Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
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Study on the Mechanism of Radiation-induced MCP-1 Expression in RAW264.7 Macrophage Cells

RAW264.7 대식세포에서 방사선에 의한 MCP-1 발현 기작 연구

  • Jin, Chang Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Yong Dae (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Dae Seong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jeong, Il Yun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 진창현 (한국원자력연구원 정읍방사선과학연구소) ;
  • 박용대 (한국원자력연구원 정읍방사선과학연구소) ;
  • 최대성 (한국원자력연구원 정읍방사선과학연구소) ;
  • 정일윤 (한국원자력연구원 정읍방사선과학연구소)
  • Received : 2010.08.20
  • Accepted : 2010.09.14
  • Published : 2010.09.30

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Abstract

The purpose of this study was to investigate the expression mechanism of MCP-1 in gamma-irradiated RAW 264.7 macrophage cells. MCP-1 plays an important role in attracting monocyte to injured site at the early inflammation stage. However the production mechanism of MCP-1 by gamma-irradiation in RAW 264.7 macrophage cells was almost undiscovered. We found that MCP-1 was produced in RAW 264.7 macrophage cells by irradiation with 5 Gy. And these inceases were attenuated by specific inhibitors treatment, such as $NF-{\kappa}B$, JNK, ERK, JAK2, and Pyk2. These results indicate that radiation-induced MCP-1 production is mediated by MyD88- and TRIF-dependent pathways in RAW 264.7 macrophage cells. Furthermore, gamma-irradiation induced heme oxygenase-1 (HO-1) expression in RAW 264.7 macrophage cells. However this induction level was reduced before MCP-1 and $IFN-{\beta}$ production.

Keywords

Acknowledgement

Supported by : 농림수산식품부