhemannie Radix Preparata (RRP) has been previously employed in traditional oriental medicine as a treatment for diabetic thirst and improving blood flow. The aim of this study was to evaluate its hypoglycemic control by assaying the activities of key enzymes of carbohydrate metabolism in streptozotocin-(STZ)-induced diabetic rats. Further, RRP extracts were prepared in water (RRPW), in 50% ethanol (RRP50), and in 100% ethanol (RRP100), respectively, and compared for their actions in diabetic rats. The oral treatment of RRP (5 mg/kg b.w./d) to diabetic rats for 21 days resulted in a significant decline in blood glucose by 67% compared to diabetic control rats (P < 0.05). The altered activities of glucokinase, glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and acetyl CoA carboxylase (ACC) in the livers of diabetic rats were reversed significantly to near-normal levels by the administration of RRP (P < 0.05). Among the three RRP extracts, RRP100 was the most effective in terms of hypoglycemic action. However, the administration of RRP to diabetic rats did not improve insulin production. The modulatory effects of RRP100 on the attenuation of carbohydrate enzyme activities appear to hold promise for widespread use for the treatment of diabetes in the future.

키워드

참고문헌

  1. Yen KY. The illustrated Chinese Materia Medica. Crude and Prepared. Taipei: SMC Publishing Inc.; 1997. p.59.
  2. Yook CS, Moon YH, Lee KS. Korea phytology. Seoul: Yeolu moonwhasa Publishing Inc.; 1994. p.316.
  3. Lee CK, Seo JM. Changes of the constituents in the Rehamanniae Radix Preparata during processing. Journal of the Korean Society of Food Science and Nutrition 2004;22:1748-52.
  4. Lau TW, Lam FFY, Lau KM, Chan YW, Lee KM, Sahota DS, Ho YY, Fung KP, Leung PC, Lau CB. Pharmacological investigation on the wound healing effects of Radix Rehmanmiae in an animal model of diabetic foot ulcer. J Ethnopharmacol 2009;123:155-62. https://doi.org/10.1016/j.jep.2009.02.010
  5. Kim JO, Lee GD, Kwon JH, Kim KS. Anti-diabetic effects of New Herbal Formula in neonatally streptozotocin-induced diabetic rats. Biol Pharm Bull 2009;32:421-6. https://doi.org/10.1248/bpb.32.421
  6. Park SM, Hong SM, Sung SR, Lee JE, Kwon DY. Extracts of Rhemannia radix, Ginseng radix, and Scutellariae radix improve glucose-stimulated insulin secretion and beta cell proliferation through IRS2 induction. Genes Nutr 2008;2:347-51. https://doi.org/10.1007/s12263-007-0065-y
  7. Sasaki T, Matsy S, Sonae A. Effect of acetic acid concentration on the color reaction in the O-toluidine boric acid method for blood glucose estimation. Rinsho Kagak 1972;1:346-53.
  8. Joo CN, Koo JH, Lee HB. Study on the hypoglycemic action of the fat soluble fraction of Panax ginseng CA Meyer in streptozotocin induced rats. Korean Journal of Ginseng Science 1993;17:13-21.
  9. Yokozawa T, Kim HY, Yamabe N. Amelioration of diabetic nephropathy by dried Rehmanniae Radix (Di Huang) extract. Am J Chin Med 2004;32:829-39. https://doi.org/10.1142/S0192415X04002442
  10. Szkudelski T. The mechanism of alloxan and strptozotocin action in B cells of the rat pancrease. Physiol Res 2001;50:537-46.
  11. Laakso M, Malkki M, Deeb SS. Amino acid subetitutions in hexokinase II among patients with NIDDM. Diabetes 1995;44: 330-4. https://doi.org/10.2337/diabetes.44.3.330
  12. Singh J, Kakkar P. Antihyperglycemic and antioxidant effect of Berberis aristatta root extract and its role in regulating carbohydrate metabolism in diabetic rats. J Ethnopharmacol 2009;123:22-6. https://doi.org/10.1016/j.jep.2009.02.038
  13. Park MJ, Kang SJ, Kim AJ. Hypoglycemic effect of Angelica gigas Naki extracts in streptozotocin-induced diabetic rats. The Korean Journal of Food and Nutrition 2009;22:246-51.
  14. Xu Y, Osborne BW, Stanton RC. Diabetes causes inhibition of glucose-6-phosphate dehydrogenase via activation of PKA, which contributes to oxidative stress in rat kidney cortex. Am J Physiol Renal Physiol 2005;289:F1040-7. https://doi.org/10.1152/ajprenal.00076.2005
  15. Wagle A, Jivral S, Garlock GL, Stapleton SR. Insulin regulation of glucose 6- phosphate dehydregenase gene expression is rapamycinsensitive and requires phophatidylInositol 3-kinaes. J Biol Chem 1998;273:14968-74. https://doi.org/10.1074/jbc.273.24.14968
  16. Palsamy P, Subramanian S. Modulatory effects of resveratrol on attenuating the key-enzyme activities of carbohydrate metabolism in streptozotocin-nicotinamide-induced diabetic rats. Chem Biol Interact 2009;179:356-62. https://doi.org/10.1016/j.cbi.2008.11.008
  17. Niazi GA. Glucose-6-phosphate dehydrogenase deficiency and diabetes mellitus. Int J Hematol 1991;54:295-8.
  18. Livrea G, Carrozza G, Manasseri L, Muraca U. Concomitant repression by gluten of HMP shunt dehydrogenases, citrate cleavage enzyme and malic enzyme in rat liver and adipose tissue. J Nutr 1976;106:335-41.
  19. Tong L. Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery. Cell Mol Life Sci 2005; 62:1784-1803. https://doi.org/10.1007/s00018-005-5121-4
  20. Brownsey RW, Boone AN, Elliott JE, Kulpa JE, Lee WM. Regulation of acetyl-CoA carboxylase. Biochem Soc Trans 2006; 34:223-6. https://doi.org/10.1042/BST20060223

피인용 문헌

  1. A. Berger extracts in streptozotocin-induced diabetic rats vol.5, pp.4, 2011, https://doi.org/10.4162/nrp.2011.5.4.301
  2. Flaxseed Protects Against Diabetes-Induced Glucotoxicity by Modulating Pentose Phosphate Pathway and Glutathione-Dependent Enzyme Activities in Rats vol.13, pp.3, 2010, https://doi.org/10.3109/19390211.2015.1036188