Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
권호 표지
DOI QR코드

DOI QR Code

Protective effect of enzymatic hydrolysates from highbush blueberry (Vaccinium corymbosum L.) against hydrogen peroxide-induced oxidative damage in Chinese hamster lung fibroblast cell line

  • Senevirathne, Mahinda (School of Food Technology and Nutrition, Chonnam National University) ;
  • Kim, Soo-Hyun (Department of Food Bioengineering, Jeju National University) ;
  • Jeon, You-Jin (School of Marine Biomedical Science, Jeju National University)
  • Received : 2010.03.19
  • Accepted : 2010.05.27
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Blueberry was enzymatically hydrolyzed using selected commercial food grade carbohydrases (AMG, Celluclast, Termamyl, Ultraflo and Viscozyme) and proteases (Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex) to obtain water soluble compounds, and their protective effect was investigated against $H_2O_2$-induced damage in Chinese hamster lung fibroblast cell line (V79-4) via various published methods. Both AMG and Alcalase hydrolysates showed higher total phenolic content as well as higher cell viability and ROS scavenging activities, and hence, selected for further antioxidant assays. Both AMG and Alcalase hydrolysates also showed higher protective effects against lipid peroxidation, DNA damage and apoptotic body formation in a dose-dependent fashion. Thus, the results indicated that water soluble compounds obtained by enzymatic hydrolysis of blueberry possess good antioxidant activity against $H_2O_2$-induced cell damage in vitro.

Keywords

References

  1. LaFerla FM. Calcium dyshomeostasis and intracellular signaling in Alzheimer's disease. Nat Rev Neurosci 2002;11:862-72.
  2. Prasad KN, Cole WC, Hovland AR, Prasad KC, Nahreini P, Kumar B, Edwards-Prasad J, Andreatta CP. Multiple antioxidants in the prevention and treatment of neurodegenerative disease: analysis of biologic rationale. Curr Opin Neurol 1999;12:761-70. https://doi.org/10.1097/00019052-199912000-00017
  3. Bilici M, Efe H, Koroglu MA, Uydu HA, Bekaroglu M, Deger O. Antioxidative enzyme activities and lipid peroxidation in major depression: alterations by antidepressant treatments. J Affect Disord 2001;64:43-51. https://doi.org/10.1016/S0165-0327(00)00199-3
  4. Chandra J, Samali A, Orrenius S. Triggering and modulation of apoptosis by oxidative stress. Free Radic Biol Med 2001;29: 323-33.
  5. Triggiani V, Resta F, Guastamacchia E, Sabba C, Licchelli B, Ghiyasaldin S, Tafaro E. Role of antioxidants, essential fatty acids, carnitine, vitamins, phytochemicals and trace elements in the treatment of diabetes mellitus and its chronic complications. Endocr Metab Immune Disord Drug Targets 2006;6:77-93. https://doi.org/10.2174/187153006776056611
  6. De Flora, S, Ferguson LR. Overview of mechanisms of cancer chemopreventive agents. Mutat Res 2005;591:8-15.
  7. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  8. Prior RL, Cao G, Martin A, Sofic E, McEwen J, O'Brien C, Lischner N, Ehlenfeldt M, Kalt W, Krewer G, Mainland CM. Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species. J Agric Food Chem 1998;46:2686-93. https://doi.org/10.1021/jf980145d
  9. Neto CC. Cranberry and blueberry: evidence for protective effects against cancer and vascular diseases. Mol Nut Food Res 2007;51:652-64. https://doi.org/10.1002/mnfr.200600279
  10. Youdim KA, Shukitt-Hale B, Martin A, Wang H, Denisova N, Bickford PC, Joseph JA. Short-term dietary supplementation of blueberry polyphenolics: beneficial effects on aging brain performance and peripheral tissue function. Nutr Neurosci 2000; 3:383-97. https://doi.org/10.1080/1028415X.2000.11747338
  11. Gruenwald J. PDR for Herbal Medicines. Newjersy: Thomson PDR publisher; 2004. p.228-35.
  12. Chambers B, Camire M. Can cranberry supplementation benefit adults with type 2 diabetes? Diabetes Care 2003;26:2695-6. https://doi.org/10.2337/diacare.26.9.2695
  13. Heo SJ, Lee KW, Song CB, Jeon YJ. Antioxidant activity of enzymatic extracts from brown seaweeds. Algae 2003;18:71-81. https://doi.org/10.4490/ALGAE.2003.18.1.071
  14. Chandler SF, Dodds JH. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plant Cell Rep 1993;2:105-10.
  15. Hansen MB, Nielsen SE, Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 1989;119:203-10. https://doi.org/10.1016/0022-1759(89)90397-9
  16. Engelmann J, Volk J, Leyhausen G, Geurtsen W. ROS formation and glutathione levels in human oral fibroblasts exposed to TEGDMA and camphorquinone. J Biomed Mater Res Part B Appl Biomater 2005;75:272-6.
  17. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8. https://doi.org/10.1016/0003-2697(79)90738-3
  18. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-91. https://doi.org/10.1016/0014-4827(88)90265-0
  19. Gschwind M, Huber G. Apoptotic cell death induced by ${\beta}$-amyloid 1-42 peptide is cell type dependent. J Neurochem 1995;65:292-300.
  20. Manach C, Mazur A, Scalbert A. Polyphenols and prevention of cardiovascular diseases. Curr Opin Lipidol 2005;16:77-84. https://doi.org/10.1097/00041433-200502000-00013
  21. Schmidt BM, Howell AB, Mceniry B, Knight CT, Seigler D, Erdman JW Jr, Lila MA. Effective separation of potent antiproliferation and antiadhesion components from wild blueberry (Vaccinium angustifolium Ait.) fruits. J Agric Food Chem 2004;52:6433-42. https://doi.org/10.1021/jf049238n
  22. Yi W, Akoh CC, Fischer J, Krewer G. Effects of phenolic compounds in blueberries and muscadine grapes on HepG2 cell viability and apoptosis. Food Res Int 2006;39:628-38. https://doi.org/10.1016/j.foodres.2006.01.001
  23. Sellappan S, Akoh CC, Krewer G. Phenolic compounds and antioxidant capacity of Georgia-Grown blueberries and blackberries. J Agric Food Chem 2002;50:2432-8. https://doi.org/10.1021/jf011097r
  24. Rhee SG.. Redox signaling: hydrogen peroxide as intracellular messenger. Exp Mol Med 1999;31:53-9. https://doi.org/10.1038/emm.1999.9
  25. Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Oxford: Oxford University Press; 1989. p.96-8.
  26. Wang H, Joseph JA. Mechanisms of hydrogen peroxide-induced calcium dysregulation in PC12 cells. Free Radic Biol Med 2000;28:1222-31. https://doi.org/10.1016/S0891-5849(00)00241-0
  27. Tan S, Sagara Y, Liu Y, Maher P, Schubert D. The regulation of reactive oxygen species production during programmed cell death. J Cell Biol 1998;141:1423-32. https://doi.org/10.1083/jcb.141.6.1423
  28. Castillo MR, Babson JR. $Ca^{2+}$-dependent mechanisms of cell injury in cultured cortical neurons. Neuroscience 1998;86: 1133-44. https://doi.org/10.1016/S0306-4522(98)00070-0
  29. McGinnis KM, Wang KKW, Gnegy ME. Alterations of extracellular calcium elicit selective modes of cell death and protease activation in SH-SY5Y human neuroblastoma cells. J Neurochem 1999;72:1853-63.
  30. Mansouri A, Makris DP, Kefalas P. Determination of hydrogen peroxide scavenging activity of cinnamic and benzoic acids employing a highly sensitive peroxyoxalate chemiluminescence-based assay: Structure-activity relationships. J Pharm Biomed Anal 2005;39:22-6. https://doi.org/10.1016/j.jpba.2005.03.044
  31. Yoshikawa T, Naito Y, Kondo M. Food and disease. In: Hiramatsu M, Yoshikawa T, Inoue M, editors. Free radicals and diseases. New York: Plenum Press; 1997. p.11-9.
  32. Halliwell B, Gutteridge MC. Oxygen toxicity, oxygen radical, transition metals and disease. Biochem J 1984; 219:1-14. https://doi.org/10.1042/bj2190001
  33. Chaudhary AK, Nokubo M, Reddy GR, Yeola SN, Morrow JD, Blair IA, Marnett LJ. Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver. Science 1994;265: 1580-2. https://doi.org/10.1126/science.8079172
  34. Kassie F, Parzefall W, Knasmuller S. Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat Res 2000;463:13-31. https://doi.org/10.1016/S1383-5742(00)00041-7
  35. Wilms LC, Hollman PCH, Boots AW, Kleinjans JCS. Protection by quercetin and quercetin-rich fruit juice against induction of oxidative DNA damage and formation of BPDE-DNA adducts in human lymphocytes. Mutat Res 2005;582:155-62. https://doi.org/10.1016/j.mrgentox.2005.01.006
  36. Cozzi R, Ricordy R, Aglitti T, Gatta V, Perticone P, De Salvia R. Ascorbic acid and beta-carotene as modulators of oxidative damage. Carcinogenesis 1997;18:223-8. https://doi.org/10.1093/carcin/18.1.223
  37. Jang JH, Surh YJ. Protective effects of resveratrol on hydrogen peroxide-induced apoptosis in rat pheochromocytoma (PC12) cells. Mutat Res 2001;496:181-90. https://doi.org/10.1016/S1383-5718(01)00233-9
  38. Kerr JF, Gobe GC, Winterford CM, Harmon BV. Anatomical methods in cell death. In: Schwartz LM, Osborne BA, editors. Methods in cell biology. New York: Academic Press; 1995. p.1-27.
  39. Vicki S, Peter AH, Philip JC. Detecting and quantifying apoptosis in tissue sections. In: Brady JM, editor. Apoptosis methods and protocols. New Jersey: Humana Press; 2004. p.67-84.

