Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
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Distribution of ddr (DNA damage response) Genes among Species of Deinococcus

  • Lim, Sangyong (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Jung, Sunwook (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Joe, Minho (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Kim, Dongho (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute)
  • Received : 2010.08.31
  • Accepted : 2010.09.14
  • Published : 2010.09.30

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Abstract

The bacterium Deinococcus radiodurans is one of the most resistant organisms to the effects of ionizing radiation and other DNA-damaging agents. In this study, distributions of 10 ddr (DNA damage response) genes were investigated in 8 species of Deinococcus by polymerase chain reaction (PCR). We have compared the sequences of ddr genes of D. radiodurans, D. geothermalis and D. deserti, and selected primers which are suitable for the detection of ddr in different species of Deinococcus. A sequence homology search and PCR assay showed that ddrO, which encodes a global regulator of the radiation-desiccation response, was most well conserved in the Deinococcus lineage.

Keywords

Acknowledgement

Supported by : Ministry of Education, Science and Technology (MEST)