Cited by

  1. vol.5, pp.6, 2011, https://doi.org/10.4162/nrp.2011.5.6.495
  2. Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage vol.2014, pp.1741-4288, 2014, https://doi.org/10.1155/2014/379890
  3. In vitro antioxidant and anti–inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts vol.4, pp.10, 2014, https://doi.org/10.12980/APJTB.4.2014C1008
  4. Molecular Mechanism and Health Role of Functional Ingredients in Blueberry for Chronic Disease in Human Beings vol.19, pp.9, 2018, https://doi.org/10.3390/ijms19092785
  5. H2O2 에 의해 유도된 HepG2 세포의 산화적 스트레스에 대한 신품종 방사선 돌연변이 블랙베리 γ-B201의 세포 보호 효과 vol.46, pp.3, 2010, https://doi.org/10.9721/kjfst.2014.46.3.384
  6. Evaluación de los arándanos como radioprotectores potenciales vol.17, pp.1, 2015, https://doi.org/10.17533/udea.penh.v17na02
  7. DNA protection, antioxidant and xanthine oxidase inhibition activities of polyphenol-enriched fraction of Berberis integerrima Bunge fruits vol.21, pp.4, 2010, https://doi.org/10.22038/ijbms.2018.26563.6506
  8. Enzyme-Assisted Extraction to Obtain Phenolic-Enriched Wine Lees with Enhanced Bioactivity in Hypertensive Rats vol.10, pp.4, 2010, https://doi.org/10.3390/antiox10040517